Restriction map for
pFN21AE5055
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AlwI AlwNI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BalI BamHI
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraI DrdI DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HinfI HpaI HphI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MluNI MroI MscI MseI MunI
NaeI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 418 GTCTAC GT^[AC][GT]AC
AfaI 168 GTAC GT^AC
AflIII 356 ACGCGT A^C[GA][CT]GT
AhdI 514 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5 AGCGCT AGC^GCT
AspEI 514 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 427 CTCGGG C^[CT]CG[GA]G
AviII 388 TGCGCA TGC^GCA
BcgI 200 CGATGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 322 CTGGAG CTGGAG(16/14)
BsgI 118 CTGCAC GTGCAG(16/14)
BsmAI 367 GAGAC GTCTC(1/5)
BsmBI 367 GAGACG CGTCTC(1/5)
BsmFI 112 GTCCC GGGAC(10/14)
BsoBI 427 CTCGGG C^[CT]CG[GA]G
BspMI 102 ACCTGC ACCTGC(4/8)
BsrBI 469 GAGCGG CCGCTC(-3/-3)
BsrI 165 CCAGT ACTGG(1/-1)
BssSI 559 CACGAG CACGAG(-5/-1)
BstAPI 120 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 550 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 514 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5 AGCGCT AGC^GCT
EcoT14I 23 CCTTGG C^C[AT][AT]GG
FspI 388 TGCGCA TGC^GCA
MluI 356 ACGCGT A^CGCGT
MslI 57 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 448 CAGCGG C[AC]G^C[GT]G
RsaI 168 GTAC GT^AC
SbfI 180 CCTGCAGG CCTGCA^GG
SfaNI 196 GATGC GCATC(5/9)
Sse8387I 180 CCTGCAGG CCTGCA^GG
StyI 23 CCTTGG C^C[AT][AT]GG
TspRI 32 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 173 GTGCAC G^TGCAC
556 GTGCAC
ApaI 188 GGGCCC GGGCC^C
262 GGGCCC
ApaLI 173 GTGCAC G^TGCAC
556 GTGCAC
BanI 394 GGCGCC G^G[CT][GA]CC
455 GGCGCC
BanII 188 GGGCCC G[GA]GC[CT]^C
262 GGGCCC
BbeI 394 GGCGCC GGCGC^C
455 GGCGCC
BstF5I 62 GGATG GGATG(2/0)
195 GGATG
DdeI 30 CTCAG C^T[ACGT]AG
85 CTCAG
FokI 62 GGATG GGATG(9/13)
195 GGATG
KasI 394 GGCGCC G^GCGCC
455 GGCGCC
NarI 394 GGCGCC GG^CGCC
455 GGCGCC
PstI 39 CTGCAG CTGCA^G
181 CTGCAG
SfcI 39 CTGCAG C^T[GA][CT]AG
181 CTGCAG
SfoI 394 GGCGCC GGC^GCC
455 GGCGCC
TaqI 69 TCGA T^CGA
132 TCGA
TthHB8I 69 TCGA T^CGA
132 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BbvI BcnI BglI BmyI
BsaHI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI MnlI MspI MvaI
MvnI MwoI NciI NlaIV Sau96I
ScrFI TseI