Restriction map for
pFN21AE1657
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII BamHI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MboI MfeI MflI MluI MroI
MseI MunI NdeI NdeII NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 418 GAATTT [GA]^AATT[CT]
AflIII 184 ACGTGT A^C[GA][CT]GT
ApaI 165 GGGCCC GGGCC^C
ApoI 418 GAATTT [GA]^AATT[CT]
AvrII 60 CCTAGG C^CTAGG
BalI 494 TGGCCA TGG^CCA
BanI 6 GGCGCC G^G[CT][GA]CC
BbeI 6 GGCGCC GGCGC^C
BbsI 253 GAAGAC GAAGAC(2/6)
BbvCI 75 GCTGAGG CCTCAGC(-5/-2)
BbvI 54 GCTGC GCAGC(8/12)
BfaI 61 CTAG C^TAG
BlnI 60 CCTAGG C^CTAGG
BlpI 469 GCTGAGC GC^T[ACGT]AGC
BpmI 282 CTGGAG CTGGAG(16/14)
Bpu1102I 469 GCTGAGC GC^T[ACGT]AGC
BpuAI 253 GAAGAC GAAGAC(2/6)
BsaAI 183 TACGTG [CT]AC^GT[GA]
BsgI 273 CTGCAC GTGCAG(16/14)
BspMI 271 ACCTGC ACCTGC(4/8)
BsrDI 225 GCAATG GCAATG(2/0)
BsrFI 81 GCCGGC [GA]^CCGG[CT]
BstNI 291 CCAGG CC^[AT]GG
BstXI 104 CCACGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 469 GCTGAGC GC^T[ACGT]AGC
Cfr10I 81 GCCGGC [GA]^CCGG[CT]
DraII 286 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 494 TGGCCA [CT]^GGCC[GA]
EcoO109I 286 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 291 CCAGG ^CC[AT]GG
FauI 336 GCGGG CCCGC(4/6)
HinfI 237 GAGTC G^A[ACGT]TC
KasI 6 GGCGCC G^GCGCC
MaeI 61 CTAG C^TAG
MaeIII 427 GTGAC ^GT[ACGT]AC
MboII 253 GAAGA GAAGA(8/7)
MluNI 494 TGGCCA TGG^CCA
MscI 494 TGGCCA TGG^CCA
MslI 105 CACGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 12 CAGCGG C[AC]G^C[GT]G
MvaI 291 CCAGG CC^[AT]GG
NaeI 81 GCCGGC GCC^GGC
NarI 6 GGCGCC GG^CGCC
NcoI 359 CCATGG C^CATGG
NgoMIV 81 GCCGGC G^CCGGC
NlaIII 360 CATG CATG^
PflMI 156 CCATAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 237 GAGTC GAGTC(4/5)
SfaNI 502 GCATC GCATC(5/9)
SfoI 6 GGCGCC GGC^GCC
SmlI 206 CTCAAG C^T[CT][GA]AG
TseI 54 GCTGC G^C[AT]GC
Tsp45I 427 GTGAC ^GT[GC]AC
Tsp509I 419 AATT ^AATT
Van91I 156 CCATAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 431 CCAAAACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 309 GTAC GT^AC
354 GTAC
AvaI 168 CCCGGG C^[CT]CG[GA]G
454 CCCGGG
AvaII 215 GGACC G^G[AT]CC
245 GGACC
BanII 100 GAGCCC G[GA]GC[CT]^C
165 GGGCCC
BmyI 100 GAGCCC G[AGT]GC[ACT]^C
165 GGGCCC
BsmI 144 GCATTC GAATGC(1/-1)
460 GCATTC
BsoBI 168 CCCGGG C^[CT]CG[GA]G
454 CCCGGG
Bsp1286I 100 GAGCCC G[AGT]GC[ACT]^C
165 GGGCCC
Cac8I 37 GCGAGC GC[ACGT]^[ACGT]GC
81 GCCGGC
DsaI 68 CCGTGG C^C[GA][CT]GG
359 CCATGG
HgaI 29 GCGTC GACGC(5/10)
137 GCGTC
HphI 486 GGTGA GGTGA(8/7)
269 TCACC
RsaI 309 GTAC GT^AC
354 GTAC
SmaI 168 CCCGGG CCC^GGG
454 CCCGGG
TspRI 440 CAGTG CA[GC]TG[ACGT][ACGT]^
510 CACTG
XmaCI 168 CCCGGG C^CCGGG
454 CCCGGG
XmaI 168 CCCGGG C^CCGGG
454 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AcyI AluI BcnI
BsaHI BsaJI BsiYI BslI BsmFI
BssKI BstUI CfoI Cfr13I DdeI
EcoT14I Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
MaeII MnlI MspI MvnI MwoI
NciI NlaIV Sau96I ScrFI StyI