Restriction map for
pFN21AE3688
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsiEI BsiWI
Bsp1407I BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MluNI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 752 TCCGGA T^CCGGA
AcyI 1323 GGCGCC G[GA]^CG[CT]C
AflIII 114 ACACGT A^C[GA][CT]GT
AocI 1272 CCTGAGG CC^T[ACGT]AGG
ApaI 361 GGGCCC GGGCC^C
BbeI 1323 GGCGCC GGCGC^C
BbrPI 115 CACGTG CAC^GTG
BbsI 271 GAAGAC GAAGAC(2/6)
BbvCI 189 CCTCAGC CCTCAGC(-5/-2)
BcgI 962 GCACCTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1019 GCCCTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 271 GAAGAC GAAGAC(2/6)
BsaAI 115 CACGTG [CT]AC^GT[GA]
BsaHI 1323 GGCGCC G[GA]^CG[CT]C
BseAI 752 TCCGGA T^CCGGA
BsmBI 1373 GAGACG CGTCTC(1/5)
BsmI 1278 GAATGC GAATGC(1/-1)
BspDI 802 ATCGAT AT^CGAT
BspEI 752 TCCGGA T^CCGGA
BstXI 446 CCATAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1272 CCTGAGG CC^T[ACGT]AGG
ClaI 802 ATCGAT AT^CGAT
DsaI 47 CCATGG C^C[GA][CT]GG
EarI 1177 GAAGAG CTCTTC(1/4)
Eco81I 1272 CCTGAGG CC^T[ACGT]AGG
EcoT14I 47 CCATGG C^C[AT][AT]GG
HaeII 1323 GGCGCC [GA]GCGC^[CT]
Hin1I 1323 GGCGCC G[GA]^CG[CT]C
KasI 1323 GGCGCC G^GCGCC
Ksp632I 1177 GAAGAG CTCTTC(1/4)
MroI 752 TCCGGA T^CCGGA
NarI 1323 GGCGCC GG^CGCC
NcoI 47 CCATGG C^CATGG
NlaIII 48 CATG CATG^
PaeR7I 874 CTCGAG C^TCGAG
PmaCI 115 CACGTG CAC^GTG
PmlI 115 CACGTG CAC^GTG
PvuII 697 CAGCTG CAG^CTG
SacI 70 GAGCTC GAGCT^C
SfoI 1323 GGCGCC GGC^GCC
SmaI 1195 CCCGGG CCC^GGG
SmlI 874 CTCGAG C^T[CT][GA]AG
StyI 47 CCATGG C^C[AT][AT]GG
TfiI 843 GAATC G^A[AT]TC
Tsp509I 1237 AATT ^AATT
XhoI 874 CTCGAG C^TCGAG
XmaCI 1195 CCCGGG C^CCGGG
XmaI 1195 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 1108 CGCG CG^CG
1224 CGCG
AlwNI 157 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
631 CAGGAGCTG
BanI 961 GGCACC G^G[CT][GA]CC
1323 GGCGCC
BcnI 1195 CCCGG CC^[GC]GG
1196 CCGGG
BsaWI 504 TCCGGT [AT]^CCGG[AT]
752 TCCGGA
BsgI 931 GTGCAG GTGCAG(16/14)
1352 GTGCAG
BspMI 572 ACCTGC ACCTGC(4/8)
156 GCAGGT
BstAPI 156 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
386 GCAACTCTTGC
BstUI 1108 CGCG CG^CG
1224 CGCG
BstYI 78 GGATCT [GA]^GATC[CT]
479 GGATCT
DrdI 151 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
816 GACTCGGATGTC
MflI 78 GGATCT [GA]^GATC[CT]
479 GGATCT
MslI 924 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1090 CACAACGATG
MspA1I 309 CAGCGG C[AC]G^C[GT]G
697 CAGCTG
MvnI 1108 CGCG CG^CG
1224 CGCG
NaeI 331 GCCGGC GCC^GGC
495 GCCGGC
NciI 1195 CCCGG CC^[GC]GG
1196 CCGGG
NgoMIV 331 GCCGGC G^CCGGC
495 GCCGGC
Tsp45I 140 GTCAC ^GT[GC]AC
442 GTGAC
XhoII 78 GGATCT [GA]^GATC[CT]
479 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I