Restriction map for
pFN21AB7056
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp718I AviII AvrII
BbsI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EarI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NruI NsiI NspV PacI PflMI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SexAI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Van91I XbaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 1087 GACGTC GACGT^C
AccI 1090 GTCTAC GT^[AC][GT]AC
AhdI 1012 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1012 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 232 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 29 GGATCC G^GATCC
BbrPI 361 CACGTG CAC^GTG
BbvCI 321 CCTCAGC CCTCAGC(-5/-2)
BcgI 1415 GCAAGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 447 TGATCA T^GATCA
BpmI 340 CTGGAG CTGGAG(16/14)
BsaAI 361 CACGTG [CT]AC^GT[GA]
BsaI 763 GGTCTC GGTCTC(1/5)
BsgI 1441 CTGCAC GTGCAG(16/14)
BsmAI 764 GTCTC GTCTC(1/5)
BspMI 1439 ACCTGC ACCTGC(4/8)
Eam1105I 1012 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 114 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 447 TGATCA T^GATCA
MseI 1613 TTAA T^TAA
MslI 1368 CATGCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 481 CTCGAG C^TCGAG
PflFI 232 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 361 CACGTG CAC^GTG
PmlI 361 CACGTG CAC^GTG
PshAI 1419 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 586 GATGC GCATC(5/9)
StuI 1136 AGGCCT AGG^CCT
Tru9I 1613 TTAA T^TAA
Tsp509I 444 AATT ^AATT
Tth111I 232 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 593 CCAAGGAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 481 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AflIII 218 ACGTGT A^C[GA][CT]GT
362 ACGTGT
Alw44I 1279 GTGCAC G^TGCAC
1375 GTGCAC
ApaI 666 GGGCCC GGGCC^C
922 GGGCCC
ApaLI 1279 GTGCAC G^TGCAC
1375 GTGCAC
Asp700I 180 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1059 GAAGTACTTC
AspHI 1279 GTGCAC G[AT]GC[AT]^C
1375 GTGCAC
BalI 730 TGGCCA TGG^CCA
1640 TGGCCA
BanII 666 GGGCCC G[GA]GC[CT]^C
922 GGGCCC
BsiHKAI 1279 GTGCAC G[AT]GC[AT]^C
1375 GTGCAC
BsrI 168 CCAGT ACTGG(1/-1)
1398 CCAGT
BstAPI 87 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1235 GCAACATCTGC
DraII 89 AGGGCCT [GA]G^G[ACGT]CC[CT]
666 GGGCCCC
DrdI 1033 GACAAGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1081 GACGGCGACGTC
EcoO109I 89 AGGGCCT [GA]G^G[ACGT]CC[CT]
666 GGGCCCC
MluNI 730 TGGCCA TGG^CCA
1640 TGGCCA
MscI 730 TGGCCA TGG^CCA
1640 TGGCCA
NotI 523 GCGGCCGC GC^GGCCGC
1490 GCGGCCGC
NspI 1283 ACATGC [GA]CATG^[CT]
1367 ACATGC
PstI 162 CTGCAG CTGCA^G
958 CTGCAG
PvuII 424 CAGCTG CAG^CTG
1407 CAGCTG
ScaI 170 AGTACT AGT^ACT
1061 AGTACT
SfcI 162 CTGCAG C^T[GA][CT]AG
958 CTGCAG
SgrAI 776 CGCCGGTG C[GA]^CCGG[CT]G
1680 CGCCGGTG
SmlI 481 CTCGAG C^T[CT][GA]AG
1453 CTCAAG
TaqI 230 TCGA T^CGA
482 TCGA
Tsp45I 314 GTGAC ^GT[GC]AC
1694 GTGAC
TspRI 1324 CAGTG CA[GC]TG[ACGT][ACGT]^
1399 CAGTG
TthHB8I 230 TCGA T^CGA
482 TCGA
XmnI 180 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1059 GAAGTACTTC
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BbeI BbvI BcnI BglI BmyI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EclXI Eco52I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PleI RsaI
SacII Sau3AI Sau96I ScrFI SfiI
SfoI SmaI StyI TseI XhoII
XmaCI XmaI