Restriction map for
pFN21AB6670
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EarI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I KspI MaeI MaeIII
MamI MfeI MroI MslI MunI
NcoI NdeI NheI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp45I
Tsp509I TspRI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1097 GGTACC G^GTACC
AcyI 884 GGCGCC G[GA]^CG[CT]C
AhdI 894 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 968 AGCGCT AGC^GCT
Asp718I 1097 GGTACC G^GTACC
AspEI 894 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 298 GAGCTC G[AT]GC[AT]^C
AspI 262 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 874 CCCGAG C^[CT]CG[GA]G
BanII 298 GAGCTC G[GA]GC[CT]^C
BbeI 884 GGCGCC GGCGC^C
BbsI 891 GAAGAC GAAGAC(2/6)
BglI 873 GCCCGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 891 GAAGAC GAAGAC(2/6)
BsaHI 884 GGCGCC G[GA]^CG[CT]C
BsiHKAI 298 GAGCTC G[AT]GC[AT]^C
BsmBI 670 GAGACG CGTCTC(1/5)
BsmFI 1213 GGGAC GGGAC(10/14)
BsoBI 874 CCCGAG C^[CT]CG[GA]G
BspLU11I 596 ACATGT A^CATGT
BsrDI 795 CATTGC GCAATG(2/0)
BstAPI 1146 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 894 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 968 AGCGCT AGC^GCT
Hin1I 884 GGCGCC G[GA]^CG[CT]C
KasI 884 GGCGCC G^GCGCC
KpnI 1097 GGTACC GGTAC^C
MluI 898 ACGCGT A^CGCGT
MseI 824 TTAA T^TAA
NarI 884 GGCGCC GG^CGCC
NspI 596 ACATGT [GA]CATG^[CT]
PflFI 262 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 801 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 325 GAGTC GAGTC(4/5)
PvuI 74 CGATCG CGAT^CG
SacI 298 GAGCTC GAGCT^C
ScaI 716 AGTACT AGT^ACT
SfoI 884 GGCGCC GGC^GCC
StuI 1091 AGGCCT AGG^CCT
TfiI 467 GAATC G^A[AT]TC
Tru9I 824 TTAA T^TAA
Tth111I 262 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 801 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 596 ACATGT A^C[GA][CT]GT
898 ACGCGT
Asp700I 513 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
525 GAACATCTTC
BalI 740 TGGCCA TGG^CCA
1268 TGGCCA
BcnI 171 CCGGG CC^[GC]GG
540 CCCGG
BsaI 658 GAGACC GGTCTC(1/5)
285 GGTCTC
BspMI 386 ACCTGC ACCTGC(4/8)
1130 ACCTGC
BsrI 66 CCAGT ACTGG(1/-1)
351 CCAGT
BssHII 1202 GCGCGC G^CGCGC
1314 GCGCGC
BssSI 1048 CACGAG CACGAG(-5/-1)
1309 CTCGTG
BstXI 731 CCAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
977 CCAAGGTGCTGG
EagI 864 CGGCCG C^GGCCG
998 CGGCCG
EclXI 864 CGGCCG C^GGCCG
998 CGGCCG
Eco52I 864 CGGCCG C^GGCCG
998 CGGCCG
EcoNI 1119 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1320 CCTGGAGCAGG
HgaI 672 GACGC GACGC(5/10)
900 GCGTC
HinfI 325 GAGTC G^A[ACGT]TC
467 GAATC
MluNI 740 TGGCCA TGG^CCA
1268 TGGCCA
MscI 740 TGGCCA TGG^CCA
1268 TGGCCA
NciI 171 CCGGG CC^[GC]GG
540 CCCGG
NlaIII 435 CATG CATG^
597 CATG
NotI 863 GCGGCCGC GC^GGCCGC
997 GCGGCCGC
PpuMI 1127 AGGACCT [GA]G^G[AT]CC[CT]
1304 AGGACCT
PstI 1024 CTGCAG CTGCA^G
1336 CTGCAG
SfcI 1024 CTGCAG C^T[GA][CT]AG
1336 CTGCAG
XmnI 513 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
525 GAACATCTTC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanI BbvI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI Bsp1286I BsrBI
BsrFI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NdeII
NgoMIV NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TseI TthHB8I XhoII