Restriction map for
pFN21AE5684
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# NO CUT ENZYMES ( 101 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BamHI BbrPI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MamI
MfeI MflI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XhoII
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1410 GACGTC GACGT^C
Acc65I 671 GGTACC G^GTACC
AlwNI 172 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 424 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1298 AGCGCT AGC^GCT
Asp718I 671 GGTACC G^GTACC
BbeI 1120 GGCGCC GGCGC^C
BbsI 637 GAAGAC GAAGAC(2/6)
BbvCI 1311 CCTCAGC CCTCAGC(-5/-2)
BcgI 1556 GCATCCGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 637 GAAGAC GAAGAC(2/6)
BsaAI 991 CACGTA [CT]AC^GT[GA]
BseRI 1525 CTCCTC GAGGAG(10/8)
BsiEI 963 CGGCCG CG[GA][CT]^CG
BsmI 358 GAATGC GAATGC(1/-1)
BsrDI 252 CATTGC GCAATG(2/0)
BstAPI 1511 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 424 CCTGAGG CC^T[ACGT]AGG
DrdI 799 GACCTGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 963 CGGCCG C^GGCCG
EclXI 963 CGGCCG C^GGCCG
Eco47III 1298 AGCGCT AGC^GCT
Eco52I 963 CGGCCG C^GGCCG
Eco81I 424 CCTGAGG CC^T[ACGT]AGG
EcoNI 115 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1120 GGCGCC G^GCGCC
KpnI 671 GGTACC GGTAC^C
NaeI 945 GCCGGC GCC^GGC
NarI 1120 GGCGCC GG^CGCC
NgoMIV 945 GCCGGC G^CCGGC
PaeR7I 1189 CTCGAG C^TCGAG
PstI 1518 CTGCAG CTGCA^G
SacI 498 GAGCTC GAGCT^C
SfiI 735 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1120 GGCGCC GGC^GCC
SmaI 737 CCCGGG CCC^GGG
TfiI 442 GATTC G^A[AT]TC
Tsp509I 15 AATT ^AATT
XbaI 393 TCTAGA T^CTAGA
XhoI 1189 CTCGAG C^TCGAG
XmaCI 737 CCCGGG C^CCGGG
XmaI 737 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AfaI 672 GTAC GT^AC
1719 GTAC
Alw44I 1105 GTGCAC G^TGCAC
1183 GTGCAC
AlwI 23 GATCC GGATC(4/5)
821 GGATC
ApaLI 1105 GTGCAC G^TGCAC
1183 GTGCAC
BalI 1035 TGGCCA TGG^CCA
1604 TGGCCA
BfaI 394 CTAG C^TAG
541 CTAG
BglI 736 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1094 GCCAGGTGGGC
BlpI 478 GCTGAGC GC^T[ACGT]AGC
1294 GCTGAGC
BpmI 38 CTCCAG CTGGAG(16/14)
526 CTGGAG
Bpu1102I 478 GCTGAGC GC^T[ACGT]AGC
1294 GCTGAGC
BsaI 583 GAGACC GGTCTC(1/5)
775 GAGACC
BsgI 1279 CTGCAC GTGCAG(16/14)
181 GTGCAG
BspMI 332 ACCTGC ACCTGC(4/8)
1274 ACCTGC
BsrFI 945 GCCGGC [GA]^CCGG[CT]
1466 ACCGGC
CelII 478 GCTGAGC GC^T[ACGT]AGC
1294 GCTGAGC
Cfr10I 945 GCCGGC [GA]^CCGG[CT]
1466 ACCGGC
EarI 385 GAAGAG CTCTTC(1/4)
553 GAAGAG
HaeII 1120 GGCGCC [GA]GCGC^[CT]
1298 AGCGCT
Ksp632I 385 GAAGAG CTCTTC(1/4)
553 GAAGAG
MaeI 394 CTAG C^TAG
541 CTAG
MaeII 992 ACGT A^CGT
1411 ACGT
MluNI 1035 TGGCCA TGG^CCA
1604 TGGCCA
MscI 1035 TGGCCA TGG^CCA
1604 TGGCCA
MslI 69 CATTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
720 CACGCGCATG
PflMI 264 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
559 CCACCCGCTGG
RsaI 672 GTAC GT^AC
1719 GTAC
SapI 385 GAAGAGC GCTCTTC(1/4)
553 GAAGAGC
SfcI 1056 CTACAG C^T[GA][CT]AG
1518 CTGCAG
SmlI 501 CTCAAG C^T[CT][GA]AG
1189 CTCGAG
StuI 1040 AGGCCT AGG^CCT
1643 AGGCCT
TaqI 1190 TCGA T^CGA
1628 TCGA
TthHB8I 1190 TCGA T^CGA
1628 TCGA
Van91I 264 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
559 CCACCCGCTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AspI AvaI AvaII BanI
BanII BbvI BcnI BmyI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PvuII Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
Tth111I XcmI