Restriction map for
pFN21AE1763
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AvrII BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 404 ACACGT A^C[GA][CT]GT
AluI 527 AGCT AG^CT
AlwNI 736 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 128 AGCGCT AGC^GCT
ApaI 305 GGGCCC GGGCC^C
AspI 277 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 101 TGCGCA TGC^GCA
BalI 212 TGGCCA TGG^CCA
BamHI 392 GGATCC G^GATCC
BanII 305 GGGCCC G[GA]GC[CT]^C
BbsI 283 GTCTTC GAAGAC(2/6)
BcgI 209 GCATGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 305 GGGCCC G[AGT]GC[ACT]^C
BpuAI 283 GTCTTC GAAGAC(2/6)
BsaAI 405 CACGTA [CT]AC^GT[GA]
BsgI 232 GTGCAG GTGCAG(16/14)
Bsp1286I 305 GGGCCC G[AGT]GC[ACT]^C
BspMI 98 ACCTGC ACCTGC(4/8)
BsrDI 272 GCAATG GCAATG(2/0)
BsrFI 117 GCCGGT [GA]^CCGG[CT]
BstAPI 735 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 773 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 392 GGATCC [GA]^GATC[CT]
Cfr10I 117 GCCGGT [GA]^CCGG[CT]
Eco47III 128 AGCGCT AGC^GCT
EcoNI 345 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 101 TGCGCA TGC^GCA
KspI 71 CCGCGG CCGC^GG
MaeII 406 ACGT A^CGT
MflI 392 GGATCC [GA]^GATC[CT]
MluNI 212 TGGCCA TGG^CCA
MscI 212 TGGCCA TGG^CCA
NcoI 591 CCATGG C^CATGG
PflFI 277 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 45 CTGCAG CTGCA^G
SacII 71 CCGCGG CCGC^GG
ScaI 542 AGTACT AGT^ACT
SfaNI 104 GCATC GCATC(5/9)
SfcI 45 CTGCAG C^T[GA][CT]AG
StuI 342 AGGCCT AGG^CCT
TaqI 266 TCGA T^CGA
Tth111I 277 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 266 TCGA T^CGA
XhoII 392 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaII 189 GGTCC G^G[AT]CC
664 GGTCC
BbeI 66 GGCGCC GGCGC^C
240 GGCGCC
BseRI 171 CTCCTC GAGGAG(10/8)
421 GAGGAG
BsmFI 299 GGGAC GGGAC(10/14)
665 GTCCC
BsrI 302 ACTGG ACTGG(1/-1)
441 CCAGT
DdeI 546 CTCAG C^T[ACGT]AG
758 CTCAG
DpnII 261 GATC ^GATC
393 GATC
EaeI 158 TGGCCG [CT]^GGCC[GA]
212 TGGCCA
EcoT14I 591 CCATGG C^C[AT][AT]GG
612 CCTTGG
FauI 52 CCCGC CCCGC(4/6)
70 CCCGC
KasI 66 GGCGCC G^GCGCC
240 GGCGCC
MaeIII 380 GTCAC ^GT[ACGT]AC
608 GTGAC
MboI 261 GATC ^GATC
393 GATC
MboII 26 TCTTC GAAGA(8/7)
284 TCTTC
MslI 382 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
555 CACCATCATG
NarI 66 GGCGCC GG^CGCC
240 GGCGCC
NdeII 261 GATC ^GATC
393 GATC
PpuMI 188 GGGTCCT [GA]G^G[AT]CC[CT]
663 GGGTCCC
Sau3AI 261 GATC ^GATC
393 GATC
SfoI 66 GGCGCC GGC^GCC
240 GGCGCC
StyI 591 CCATGG C^C[AT][AT]GG
612 CCTTGG
Tsp45I 380 GTCAC ^GT[GC]AC
608 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AfaI AlwI
BanI BbvI BcnI BpmI BsaHI
BsaI BsaJI BsiYI BslI BsmAI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DraII DsaI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
TseI TspRI