Restriction map for
pFN21AE9550
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AclI AcsI
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbsI
BcgI BciVI BclI BfaI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BseRI BsiWI BsmBI BsmI BspDI
BspHI BspLU11I BsrDI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI DpnII DraI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HinfI HpaI KpnI MaeI
MamI MboI MfeI MflI MluNI
MscI MunI NcoI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI SacI SalI Sau3AI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI SspI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 19 TCCGGA T^CCGGA
AflII 910 CTTAAG C^TTAAG
AspHI 529 GTGCTC G[AT]GC[AT]^C
BbrPI 384 CACGTG CAC^GTG
BbvCI 489 CCTCAGC CCTCAGC(-5/-2)
BfrI 910 CTTAAG C^TTAAG
BpmI 598 CTGGAG CTGGAG(16/14)
BsaWI 19 TCCGGA [AT]^CCGG[AT]
BseAI 19 TCCGGA T^CCGGA
BsiEI 797 CGGCCG CG[GA][CT]^CG
BsiHKAI 529 GTGCTC G[AT]GC[AT]^C
Bsp1407I 617 TGTACA T^GTACA
BspEI 19 TCCGGA T^CCGGA
BsrBI 136 CCGCTC CCGCTC(-3/-3)
BsrGI 617 TGTACA T^GTACA
BsrI 97 CCAGT ACTGG(1/-1)
BssSI 130 CTCGTG CACGAG(-5/-1)
BstXI 404 CCATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 220 CGGACCG CG^G[AT]CCG
DdeI 490 CTCAG C^T[ACGT]AG
DraIII 4 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 91 GACGGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 797 CGGCCG C^GGCCG
EclXI 797 CGGCCG C^GGCCG
Eco52I 797 CGGCCG C^GGCCG
EcoNI 1170 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 776 CCAAGG C^C[AT][AT]GG
HincII 352 GTCAAC GT[CT]^[GA]AC
HindII 352 GTCAAC GT[CT]^[GA]AC
HphI 550 TCACC GGTGA(8/7)
MluI 29 ACGCGT A^CGCGT
MroI 19 TCCGGA T^CCGGA
MseI 911 TTAA T^TAA
NaeI 1046 GCCGGC GCC^GGC
NgoMIV 1046 GCCGGC G^CCGGC
NspI 929 GCATGT [GA]CATG^[CT]
PmaCI 384 CACGTG CAC^GTG
PmlI 384 CACGTG CAC^GTG
PpuMI 1099 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 838 CAGCTG CAG^CTG
RsrII 220 CGGACCG CG^G[AT]CCG
SbfI 732 CCTGCAGG CCTGCA^GG
SgrAI 524 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 910 CTTAAG C^T[CT][GA]AG
Sse8387I 732 CCTGCAGG CCTGCA^GG
SspBI 617 TGTACA T^GTACA
StyI 776 CCAAGG C^C[AT][AT]GG
Tru9I 911 TTAA T^TAA
XcmI 587 CCAGCCGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 29 ACGCGT A^C[GA][CT]GT
385 ACGTGT
ApaI 828 GGGCCC GGGCC^C
864 GGGCCC
AvaI 66 CCCGGG C^[CT]CG[GA]G
553 CCCGGG
BbeI 76 GGCGCC GGCGC^C
763 GGCGCC
BsaAI 384 CACGTG [CT]AC^GT[GA]
424 TACGTG
BsaI 369 GGTCTC GGTCTC(1/5)
780 GGTCTC
BsmAI 370 GTCTC GTCTC(1/5)
781 GTCTC
BsoBI 66 CCCGGG C^[CT]CG[GA]G
553 CCCGGG
BstF5I 360 CATCC GGATG(2/0)
834 CATCC
EarI 139 CTCTTC CTCTTC(1/4)
811 CTCTTC
FokI 360 CATCC GGATG(9/13)
834 CATCC
KasI 76 GGCGCC G^GCGCC
763 GGCGCC
Ksp632I 139 CTCTTC CTCTTC(1/4)
811 CTCTTC
KspI 109 CCGCGG CCGC^GG
869 CCGCGG
MslI 258 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
405 CATGAGTGTG
NarI 76 GGCGCC GG^CGCC
763 GGCGCC
SacII 109 CCGCGG CCGC^GG
869 CCGCGG
SapI 138 GCTCTTC GCTCTTC(1/4)
810 GCTCTTC
SfoI 76 GGCGCC GGC^GCC
763 GGCGCC
SmaI 66 CCCGGG CCC^GGG
553 CCCGGG
StuI 578 AGGCCT AGG^CCT
995 AGGCCT
XmaCI 66 CCCGGG C^CCGGG
553 CCCGGG
XmaI 66 CCCGGG C^CCGGG
553 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BanI BanII BbvI
BcnI BglI BmyI BsaHI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BsrFI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MaeII MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PstI RsaI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI