Restriction map for
pFN21AE1750
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BalI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstF5I BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HindIII HpaI KpnI Ksp632I KspI
MamI MboII MfeI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 282 GTAC GT^AC
Aor51HI 572 AGCGCT AGC^GCT
AspI 745 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 36 CCTAGG C^CTAGG
BamHI 74 GGATCC G^GATCC
BbrPI 502 CACGTG CAC^GTG
BfaI 37 CTAG C^TAG
BglI 49 GCCTGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 36 CCTAGG C^CTAGG
BlpI 183 GCTGAGC GC^T[ACGT]AGC
BpmI 202 CTGGAG CTGGAG(16/14)
Bpu1102I 183 GCTGAGC GC^T[ACGT]AGC
BsaAI 502 CACGTG [CT]AC^GT[GA]
BsmFI 602 GGGAC GGGAC(10/14)
BsrBI 41 GAGCGG CCGCTC(-3/-3)
BstEII 663 GGTCACC G^GT[ACGT]ACC
BstPI 663 GGTCACC G^GT[ACGT]ACC
CelII 183 GCTGAGC GC^T[ACGT]AGC
DdeI 184 CTGAG C^T[ACGT]AG
DsaI 66 CCGTGG C^C[GA][CT]GG
Eco47III 572 AGCGCT AGC^GCT
EcoO65I 663 GGTCACC G^GT[ACGT]ACC
EcoT14I 36 CCTAGG C^C[AT][AT]GG
HincII 466 GTCAAC GT[CT]^[GA]AC
HindII 466 GTCAAC GT[CT]^[GA]AC
HinfI 619 GACTC G^A[ACGT]TC
MaeI 37 CTAG C^TAG
MslI 714 CACCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 451 GCCGGC GCC^GGC
NgoMIV 451 GCCGGC G^CCGGC
NlaIII 117 CATG CATG^
NotI 43 GCGGCCGC GC^GGCCGC
PaeR7I 652 CTCGAG C^TCGAG
PflFI 745 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 619 GACTC GAGTC(4/5)
PmaCI 502 CACGTG CAC^GTG
PmlI 502 CACGTG CAC^GTG
PstI 496 CTGCAG CTGCA^G
RsaI 282 GTAC GT^AC
SmaI 703 CCCGGG CCC^GGG
SmlI 652 CTCGAG C^T[CT][GA]AG
StyI 36 CCTAGG C^C[AT][AT]GG
Tth111I 745 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 652 CTCGAG C^TCGAG
XmaCI 703 CCCGGG C^CCGGG
XmaI 703 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 378 GGGCCC GGGCC^C
627 GGGCCC
BbeI 57 GGCGCC GGCGC^C
559 GGCGCC
BsgI 127 GTGCAG GTGCAG(16/14)
226 GTGCAG
BsiEI 44 CGGCCG CG[GA][CT]^CG
212 CGGCCG
BspMI 532 ACCTGC ACCTGC(4/8)
228 GCAGGT
BsrFI 451 GCCGGC [GA]^CCGG[CT]
539 ACCGGC
BsrI 375 ACTGG ACTGG(1/-1)
514 CCAGT
BssHII 140 GCGCGC G^CGCGC
242 GCGCGC
BstAPI 174 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
498 GCAGCACGTGC
BstYI 74 GGATCC [GA]^GATC[CT]
707 GGATCT
Cfr10I 451 GCCGGC [GA]^CCGG[CT]
539 ACCGGC
DraII 378 GGGCCCT [GA]G^G[ACGT]CC[CT]
626 GGGGCCC
EagI 44 CGGCCG C^GGCCG
212 CGGCCG
EclXI 44 CGGCCG C^GGCCG
212 CGGCCG
Eco52I 44 CGGCCG C^GGCCG
212 CGGCCG
EcoO109I 378 GGGCCCT [GA]G^G[ACGT]CC[CT]
626 GGGGCCC
KasI 57 GGCGCC G^GCGCC
559 GGCGCC
MaeIII 617 GTGAC ^GT[ACGT]AC
664 GTCAC
MflI 74 GGATCC [GA]^GATC[CT]
707 GGATCT
NarI 57 GGCGCC GG^CGCC
559 GGCGCC
SfcI 352 CTACAG C^T[GA][CT]AG
496 CTGCAG
SfoI 57 GGCGCC GGC^GCC
559 GGCGCC
Tsp45I 617 GTGAC ^GT[GC]AC
664 GTCAC
XhoII 74 GGATCC [GA]^GATC[CT]
707 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV PvuII Sau3AI Sau96I
ScrFI TaqI TseI TspRI TthHB8I