################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII BalI BbsI BbvCI BcgI BciVI BclI BfrI BglII BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstF5I BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HindIII HpaI KpnI Ksp632I KspI MamI MboII MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AfaI 282 GTAC GT^AC Aor51HI 572 AGCGCT AGC^GCT AspI 745 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 36 CCTAGG C^CTAGG BamHI 74 GGATCC G^GATCC BbrPI 502 CACGTG CAC^GTG BfaI 37 CTAG C^TAG BglI 49 GCCTGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 36 CCTAGG C^CTAGG BlpI 183 GCTGAGC GC^T[ACGT]AGC BpmI 202 CTGGAG CTGGAG(16/14) Bpu1102I 183 GCTGAGC GC^T[ACGT]AGC BsaAI 502 CACGTG [CT]AC^GT[GA] BsmFI 602 GGGAC GGGAC(10/14) BsrBI 41 GAGCGG CCGCTC(-3/-3) BstEII 663 GGTCACC G^GT[ACGT]ACC BstPI 663 GGTCACC G^GT[ACGT]ACC CelII 183 GCTGAGC GC^T[ACGT]AGC DdeI 184 CTGAG C^T[ACGT]AG DsaI 66 CCGTGG C^C[GA][CT]GG Eco47III 572 AGCGCT AGC^GCT EcoO65I 663 GGTCACC G^GT[ACGT]ACC EcoT14I 36 CCTAGG C^C[AT][AT]GG HincII 466 GTCAAC GT[CT]^[GA]AC HindII 466 GTCAAC GT[CT]^[GA]AC HinfI 619 GACTC G^A[ACGT]TC MaeI 37 CTAG C^TAG MslI 714 CACCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 451 GCCGGC GCC^GGC NgoMIV 451 GCCGGC G^CCGGC NlaIII 117 CATG CATG^ NotI 43 GCGGCCGC GC^GGCCGC PaeR7I 652 CTCGAG C^TCGAG PflFI 745 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 619 GACTC GAGTC(4/5) PmaCI 502 CACGTG CAC^GTG PmlI 502 CACGTG CAC^GTG PstI 496 CTGCAG CTGCA^G RsaI 282 GTAC GT^AC SmaI 703 CCCGGG CCC^GGG SmlI 652 CTCGAG C^T[CT][GA]AG StyI 36 CCTAGG C^C[AT][AT]GG Tth111I 745 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 652 CTCGAG C^TCGAG XmaCI 703 CCCGGG C^CCGGG XmaI 703 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### ApaI 378 GGGCCC GGGCC^C 627 GGGCCC BbeI 57 GGCGCC GGCGC^C 559 GGCGCC BsgI 127 GTGCAG GTGCAG(16/14) 226 GTGCAG BsiEI 44 CGGCCG CG[GA][CT]^CG 212 CGGCCG BspMI 532 ACCTGC ACCTGC(4/8) 228 GCAGGT BsrFI 451 GCCGGC [GA]^CCGG[CT] 539 ACCGGC BsrI 375 ACTGG ACTGG(1/-1) 514 CCAGT BssHII 140 GCGCGC G^CGCGC 242 GCGCGC BstAPI 174 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 498 GCAGCACGTGC BstYI 74 GGATCC [GA]^GATC[CT] 707 GGATCT Cfr10I 451 GCCGGC [GA]^CCGG[CT] 539 ACCGGC DraII 378 GGGCCCT [GA]G^G[ACGT]CC[CT] 626 GGGGCCC EagI 44 CGGCCG C^GGCCG 212 CGGCCG EclXI 44 CGGCCG C^GGCCG 212 CGGCCG Eco52I 44 CGGCCG C^GGCCG 212 CGGCCG EcoO109I 378 GGGCCCT [GA]G^G[ACGT]CC[CT] 626 GGGGCCC KasI 57 GGCGCC G^GCGCC 559 GGCGCC MaeIII 617 GTGAC ^GT[ACGT]AC 664 GTCAC MflI 74 GGATCC [GA]^GATC[CT] 707 GGATCT NarI 57 GGCGCC GG^CGCC 559 GGCGCC SfcI 352 CTACAG C^T[GA][CT]AG 496 CTGCAG SfoI 57 GGCGCC GGC^GCC 559 GGCGCC Tsp45I 617 GTGAC ^GT[GC]AC 664 GTCAC XhoII 74 GGATCC [GA]^GATC[CT] 707 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PvuII Sau3AI Sau96I ScrFI TaqI TseI TspRI TthHB8I