Restriction map for
pFN21AE1752
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BseRI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AfaI 114 GTAC GT^AC
AflIII 734 ACGTGT A^C[GA][CT]GT
Alw44I 166 GTGCAC G^TGCAC
AlwNI 681 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 166 GTGCAC G^TGCAC
AspHI 166 GTGCAC G[AT]GC[AT]^C
BalI 713 TGGCCA TGG^CCA
BbsI 15 GTCTTC GAAGAC(2/6)
BbvCI 222 GCTGAGG CCTCAGC(-5/-2)
BglI 34 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 15 GTCTTC GAAGAC(2/6)
BsaAI 733 TACGTG [CT]AC^GT[GA]
BsaI 262 GGTCTC GGTCTC(1/5)
BsiHKAI 166 GTGCAC G[AT]GC[AT]^C
BsmAI 263 GTCTC GTCTC(1/5)
BsrI 398 CCAGT ACTGG(1/-1)
BstAPI 566 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 753 GGTCACC G^GT[ACGT]ACC
BstPI 753 GGTCACC G^GT[ACGT]ACC
BstXI 716 CCACCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 223 CTGAG C^T[ACGT]AG
DsaI 710 CCGTGG C^C[GA][CT]GG
EcoNI 740 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 753 GGTCACC G^GT[ACGT]ACC
EcoT14I 590 CCAAGG C^C[AT][AT]GG
MaeII 734 ACGT A^CGT
MluNI 713 TGGCCA TGG^CCA
MscI 713 TGGCCA TGG^CCA
NaeI 442 GCCGGC GCC^GGC
NgoMIV 442 GCCGGC G^CCGGC
NspI 521 GCATGT [GA]CATG^[CT]
PpuMI 67 GGGACCC [GA]G^G[AT]CC[CT]
PstI 127 CTGCAG CTGCA^G
PvuI 763 CGATCG CGAT^CG
RsaI 114 GTAC GT^AC
SfcI 127 CTGCAG C^T[GA][CT]AG
SfiI 33 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 479 CCCGGG CCC^GGG
StuI 5 AGGCCT AGG^CCT
StyI 590 CCAAGG C^C[AT][AT]GG
XcmI 377 CCATCCGGCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 479 CCCGGG C^CCGGG
XmaI 479 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 107 CGCG CG^CG
336 CGCG
AcyI 470 GGCGCC G[GA]^CG[CT]C
726 GGCGCC
AlwI 96 GATCC GGATC(4/5)
743 GGATC
ApaI 41 GGGCCC GGGCC^C
625 GGGCCC
AvaI 479 CCCGGG C^[CT]CG[GA]G
618 CTCGGG
BanI 470 GGCGCC G^G[CT][GA]CC
726 GGCGCC
BbeI 470 GGCGCC GGCGC^C
726 GGCGCC
BpmI 678 CTCCAG CTGGAG(16/14)
322 CTGGAG
BsaHI 470 GGCGCC G[GA]^CG[CT]C
726 GGCGCC
BsgI 687 CTGCAC GTGCAG(16/14)
432 GTGCAG
BsoBI 479 CCCGGG C^[CT]CG[GA]G
618 CTCGGG
BsrFI 442 GCCGGC [GA]^CCGG[CT]
757 ACCGGC
BstUI 107 CGCG CG^CG
336 CGCG
Cfr10I 442 GCCGGC [GA]^CCGG[CT]
757 ACCGGC
Hin1I 470 GGCGCC G[GA]^CG[CT]C
726 GGCGCC
KasI 470 GGCGCC G^GCGCC
726 GGCGCC
MboII 16 TCTTC GAAGA(8/7)
698 TCTTC
MslI 516 CACCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
717 CACCGCTGTG
MvnI 107 CGCG CG^CG
336 CGCG
NarI 470 GGCGCC GG^CGCC
726 GGCGCC
PvuII 220 CAGCTG CAG^CTG
346 CAGCTG
SfoI 470 GGCGCC GGC^GCC
726 GGCGCC
Tsp45I 614 GTGAC ^GT[GC]AC
754 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BsiEI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
DraII EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI Sau3AI Sau96I ScrFI
TseI TspRI Van91I