Restriction map for
pFN21AE1863
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BbrPI BbsI BciVI BclI BfaI
BfrI BlnI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI DraI DrdI Eam1105I
EarI Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MamI
MboII MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SalI SapI ScaI
SexAI SfaNI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TfiI
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 118 GGCGCC G[GA]^CG[CT]C
AlwNI 466 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 560 AGCGCT AGC^GCT
AspHI 640 GAGCTC G[AT]GC[AT]^C
AviII 242 TGCGCA TGC^GCA
BbeI 118 GGCGCC GGCGC^C
BbvCI 1045 GCTGAGG CCTCAGC(-5/-2)
BcgI 620 CGATGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 397 GCCTGTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 881 AGATCT A^GATCT
BlpI 642 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 642 GCTCAGC GC^T[ACGT]AGC
BsaHI 118 GGCGCC G[GA]^CG[CT]C
BsiEI 263 CGGCCG CG[GA][CT]^CG
BsiHKAI 640 GAGCTC G[AT]GC[AT]^C
BsmBI 357 CGTCTC CGTCTC(1/5)
BstAPI 312 GCACACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 660 CATCC GGATG(2/0)
BstYI 881 AGATCT [GA]^GATC[CT]
CelII 642 GCTCAGC GC^T[ACGT]AGC
CpoI 155 CGGACCG CG^G[AT]CCG
DraIII 313 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 263 CGGCCG C^GGCCG
EclXI 263 CGGCCG C^GGCCG
Eco47III 560 AGCGCT AGC^GCT
Eco52I 263 CGGCCG C^GGCCG
EcoNI 1041 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 113 CCTTGG C^C[AT][AT]GG
FokI 660 CATCC GGATG(9/13)
FspI 242 TGCGCA TGC^GCA
HgaI 230 GCGTC GACGC(5/10)
Hin1I 118 GGCGCC G[GA]^CG[CT]C
KasI 118 GGCGCC G^GCGCC
MaeII 539 ACGT A^CGT
MflI 881 AGATCT [GA]^GATC[CT]
MseI 1111 TTAA T^TAA
MslI 417 CACGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1034 GCCGGC GCC^GGC
NarI 118 GGCGCC GG^CGCC
NgoMIV 1034 GCCGGC G^CCGGC
PflMI 465 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 585 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 76 CTGCAG CTGCA^G
RsrII 155 CGGACCG CG^G[AT]CCG
SacI 640 GAGCTC GAGCT^C
SbfI 75 CCTGCAGG CCTGCA^GG
SfcI 76 CTGCAG C^T[GA][CT]AG
SfiI 396 GGCCTGTACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 118 GGCGCC GGC^GCC
Sse8387I 75 CCTGCAGG CCTGCA^GG
StyI 113 CCTTGG C^C[AT][AT]GG
Tru9I 1111 TTAA T^TAA
Van91I 465 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 881 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 333 GTAC GT^AC
401 GTAC
AlwI 915 GATCC GGATC(4/5)
1133 GGATC
ApaI 492 GGGCCC GGGCC^C
895 GGGCCC
BpmI 61 CTCCAG CTGGAG(16/14)
472 CTGGAG
BstEII 382 GGTGACC G^GT[ACGT]ACC
943 GGTGACC
BstPI 382 GGTGACC G^GT[ACGT]ACC
943 GGTGACC
EcoO65I 382 GGTGACC G^GT[ACGT]ACC
943 GGTGACC
HaeII 118 GGCGCC [GA]GCGC^[CT]
560 AGCGCT
KspI 159 CCGCGG CCGC^GG
956 CCGCGG
PpuMI 268 GGGACCC [GA]G^G[AT]CC[CT]
583 GGGACCC
PvuII 349 CAGCTG CAG^CTG
949 CAGCTG
RsaI 333 GTAC GT^AC
401 GTAC
SacII 159 CCGCGG CCGC^GG
956 CCGCGG
TspRI 175 CAGTG CA[GC]TG[ACGT][ACGT]^
975 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI TseI
Tsp45I