Restriction map for
pFN21AE5347
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspHI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I
CelII ClaI CpoI DpnII DraI
DraIII DrdI Eco47III Eco81I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HindIII HpaI KasI KspI
MamI MboI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI Sau3AI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 131 GGTACC G^GTACC
AccI 283 GTATAC GT^[AC][GT]AC
AccII 640 CGCG CG^CG
AcyI 362 GGCGTC G[GA]^CG[CT]C
AhdI 587 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 328 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 131 GGTACC G^GTACC
AspEI 587 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 495 CTCGGG C^[CT]CG[GA]G
AvaII 299 GGACC G^G[AT]CC
BalI 344 TGGCCA TGG^CCA
BanI 131 GGTACC G^G[CT][GA]CC
BanII 163 GGGCTC G[GA]GC[CT]^C
BbsI 16 GTCTTC GAAGAC(2/6)
BciVI 844 GGATAC GTATCC(6/5)
BfaI 382 CTAG C^TAG
BpuAI 16 GTCTTC GAAGAC(2/6)
BsaHI 362 GGCGTC G[GA]^CG[CT]C
BsaWI 128 TCCGGT [AT]^CCGG[AT]
BsiEI 209 CGGCCG CG[GA][CT]^CG
BsoBI 495 CTCGGG C^[CT]CG[GA]G
Bsp1407I 230 TGTACA T^GTACA
BsrFI 239 ACCGGC [GA]^CCGG[CT]
BsrGI 230 TGTACA T^GTACA
Bst1107I 283 GTATAC GTA^TAC
BstEII 177 GGTCACC G^GT[ACGT]ACC
BstPI 177 GGTCACC G^GT[ACGT]ACC
BstUI 640 CGCG CG^CG
BstZ17I 283 GTATAC GTA^TAC
Cac8I 549 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 538 GCGC GCG^C
Cfr10I 239 ACCGGC [GA]^CCGG[CT]
DraII 298 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 159 CCACGG C^C[GA][CT]GG
EagI 209 CGGCCG C^GGCCG
Eam1105I 587 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 504 CTCTTC CTCTTC(1/4)
EclXI 209 CGGCCG C^GGCCG
Eco52I 209 CGGCCG C^GGCCG
EcoO109I 298 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 177 GGTCACC G^GT[ACGT]ACC
HaeII 537 GGCGCT [GA]GCGC^[CT]
HhaI 538 GCGC GCG^C
Hin1I 362 GGCGTC G[GA]^CG[CT]C
HinP1I 538 GCGC G^CGC
KpnI 131 GGTACC GGTAC^C
Ksp632I 504 CTCTTC CTCTTC(1/4)
MaeI 382 CTAG C^TAG
MluNI 344 TGGCCA TGG^CCA
MscI 344 TGGCCA TGG^CCA
MslI 636 CACCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 640 CGCG CG^CG
PpuMI 298 GGGACCT [GA]G^G[AT]CC[CT]
PstI 688 CTGCAG CTGCA^G
PvuII 433 CAGCTG CAG^CTG
SbfI 687 CCTGCAGG CCTGCA^GG
SexAI 224 ACCTGGT A^CC[AT]GGT
SfcI 688 CTGCAG C^T[GA][CT]AG
Sse8387I 687 CCTGCAGG CCTGCA^GG
SspBI 230 TGTACA T^GTACA
TaqI 29 TCGA T^CGA
TthHB8I 29 TCGA T^CGA
XcmI 831 CCACAACCACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 434 AGCT AG^CT
779 AGCT
BcnI 212 CCGGG CC^[GC]GG
408 CCGGG
BsaAI 271 TACGTG [CT]AC^GT[GA]
625 TACGTG
BsmAI 205 GTCTC GTCTC(1/5)
330 GTCTC
BsmFI 298 GGGAC GGGAC(10/14)
498 GGGAC
Cfr13I 299 GGACC G^G[ACGT]CC
371 GGCCC
DdeI 367 CTGAG C^T[ACGT]AG
729 CTCAG
EcoNI 684 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
816 CCTGGGCGAGG
HgaI 75 GACGC GACGC(5/10)
363 GCGTC
HincII 412 GTCAAC GT[CT]^[GA]AC
810 GTTGAC
HindII 412 GTCAAC GT[CT]^[GA]AC
810 GTTGAC
HinfI 121 GACTC G^A[ACGT]TC
557 GACTC
MspA1I 4 CAGCGG C[AC]G^C[GT]G
433 CAGCTG
NciI 212 CCGGG CC^[GC]GG
408 CCGGG
PleI 121 GACTC GAGTC(4/5)
557 GACTC
Sau96I 299 GGACC G^G[ACGT]CC
371 GGCCC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI BbvI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeII MaeIII MboII MnlI MspI
MvaI MwoI NlaIII NlaIV RsaI
ScrFI TseI Tsp45I TspRI