Restriction map for
pFN21AE9554
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BciVI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BsrI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MflI MluI MroI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 924 AAATTT [GA]^AATT[CT]
AflIII 698 ACATGT A^C[GA][CT]GT
AocI 435 CCTCAGG CC^T[ACGT]AGG
ApoI 924 AAATTT [GA]^AATT[CT]
AvaI 67 CCCGGG C^[CT]CG[GA]G
BalI 404 TGGCCA TGG^CCA
BanI 310 GGCGCC G^G[CT][GA]CC
BbeI 310 GGCGCC GGCGC^C
BbsI 616 GTCTTC GAAGAC(2/6)
BbvCI 21 CCTCAGC CCTCAGC(-5/-2)
BclI 161 TGATCA T^GATCA
BfaI 157 CTAG C^TAG
BglI 238 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 616 GTCTTC GAAGAC(2/6)
BsgI 734 CTGCAC GTGCAG(16/14)
BsiEI 11 CGGTCG CG[GA][CT]^CG
BsmFI 444 GTCCC GGGAC(10/14)
BsoBI 67 CCCGGG C^[CT]CG[GA]G
BspLU11I 698 ACATGT A^CATGT
BsrBI 634 CCGCTC CCGCTC(-3/-3)
BsrDI 489 CATTGC GCAATG(2/0)
BsrFI 834 GCCGGC [GA]^CCGG[CT]
BstXI 488 CCATTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 435 CCTCAGG CC^T[ACGT]AGG
Cfr10I 834 GCCGGC [GA]^CCGG[CT]
Eco81I 435 CCTCAGG CC^T[ACGT]AGG
EcoT22I 908 ATGCAT ATGCA^T
FbaI 161 TGATCA T^GATCA
HgaI 831 GACGC GACGC(5/10)
KasI 310 GGCGCC G^GCGCC
KspI 196 CCGCGG CCGC^GG
MaeI 157 CTAG C^TAG
MfeI 1032 CAATTG C^AATTG
MluNI 404 TGGCCA TGG^CCA
MscI 404 TGGCCA TGG^CCA
MseI 671 TTAA T^TAA
MunI 1032 CAATTG C^AATTG
NaeI 834 GCCGGC GCC^GGC
NarI 310 GGCGCC GG^CGCC
NcoI 494 CCATGG C^CATGG
NgoMIV 834 GCCGGC G^CCGGC
NsiI 908 ATGCAT ATGCA^T
NspI 698 ACATGT [GA]CATG^[CT]
PflMI 864 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 665 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 196 CCGCGG CCGC^GG
SapI 255 GCTCTTC GCTCTTC(1/4)
SfoI 310 GGCGCC GGC^GCC
SmaI 67 CCCGGG CCC^GGG
TaqI 1008 TCGA T^CGA
Tru9I 671 TTAA T^TAA
TthHB8I 1008 TCGA T^CGA
Van91I 864 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 296 CCACCTGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 67 CCCGGG C^CCGGG
XmaI 67 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 310 GGCGCC G[GA]^CG[CT]C
831 GACGCC
AluI 5 AGCT AG^CT
40 AGCT
Alw44I 412 GTGCAC G^TGCAC
606 GTGCAC
ApaLI 412 GTGCAC G^TGCAC
606 GTGCAC
AvaII 393 GGACC G^G[AT]CC
666 GGTCC
BanII 49 GAGCCC G[GA]GC[CT]^C
64 GAGCCC
BsaAI 400 TACGTG [CT]AC^GT[GA]
598 TACGTG
BsaHI 310 GGCGCC G[GA]^CG[CT]C
831 GACGCC
BseRI 426 CTCCTC GAGGAG(10/8)
429 CTCCTC
BspMI 251 ACCTGC ACCTGC(4/8)
626 ACCTGC
BssHII 175 GCGCGC G^CGCGC
537 GCGCGC
DpnII 126 GATC ^GATC
162 GATC
DraII 521 AGGGCCT [GA]G^G[ACGT]CC[CT]
665 AGGTCCT
EcoNI 21 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
431 CCTCCCTCAGG
EcoO109I 521 AGGGCCT [GA]G^G[ACGT]CC[CT]
665 AGGTCCT
EcoT14I 494 CCATGG C^C[AT][AT]GG
662 CCAAGG
Hin1I 310 GGCGCC G[GA]^CG[CT]C
831 GACGCC
MaeII 401 ACGT A^CGT
599 ACGT
MaeIII 103 GTCAC ^GT[ACGT]AC
959 GTCAC
MboI 126 GATC ^GATC
162 GATC
NdeII 126 GATC ^GATC
162 GATC
Sau3AI 126 GATC ^GATC
162 GATC
StuI 593 AGGCCT AGG^CCT
935 AGGCCT
StyI 494 CCATGG C^C[AT][AT]GG
662 CCAAGG
Tsp45I 103 GTCAC ^GT[GC]AC
959 GTCAC
TspRI 568 CAGTG CA[GC]TG[ACGT][ACGT]^
865 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AspHI BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DsaI EaeI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI TfiI TseI Tsp509I