################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeII MamI MflI MluI MluNI MroI MscI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Tth111I Van91I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcyI 13 GGCGCC G[GA]^CG[CT]C AlwNI 1010 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 442 ATTAAT AT^TAAT AsnI 442 ATTAAT AT^TAAT Asp700I 1084 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 192 GAGCTC G[GA]GC[CT]^C BbeI 13 GGCGCC GGCGC^C BsaHI 13 GGCGCC G[GA]^CG[CT]C BsmAI 184 GAGAC GTCTC(1/5) BsmBI 184 GAGACG CGTCTC(1/5) Bsp1407I 630 TGTACA T^GTACA BspDI 754 ATCGAT AT^CGAT BspHI 1080 TCATGA T^CATGA BsrDI 555 GCAATG GCAATG(2/0) BsrGI 630 TGTACA T^GTACA BstXI 905 CCATGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 684 GCAGGC GC[ACGT]^[ACGT]GC CfoI 14 GCGC GCG^C ClaI 754 ATCGAT AT^CGAT DraI 547 TTTAAA TTT^AAA DraII 130 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 905 CCATGG C^C[GA][CT]GG EcoO109I 130 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 905 CCATGG C^C[AT][AT]GG EcoT22I 386 ATGCAT ATGCA^T HaeII 13 GGCGCC [GA]GCGC^[CT] HhaI 14 GCGC GCG^C Hin1I 13 GGCGCC G[GA]^CG[CT]C HinP1I 14 GCGC G^CGC KasI 13 GGCGCC G^GCGCC MfeI 110 CAATTG C^AATTG MspA1I 1037 CAGCTG C[AC]G^C[GT]G MunI 110 CAATTG C^AATTG NarI 13 GGCGCC GG^CGCC NcoI 905 CCATGG C^CATGG NdeI 823 CATATG CA^TATG NsiI 386 ATGCAT ATGCA^T NspI 728 GCATGT [GA]CATG^[CT] PpuMI 130 GGGACCC [GA]G^G[AT]CC[CT] PshBI 442 ATTAAT AT^TAAT PstI 1034 CTGCAG CTGCA^G PvuII 1037 CAGCTG CAG^CTG RcaI 1080 TCATGA T^CATGA SacI 192 GAGCTC GAGCT^C SapI 189 GAAGAGC GCTCTTC(1/4) SfcI 1034 CTGCAG C^T[GA][CT]AG SfoI 13 GGCGCC GGC^GCC SmlI 195 CTCAAG C^T[CT][GA]AG SpeI 811 ACTAGT A^CTAGT SspBI 630 TGTACA T^GTACA SspI 377 AATATT AAT^ATT StyI 905 CCATGG C^C[AT][AT]GG Tsp45I 1063 GTCAC ^GT[GC]AC XbaI 885 TCTAGA T^CTAGA XcmI 518 CCAAGCTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1084 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 598 AAATTT [GA]^AATT[CT] 692 AAATTC ApoI 598 AAATTT [GA]^AATT[CT] 692 AAATTC AvaI 134 CCCGAG C^[CT]CG[GA]G 948 CTCGGG BanI 13 GGCGCC G^G[CT][GA]CC 1024 GGCACC BcnI 857 CCCGG CC^[GC]GG 968 CCGGG BpmI 673 CTGGAG CTGGAG(16/14) 763 CTGGAG BseRI 137 GAGGAG GAGGAG(10/8) 160 GAGGAG BsgI 847 CTGCAC GTGCAG(16/14) 991 CTGCAC BsiYI 153 CCAGTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 858 CCGGTTTGAGG BslI 153 CCAGTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 858 CCGGTTTGAGG BsmFI 130 GGGAC GGGAC(10/14) 560 GGGAC BsoBI 134 CCCGAG C^[CT]CG[GA]G 948 CTCGGG BsrI 1045 ACTGG ACTGG(1/-1) 153 CCAGT DpnII 40 GATC ^GATC 883 GATC HaeIII 687 GGCC GG^CC 760 GGCC HapII 858 CCGG C^CGG 968 CCGG HpaII 858 CCGG C^CGG 968 CCGG MboI 40 GATC ^GATC 883 GATC MspI 858 CCGG C^CGG 968 CCGG NciI 857 CCCGG CC^[GC]GG 968 CCGGG NdeII 40 GATC ^GATC 883 GATC Sau3AI 40 GATC ^GATC 883 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BbvI BfaI BmyI BsaJI BsiHKAI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MslI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I