Restriction map for
pFN21AB6540
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeII MamI
MflI MluI MluNI MroI MscI
MvnI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI Tth111I
Van91I XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcyI 13 GGCGCC G[GA]^CG[CT]C
AlwNI 1010 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 442 ATTAAT AT^TAAT
AsnI 442 ATTAAT AT^TAAT
Asp700I 1084 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 192 GAGCTC G[GA]GC[CT]^C
BbeI 13 GGCGCC GGCGC^C
BsaHI 13 GGCGCC G[GA]^CG[CT]C
BsmAI 184 GAGAC GTCTC(1/5)
BsmBI 184 GAGACG CGTCTC(1/5)
Bsp1407I 630 TGTACA T^GTACA
BspDI 754 ATCGAT AT^CGAT
BspHI 1080 TCATGA T^CATGA
BsrDI 555 GCAATG GCAATG(2/0)
BsrGI 630 TGTACA T^GTACA
BstXI 905 CCATGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 684 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 14 GCGC GCG^C
ClaI 754 ATCGAT AT^CGAT
DraI 547 TTTAAA TTT^AAA
DraII 130 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 905 CCATGG C^C[GA][CT]GG
EcoO109I 130 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 905 CCATGG C^C[AT][AT]GG
EcoT22I 386 ATGCAT ATGCA^T
HaeII 13 GGCGCC [GA]GCGC^[CT]
HhaI 14 GCGC GCG^C
Hin1I 13 GGCGCC G[GA]^CG[CT]C
HinP1I 14 GCGC G^CGC
KasI 13 GGCGCC G^GCGCC
MfeI 110 CAATTG C^AATTG
MspA1I 1037 CAGCTG C[AC]G^C[GT]G
MunI 110 CAATTG C^AATTG
NarI 13 GGCGCC GG^CGCC
NcoI 905 CCATGG C^CATGG
NdeI 823 CATATG CA^TATG
NsiI 386 ATGCAT ATGCA^T
NspI 728 GCATGT [GA]CATG^[CT]
PpuMI 130 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 442 ATTAAT AT^TAAT
PstI 1034 CTGCAG CTGCA^G
PvuII 1037 CAGCTG CAG^CTG
RcaI 1080 TCATGA T^CATGA
SacI 192 GAGCTC GAGCT^C
SapI 189 GAAGAGC GCTCTTC(1/4)
SfcI 1034 CTGCAG C^T[GA][CT]AG
SfoI 13 GGCGCC GGC^GCC
SmlI 195 CTCAAG C^T[CT][GA]AG
SpeI 811 ACTAGT A^CTAGT
SspBI 630 TGTACA T^GTACA
SspI 377 AATATT AAT^ATT
StyI 905 CCATGG C^C[AT][AT]GG
Tsp45I 1063 GTCAC ^GT[GC]AC
XbaI 885 TCTAGA T^CTAGA
XcmI 518 CCAAGCTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1084 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 598 AAATTT [GA]^AATT[CT]
692 AAATTC
ApoI 598 AAATTT [GA]^AATT[CT]
692 AAATTC
AvaI 134 CCCGAG C^[CT]CG[GA]G
948 CTCGGG
BanI 13 GGCGCC G^G[CT][GA]CC
1024 GGCACC
BcnI 857 CCCGG CC^[GC]GG
968 CCGGG
BpmI 673 CTGGAG CTGGAG(16/14)
763 CTGGAG
BseRI 137 GAGGAG GAGGAG(10/8)
160 GAGGAG
BsgI 847 CTGCAC GTGCAG(16/14)
991 CTGCAC
BsiYI 153 CCAGTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
858 CCGGTTTGAGG
BslI 153 CCAGTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
858 CCGGTTTGAGG
BsmFI 130 GGGAC GGGAC(10/14)
560 GGGAC
BsoBI 134 CCCGAG C^[CT]CG[GA]G
948 CTCGGG
BsrI 1045 ACTGG ACTGG(1/-1)
153 CCAGT
DpnII 40 GATC ^GATC
883 GATC
HaeIII 687 GGCC GG^CC
760 GGCC
HapII 858 CCGG C^CGG
968 CCGG
HpaII 858 CCGG C^CGG
968 CCGG
MboI 40 GATC ^GATC
883 GATC
MspI 858 CCGG C^CGG
968 CCGG
NciI 857 CCCGG CC^[GC]GG
968 CCGGG
NdeII 40 GATC ^GATC
883 GATC
Sau3AI 40 GATC ^GATC
883 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BfaI BmyI BsaJI BsiHKAI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI EarI EcoRII Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboII MnlI MseI MslI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I