##################################
# NO CUT ENZYMES (  221 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoT14I     EcoT22I     FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HaeIII      
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HphI        ItaI        KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MunI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AluI           59  AGCT                 AG^CT
BpmI           50  CTCCAG               CTGGAG(16/14)
BsaJI           4  CCTGGG               C^C[ACGT][ACGT]GG
BsiYI          39  CCCCGCGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           39  CCCCGCGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          98  GAGAC                GTCTC(1/5)
BssHII         16  GCGCGC               G^CGCGC
BssKI           4  CCTGG                ^CC[ACGT]GG
BstNI           4  CCTGG                CC^[AT]GG
EarI          145  CTCTTC               CTCTTC(1/4)
EcoRII          4  CCTGG                ^CC[AT]GG
EcoRV         135  GATATC               GAT^ATC
HapII         114  CCGG                 C^CGG
HinfI         129  GAATC                G^A[ACGT]TC
HpaII         114  CCGG                 C^CGG
Ksp632I       145  CTCTTC               CTCTTC(1/4)
MspI          114  CCGG                 C^CGG
MvaI            4  CCTGG                CC^[AT]GG
ScrFI           4  CCTGG                CC^[ACGT]GG
TaqI           68  TCGA                 T^CGA
TfiI          129  GAATC                G^A[AT]TC
TthHB8I        68  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AciI           41  CCGC                 CCGC(-3/-1)
               45  GCGG                
BanII          36  GAGCCC               G[GA]GC[CT]^C
               47  GGGCTC              
BmyI           36  GAGCCC               G[AGT]GC[ACT]^C
               47  GGGCTC              
Bsp1286I       36  GAGCCC               G[AGT]GC[ACT]^C
               47  GGGCTC              
Cac8I          16  GCGCGC               GC[ACGT]^[ACGT]GC
               45  GCGGGC              
DdeI           85  CTCAG                C^T[ACGT]AG
              153  CTCAG               
FauI           40  CCCGC                CCCGC(4/6)
               45  GCGGG               
MboII           9  GAAGA                GAAGA(8/7)
              146  TCTTC               
NlaIV          48  GGCTCC               GG[ACGT]^[ACGT]CC
              157  GGAACC              


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AccII       BstUI       CfoI        HhaI        HinP1I      
MvnI