Restriction map for
pFN21AE6270
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbvCI
BbvI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoT14I
EcoT22I FbaI Fnu4HI FokI FseI
FspI HincII HindII HindIII HpaI
ItaI KpnI KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfaNI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI TseI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 36 CGCG CG^CG
AcyI 13 GGCGCC G[GA]^CG[CT]C
AfaI 411 GTAC GT^AC
AspHI 157 GTGCTC G[AT]GC[AT]^C
AvaII 60 GGACC G^G[AT]CC
BanI 13 GGCGCC G^G[CT][GA]CC
BbeI 13 GGCGCC GGCGC^C
BcnI 252 CCGGG CC^[GC]GG
BglI 16 GCCGTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 13 GGCGCC G[GA]^CG[CT]C
BsaI 227 GAGACC GGTCTC(1/5)
BsaJI 17 CCGTGG C^C[ACGT][ACGT]GG
BseRI 224 GAGGAG GAGGAG(10/8)
BsgI 128 CTGCAC GTGCAG(16/14)
BsiHKAI 157 GTGCTC G[AT]GC[AT]^C
BsmFI 59 GGGAC GGGAC(10/14)
Bsp1407I 410 TGTACA T^GTACA
BsrFI 210 GCCGGC [GA]^CCGG[CT]
BsrGI 410 TGTACA T^GTACA
BsrI 428 ACTGG ACTGG(1/-1)
BstEII 489 GGTCACC G^GT[ACGT]ACC
BstNI 96 CCAGG CC^[AT]GG
BstPI 489 GGTCACC G^GT[ACGT]ACC
BstUI 36 CGCG CG^CG
BstXI 179 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 210 GCCGGC [GA]^CCGG[CT]
Cfr13I 60 GGACC G^G[ACGT]CC
DdeI 193 CTGAG C^T[ACGT]AG
DraII 59 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 17 CCGTGG C^C[GA][CT]GG
EarI 455 GAAGAG CTCTTC(1/4)
EcoO109I 59 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 489 GGTCACC G^GT[ACGT]ACC
EcoRII 96 CCAGG ^CC[AT]GG
EcoRV 49 GATATC GAT^ATC
HgaI 37 GCGTC GACGC(5/10)
Hin1I 13 GGCGCC G[GA]^CG[CT]C
HinfI 571 GAATC G^A[ACGT]TC
KasI 13 GGCGCC G^GCGCC
Ksp632I 455 GAAGAG CTCTTC(1/4)
MvaI 96 CCAGG CC^[AT]GG
MvnI 36 CGCG CG^CG
NaeI 210 GCCGGC GCC^GGC
NarI 13 GGCGCC GG^CGCC
NciI 252 CCGGG CC^[GC]GG
NgoMIV 210 GCCGGC G^CCGGC
PflMI 475 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 59 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 411 GTAC GT^AC
SapI 455 GAAGAGC GCTCTTC(1/4)
Sau96I 60 GGACC G^G[ACGT]CC
SexAI 95 ACCAGGT A^CC[AT]GGT
SfoI 13 GGCGCC GGC^GCC
SspBI 410 TGTACA T^GTACA
TfiI 571 GAATC G^A[AT]TC
Tsp45I 490 GTCAC ^GT[GC]AC
TspRI 168 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 475 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 35 CCGC CCGC(-3/-1)
10 GCGG
AluI 86 AGCT AG^CT
270 AGCT
BanII 23 GGGCTC G[GA]GC[CT]^C
174 GAGCCC
BbsI 256 GAAGAC GAAGAC(2/6)
123 GTCTTC
BpuAI 256 GAAGAC GAAGAC(2/6)
123 GTCTTC
BspHI 134 TCATGA T^CATGA
339 TCATGA
BssKI 96 CCAGG ^CC[ACGT]GG
252 CCGGG
CfoI 14 GCGC GCG^C
81 GCGC
DpnII 117 GATC ^GATC
153 GATC
DraI 102 TTTAAA TTT^AAA
289 TTTAAA
FauI 34 CCCGC CCCGC(4/6)
10 GCGGG
HaeII 13 GGCGCC [GA]GCGC^[CT]
80 AGCGCC
HaeIII 213 GGCC GG^CC
421 GGCC
HhaI 14 GCGC GCG^C
81 GCGC
HinP1I 14 GCGC G^CGC
81 GCGC
HphI 274 GGTGA GGTGA(8/7)
491 TCACC
MaeII 446 ACGT A^CGT
567 ACGT
MaeIII 490 GTCAC ^GT[ACGT]AC
558 GTTAC
MboI 117 GATC ^GATC
153 GATC
MseI 103 TTAA T^TAA
290 TTAA
NdeI 476 CATATG CA^TATG
551 CATATG
NdeII 117 GATC ^GATC
153 GATC
RcaI 134 TCATGA T^CATGA
339 TCATGA
Sau3AI 117 GATC ^GATC
153 GATC
ScrFI 96 CCAGG CC^[ACGT]GG
252 CCGGG
Tru9I 103 TTAA T^TAA
290 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AcsI ApoI BmyI BsiYI BslI
BsmAI Bsp1286I Cac8I HapII HpaII
MboII MnlI MslI MspI MwoI
NlaIII NlaIV Tsp509I