Restriction map for
pFN21AB6975
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwNI 498 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 343 GAGCTC G[AT]GC[AT]^C
AvaI 30 CCCGGG C^[CT]CG[GA]G
BalI 228 TGGCCA TGG^CCA
BanII 343 GAGCTC G[GA]GC[CT]^C
BmyI 343 GAGCTC G[AGT]GC[ACT]^C
BsaI 481 GAGACC GGTCTC(1/5)
BsiHKAI 343 GAGCTC G[AT]GC[AT]^C
BsmAI 481 GAGAC GTCTC(1/5)
BsoBI 30 CCCGGG C^[CT]CG[GA]G
Bsp1286I 343 GAGCTC G[AGT]GC[ACT]^C
BspHI 144 TCATGA T^CATGA
BsrDI 103 GCAATG GCAATG(2/0)
BstAPI 258 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 563 CATCC GGATG(2/0)
DpnII 406 GATC ^GATC
DraI 337 TTTAAA TTT^AAA
EaeI 228 TGGCCA [CT]^GGCC[GA]
EcoRI 238 GAATTC G^AATTC
FokI 563 CATCC GGATG(9/13)
HaeIII 229 GGCC GG^CC
HapII 31 CCGG C^CGG
HpaII 31 CCGG C^CGG
MboI 406 GATC ^GATC
MluNI 228 TGGCCA TGG^CCA
MscI 228 TGGCCA TGG^CCA
MseI 338 TTAA T^TAA
MspA1I 21 CAGCTG C[AC]G^C[GT]G
MspI 31 CCGG C^CGG
NdeII 406 GATC ^GATC
NlaIV 49 GGGTCC GG[ACGT]^[ACGT]CC
NspI 263 ACATGC [GA]CATG^[CT]
PshAI 174 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 488 CTGCAG CTGCA^G
PvuII 21 CAGCTG CAG^CTG
RcaI 144 TCATGA T^CATGA
SacI 343 GAGCTC GAGCT^C
Sau3AI 406 GATC ^GATC
SmaI 30 CCCGGG CCC^GGG
SmlI 444 CTTGAG C^T[CT][GA]AG
TfiI 500 GATTC G^A[AT]TC
Tru9I 338 TTAA T^TAA
Tsp45I 301 GTCAC ^GT[GC]AC
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 60 GTAC GT^AC
261 GTAC
AvaII 50 GGTCC G^G[AT]CC
357 GGTCC
BcnI 30 CCCGG CC^[GC]GG
31 CCGGG
BsiYI 176 CCCAGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
381 CCTGGTGCTGG
BslI 176 CCCAGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
381 CCTGGTGCTGG
CfoI 38 GCGC GCG^C
101 GCGC
Cfr13I 50 GGTCC G^G[ACGT]CC
357 GGTCC
DdeI 496 CTCAG C^T[ACGT]AG
528 CTTAG
EarI 585 GAAGAG CTCTTC(1/4)
633 GAAGAG
FauI 47 GCGGG CCCGC(4/6)
252 GCGGG
HhaI 38 GCGC GCG^C
101 GCGC
HinP1I 38 GCGC G^CGC
101 GCGC
Ksp632I 585 GAAGAG CTCTTC(1/4)
633 GAAGAG
MaeIII 301 GTCAC ^GT[ACGT]AC
395 GTAAC
MslI 243 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
364 CACACCTATG
NciI 30 CCCGG CC^[GC]GG
31 CCGGG
PleI 447 GAGTC GAGTC(4/5)
553 GAGTC
RsaI 60 GTAC GT^AC
261 GTAC
Sau96I 50 GGTCC G^G[ACGT]CC
357 GGTCC
TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^
303 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BssKI BstNI Cac8I EcoRII
Fnu4HI HinfI ItaI MboII MnlI
MvaI MwoI NlaIII ScrFI SfaNI
SfcI TseI Tsp509I