Restriction map for
pFN21AB5781
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AviII AvrII BalI BbeI BbsI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
Sse8387I SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 29 ACGTGT A^C[GA][CT]GT
ApaI 79 GGGCCC GGGCC^C
AseI 1339 ATTAAT AT^TAAT
AsnI 1339 ATTAAT AT^TAAT
AspI 1177 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 176 CCCGAG C^[CT]CG[GA]G
BamHI 1188 GGATCC G^GATCC
BanI 6 GGCACC G^G[CT][GA]CC
BbrPI 28 CACGTG CAC^GTG
BbvCI 1748 CCTCAGC CCTCAGC(-5/-2)
BcgI 178 CGAGTCAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1932 GGATAC GTATCC(6/5)
BcnI 48 CCGGG CC^[GC]GG
BglII 660 AGATCT A^GATCT
BsmFI 1495 GTCCC GGGAC(10/14)
BsoBI 176 CCCGAG C^[CT]CG[GA]G
Bsp1407I 379 TGTACA T^GTACA
BspHI 843 TCATGA T^CATGA
BspMI 1599 GCAGGT ACCTGC(4/8)
BsrFI 838 GCCGGT [GA]^CCGG[CT]
BsrGI 379 TGTACA T^GTACA
BstXI 1123 CCAAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 838 GCCGGT [GA]^CCGG[CT]
DraII 321 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2440 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 321 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 557 GAATTC G^AATTC
FauI 54 GCGGG CCCGC(4/6)
HgaI 2180 GCGTC GACGC(5/10)
MfeI 183 CAATTG C^AATTG
MunI 183 CAATTG C^AATTG
NciI 48 CCGGG CC^[GC]GG
NheI 834 GCTAGC G^CTAGC
PflFI 1177 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 28 CACGTG CAC^GTG
PmlI 28 CACGTG CAC^GTG
PpuMI 321 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1339 ATTAAT AT^TAAT
RcaI 843 TCATGA T^CATGA
SacI 72 GAGCTC GAGCT^C
ScaI 1579 AGTACT AGT^ACT
SexAI 1909 ACCTGGT A^CC[AT]GGT
SfaNI 303 GCATC GCATC(5/9)
SpeI 1576 ACTAGT A^CTAGT
SspBI 379 TGTACA T^GTACA
StuI 1297 AGGCCT AGG^CCT
Tth111I 1177 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 83 CCAGAGGACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 1999 GTGCAC G^TGCAC
2471 GTGCAC
ApaLI 1999 GTGCAC G^TGCAC
2471 GTGCAC
Asp700I 988 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1372 GAAAAAGTTC
BsaAI 28 CACGTG [CT]AC^GT[GA]
2212 TACGTG
BsaBI 917 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1468 GATAGTGATC
BsgI 1394 CTGCAC GTGCAG(16/14)
1819 GTGCAG
BstF5I 210 GGATG GGATG(2/0)
744 GGATG
DraI 291 TTTAAA TTT^AAA
462 TTTAAA
EcoT14I 932 CCATGG C^C[AT][AT]GG
1249 CCATGG
FokI 210 GGATG GGATG(9/13)
744 GGATG
HapII 48 CCGG C^CGG
839 CCGG
HpaI 1117 GTTAAC GTT^AAC
1572 GTTAAC
HpaII 48 CCGG C^CGG
839 CCGG
MamI 917 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1468 GATAGTGATC
MspA1I 398 CAGCTG C[AC]G^C[GT]G
2171 CAGCTG
MspI 48 CCGG C^CGG
839 CCGG
NcoI 932 CCATGG C^CATGG
1249 CCATGG
PshAI 419 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2169 GACAGCTGTC
PvuII 398 CAGCTG CAG^CTG
2171 CAGCTG
SphI 339 GCATGC GCATG^C
1776 GCATGC
SspI 528 AATATT AAT^ATT
1707 AATATT
StyI 932 CCATGG C^C[AT][AT]GG
1249 CCATGG
XmnI 988 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1372 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbvI BclI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstAPI BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoRII
FbaI Fnu4HI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII