Restriction map for
pFN21AE4025
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HgaI HincII HindII HindIII
HpaI HphI KpnI KspI MaeI
MfeI MflI MluI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 143 TCCGGA T^CCGGA
AcyI 36 GGCGCC G[GA]^CG[CT]C
AfaI 363 GTAC GT^AC
AgeI 656 ACCGGT A^CCGGT
AlwI 462 GGATC GGATC(4/5)
AlwNI 254 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 59 GGGCCC GGGCC^C
Asp700I 244 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 15 GAGCAC G[AT]GC[AT]^C
BalI 156 TGGCCA TGG^CCA
BanI 36 GGCGCC G^G[CT][GA]CC
BbeI 36 GGCGCC GGCGC^C
BbsI 448 GAAGAC GAAGAC(2/6)
BlpI 12 GCTGAGC GC^T[ACGT]AGC
BpmI 64 CTGGAG CTGGAG(16/14)
Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC
BpuAI 448 GAAGAC GAAGAC(2/6)
BsaAI 160 CACGTA [CT]AC^GT[GA]
BsaBI 532 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 36 GGCGCC G[GA]^CG[CT]C
BseAI 143 TCCGGA T^CCGGA
BseRI 397 GAGGAG GAGGAG(10/8)
BsiHKAI 15 GAGCAC G[AT]GC[AT]^C
BsmAI 400 GAGAC GTCTC(1/5)
BspEI 143 TCCGGA T^CCGGA
BsrFI 656 ACCGGT [GA]^CCGG[CT]
BsrI 188 CCAGT ACTGG(1/-1)
CelII 12 GCTGAGC GC^T[ACGT]AGC
Cfr10I 656 ACCGGT [GA]^CCGG[CT]
EaeI 156 TGGCCA [CT]^GGCC[GA]
EarI 644 GAAGAG CTCTTC(1/4)
EcoNI 238 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 36 GGCGCC [GA]GCGC^[CT]
Hin1I 36 GGCGCC G[GA]^CG[CT]C
KasI 36 GGCGCC G^GCGCC
Ksp632I 644 GAAGAG CTCTTC(1/4)
MamI 532 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 156 TGGCCA TGG^CCA
MroI 143 TCCGGA T^CCGGA
MscI 156 TGGCCA TGG^CCA
NarI 36 GGCGCC GG^CGCC
PinAI 656 ACCGGT A^CCGGT
PleI 647 GAGTC GAGTC(4/5)
PpuMI 77 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 363 GTAC GT^AC
SfoI 36 GGCGCC GGC^GCC
StuI 496 AGGCCT AGG^CCT
Tsp509I 624 AATT ^AATT
XmnI 244 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 54 CGCG CG^CG
56 CGCG
BanII 3 GGGCTC G[GA]GC[CT]^C
59 GGGCCC
BbvI 406 GCAGC GCAGC(8/12)
545 GCAGC
BcnI 81 CCCGG CC^[GC]GG
225 CCGGG
BsaJI 62 CCCTGG C^C[ACGT][ACGT]GG
314 CCCTGG
BsaWI 143 TCCGGA [AT]^CCGG[AT]
656 ACCGGT
BsiYI 50 CCACCGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
238 CCTTCAGAAGG
BslI 50 CCACCGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
238 CCTTCAGAAGG
BsmFI 77 GGGAC GGGAC(10/14)
422 GTCCC
BstNI 63 CCTGG CC^[AT]GG
315 CCTGG
BstUI 54 CGCG CG^CG
56 CGCG
DpnII 463 GATC ^GATC
485 GATC
DraII 59 GGGCCCT [GA]G^G[ACGT]CC[CT]
77 GGGACCC
EcoO109I 59 GGGCCCT [GA]G^G[ACGT]CC[CT]
77 GGGACCC
EcoRII 63 CCTGG ^CC[AT]GG
315 CCTGG
MaeII 97 ACGT A^CGT
161 ACGT
MboI 463 GATC ^GATC
485 GATC
MspA1I 24 CAGCGG C[AC]G^C[GT]G
150 CCGCTG
MvaI 63 CCTGG CC^[AT]GG
315 CCTGG
MvnI 54 CGCG CG^CG
56 CGCG
NciI 81 CCCGG CC^[GC]GG
225 CCGGG
NdeII 463 GATC ^GATC
485 GATC
Sau3AI 463 GATC ^GATC
485 GATC
SmlI 121 CTTGAG C^T[CT][GA]AG
394 CTTGAG
TaqI 104 TCGA T^CGA
685 TCGA
TfiI 537 GAATC G^A[AT]TC
678 GAATC
TseI 406 GCAGC G^C[AT]GC
545 GCAGC
TspRI 327 CAGTG CA[GC]TG[ACGT][ACGT]^
552 CAGTG
TthHB8I 104 TCGA T^CGA
685 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AvaII BmyI Bsp1286I
BssKI Cac8I CfoI Cfr13I DdeI
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboII MnlI MseI MspI MwoI
NlaIV Sau96I ScrFI Tru9I Tsp45I