Restriction map for
pFN21AE2542
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssSI BstAPI BstBI
Bsu36I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FseI FspI
HgaI HindIII KasI KpnI KspI
MfeI MluI MroI MunI NarI
NheI NotI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspBI StuI SwaI XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2160 GACGTC GACGT^C
AcyI 2160 GACGTC G[GA]^CG[CT]C
AlwNI 1370 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1401 GGGCCC GGGCC^C
AscI 392 GGCGCGCC GG^CGCGCC
AseI 1882 ATTAAT AT^TAAT
AsnI 1882 ATTAAT AT^TAAT
AspHI 1359 GAGCAC G[AT]GC[AT]^C
AspI 2554 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2400 TGGCCA TGG^CCA
BcnI 2391 CCGGG CC^[GC]GG
BglI 14 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2530 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2160 GACGTC G[GA]^CG[CT]C
BseRI 2063 GAGGAG GAGGAG(10/8)
BsgI 2028 CTGCAC GTGCAG(16/14)
BsiHKAI 1359 GAGCAC G[AT]GC[AT]^C
BspMI 858 GCAGGT ACCTGC(4/8)
BsrFI 44 GCCGGC [GA]^CCGG[CT]
BssHII 393 GCGCGC G^CGCGC
Bst1107I 660 GTATAC GTA^TAC
BstEII 1551 GGTCACC G^GT[ACGT]ACC
BstPI 1551 GGTCACC G^GT[ACGT]ACC
BstYI 2043 AGATCC [GA]^GATC[CT]
BstZ17I 660 GTATAC GTA^TAC
Cfr10I 44 GCCGGC [GA]^CCGG[CT]
EaeI 2400 TGGCCA [CT]^GGCC[GA]
EcoNI 465 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1551 GGTCACC G^GT[ACGT]ACC
FauI 11 CCCGC CCCGC(4/6)
HaeII 2284 GGCGCT [GA]GCGC^[CT]
Hin1I 2160 GACGTC G[GA]^CG[CT]C
HpaI 154 GTTAAC GTT^AAC
MamI 2530 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2043 AGATCC [GA]^GATC[CT]
MluNI 2400 TGGCCA TGG^CCA
MscI 2400 TGGCCA TGG^CCA
MspA1I 176 CAGCTG C[AC]G^C[GT]G
NaeI 44 GCCGGC GCC^GGC
NciI 2391 CCGGG CC^[GC]GG
NcoI 1809 CCATGG C^CATGG
NgoMIV 44 GCCGGC G^CCGGC
NruI 65 TCGCGA TCG^CGA
PflFI 2554 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2419 GACTC GAGTC(4/5)
PshAI 1825 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1882 ATTAAT AT^TAAT
PvuII 176 CAGCTG CAG^CTG
SbfI 2423 CCTGCAGG CCTGCA^GG
ScaI 842 AGTACT AGT^ACT
Sse8387I 2423 CCTGCAGG CCTGCA^GG
Tth111I 2554 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 2043 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 660 GTATAC GT^[AC][GT]AC
1001 GTCTAC
AccII 66 CGCG CG^CG
394 CGCG
AflIII 111 ACATGT A^C[GA][CT]GT
1672 ACATGT
BanI 861 GGTGCC G^G[CT][GA]CC
2308 GGCACC
BclI 908 TGATCA T^GATCA
1745 TGATCA
BsaI 1612 GAGACC GGTCTC(1/5)
2344 GAGACC
BspLU11I 111 ACATGT A^CATGT
1672 ACATGT
BstUI 66 CGCG CG^CG
394 CGCG
BstXI 1540 CCAACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2348 CCATCGAAATGG
DraI 1185 TTTAAA TTT^AAA
1210 TTTAAA
DraIII 2000 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2033 CACATCGTG
DsaI 1809 CCATGG C^C[GA][CT]GG
2312 CCGTGG
EcoT22I 889 ATGCAT ATGCA^T
2691 ATGCAT
FbaI 908 TGATCA T^GATCA
1745 TGATCA
HincII 154 GTTAAC GT[CT]^[GA]AC
532 GTTGAC
HindII 154 GTTAAC GT[CT]^[GA]AC
532 GTTGAC
MvnI 66 CGCG CG^CG
394 CGCG
NdeI 1484 CATATG CA^TATG
1934 CATATG
NsiI 889 ATGCAT ATGCA^T
2691 ATGCAT
PstI 2424 CTGCAG CTGCA^G
2454 CTGCAG
SphI 2544 GCATGC GCATG^C
2689 GCATGC
TaqI 427 TCGA T^CGA
2351 TCGA
TthHB8I 427 TCGA T^CGA
2351 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XmnI