Restriction map for
pFN21AE3981
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspHI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MseI MspA1I
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tru9I Tth111I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 673 GTAGAC GT^[AC][GT]AC
AccIII 746 TCCGGA T^CCGGA
AcsI 264 GAATTT [GA]^AATT[CT]
AcyI 723 GACGCC G[GA]^CG[CT]C
AflIII 116 ACATGT A^C[GA][CT]GT
AluI 347 AGCT AG^CT
ApoI 264 GAATTT [GA]^AATT[CT]
Asp700I 496 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 619 CTCGAG C^[CT]CG[GA]G
AvaII 615 GGTCC G^G[AT]CC
BalI 134 TGGCCA TGG^CCA
BbsI 170 GAAGAC GAAGAC(2/6)
BclI 14 TGATCA T^GATCA
BfaI 20 CTAG C^TAG
BlpI 468 GCTAAGC GC^T[ACGT]AGC
BpmI 62 CTCCAG CTGGAG(16/14)
Bpu1102I 468 GCTAAGC GC^T[ACGT]AGC
BpuAI 170 GAAGAC GAAGAC(2/6)
BsaHI 723 GACGCC G[GA]^CG[CT]C
BsaWI 746 TCCGGA [AT]^CCGG[AT]
BseAI 746 TCCGGA T^CCGGA
BsmAI 209 GTCTC GTCTC(1/5)
BsmFI 681 GTCCC GGGAC(10/14)
BsoBI 619 CTCGAG C^[CT]CG[GA]G
Bsp1407I 119 TGTACA T^GTACA
BspEI 746 TCCGGA T^CCGGA
BspLU11I 116 ACATGT A^CATGT
BsrGI 119 TGTACA T^GTACA
BstYI 22 AGATCC [GA]^GATC[CT]
Cac8I 541 GCAGGC GC[ACGT]^[ACGT]GC
CelII 468 GCTAAGC GC^T[ACGT]AGC
EarI 328 GAAGAG CTCTTC(1/4)
EcoT14I 48 CCAAGG C^C[AT][AT]GG
FauI 708 GCGGG CCCGC(4/6)
FbaI 14 TGATCA T^GATCA
HapII 747 CCGG C^CGG
Hin1I 723 GACGCC G[GA]^CG[CT]C
HpaII 747 CCGG C^CGG
Ksp632I 328 GAAGAG CTCTTC(1/4)
MaeI 20 CTAG C^TAG
MflI 22 AGATCC [GA]^GATC[CT]
MluNI 134 TGGCCA TGG^CCA
MroI 746 TCCGGA T^CCGGA
MscI 134 TGGCCA TGG^CCA
MspI 747 CCGG C^CGG
NspI 116 ACATGT [GA]CATG^[CT]
PaeR7I 619 CTCGAG C^TCGAG
PflMI 580 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 614 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 561 GACGCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI 619 CTCGAG C^T[CT][GA]AG
SspBI 119 TGTACA T^GTACA
SspI 338 AATATT AAT^ATT
StyI 48 CCAAGG C^C[AT][AT]GG
Tsp509I 265 AATT ^AATT
TspRI 38 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 580 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 619 CTCGAG C^TCGAG
XhoII 22 AGATCC [GA]^GATC[CT]
XmnI 496 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 23 GATCC GGATC(4/5)
461 GGATC
BanII 190 GAGCCC G[GA]GC[CT]^C
654 GGGCTC
BbvI 128 GCAGC GCAGC(8/12)
630 GCAGC
BmyI 190 GAGCCC G[AGT]GC[ACT]^C
654 GGGCTC
BsaJI 48 CCAAGG C^C[ACGT][ACGT]GG
450 CCTCGG
BseRI 286 CTCCTC GAGGAG(10/8)
668 GAGGAG
Bsp1286I 190 GAGCCC G[AGT]GC[ACT]^C
654 GGGCTC
BsrI 397 ACTGG ACTGG(1/-1)
684 CCAGT
BssKI 132 CCTGG ^CC[ACGT]GG
612 CCAGG
BstNI 132 CCTGG CC^[AT]GG
612 CCAGG
DraII 412 AGGCCCT [GA]G^G[ACGT]CC[CT]
614 AGGTCCT
EaeI 111 CGGCCA [CT]^GGCC[GA]
134 TGGCCA
EcoO109I 412 AGGCCCT [GA]G^G[ACGT]CC[CT]
614 AGGTCCT
EcoRII 132 CCTGG ^CC[AT]GG
612 CCAGG
HgaI 561 GACGC GACGC(5/10)
723 GACGC
MslI 97 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
99 CATTGATGTG
MvaI 132 CCTGG CC^[AT]GG
612 CCAGG
NlaIII 117 CATG CATG^
357 CATG
NlaIV 441 GGCCCC GG[ACGT]^[ACGT]CC
655 GGCTCC
PleI 556 GACTC GAGTC(4/5)
676 GACTC
ScrFI 132 CCTGG CC^[ACGT]GG
612 CCAGG
SfaNI 31 GCATC GCATC(5/9)
53 GCATC
TseI 128 GCAGC G^C[AT]GC
630 GCAGC
Tsp45I 476 GTGAC ^GT[GC]AC
596 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI BsiYI BslI BstF5I
BstXI Cfr13I DdeI DpnII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
NdeII RsaI Sau3AI Sau96I TaqI
TfiI TthHB8I