################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 1191 GGTACC G^GTACC Alw44I 1375 GTGCAC G^TGCAC ApaLI 1375 GTGCAC G^TGCAC Asp718I 1191 GGTACC G^GTACC AvaI 52 CTCGAG C^[CT]CG[GA]G BglII 168 AGATCT A^GATCT BlpI 517 GCTCAGC GC^T[ACGT]AGC BpmI 1424 CTCCAG CTGGAG(16/14) Bpu1102I 517 GCTCAGC GC^T[ACGT]AGC BsaJI 1325 CCCAGG C^C[ACGT][ACGT]GG BsmI 1110 GAATGC GAATGC(1/-1) BsoBI 52 CTCGAG C^[CT]CG[GA]G BssSI 1019 CACGAG CACGAG(-5/-1) BstAPI 1213 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 168 AGATCT [GA]^GATC[CT] CelII 517 GCTCAGC GC^T[ACGT]AGC Cfr13I 1408 GGCCC G^G[ACGT]CC DraI 246 TTTAAA TTT^AAA HincII 864 GTCAAC GT[CT]^[GA]AC HindII 864 GTCAAC GT[CT]^[GA]AC KpnI 1191 GGTACC GGTAC^C MaeII 500 ACGT A^CGT MflI 168 AGATCT [GA]^GATC[CT] NspI 1061 GCATGT [GA]CATG^[CT] PaeR7I 52 CTCGAG C^TCGAG PflMI 1326 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1364 CTGCAG CTGCA^G Sau96I 1408 GGCCC G^G[ACGT]CC SfaNI 1210 GATGC GCATC(5/9) SspI 1118 AATATT AAT^ATT StuI 1431 AGGCCT AGG^CCT Van91I 1326 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 52 CTCGAG C^TCGAG XhoII 168 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 386 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 635 CAGAGGCTG AocI 829 CCTGAGG CC^T[ACGT]AGG 1186 CCTGAGG AspHI 1348 GTGCTC G[AT]GC[AT]^C 1375 GTGCAC BanI 624 GGTGCC G^G[CT][GA]CC 1191 GGTACC BfaI 691 CTAG C^TAG 1403 CTAG BmyI 1348 GTGCTC G[AGT]GC[ACT]^C 1375 GTGCAC BsiHKAI 1348 GTGCTC G[AT]GC[AT]^C 1375 GTGCAC BsmAI 481 GTCTC GTCTC(1/5) 1279 GTCTC Bsp1286I 1348 GTGCTC G[AGT]GC[ACT]^C 1375 GTGCAC BspMI 1194 ACCTGC ACCTGC(4/8) 699 GCAGGT Bsu36I 829 CCTGAGG CC^T[ACGT]AGG 1186 CCTGAGG Cac8I 522 GCTTGC GC[ACGT]^[ACGT]GC 843 GCAGGC Eco81I 829 CCTGAGG CC^T[ACGT]AGG 1186 CCTGAGG MaeI 691 CTAG C^TAG 1403 CTAG SmlI 40 CTCAAG C^T[CT][GA]AG 52 CTCGAG TaqI 53 TCGA T^CGA 926 TCGA TfiI 274 GAATC G^A[AT]TC 1465 GAATC TspRI 390 CACTG CA[GC]TG[ACGT][ACGT]^ 1373 CAGTG TthHB8I 53 TCGA T^CGA 926 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AfaI AluI BbvI BclI BseRI BsgI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI ScrFI SfcI Tru9I TseI Tsp45I Tsp509I