################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflIII 709 ACACGT A^C[GA][CT]GT AgeI 438 ACCGGT A^CCGGT AvaI 31 CCCGGG C^[CT]CG[GA]G BanII 28 GAGCCC G[GA]GC[CT]^C BbsI 367 GAAGAC GAAGAC(2/6) BcgI 402 GCAGGATACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 405 GGATAC GTATCC(6/5) BclI 1092 TGATCA T^GATCA BpuAI 367 GAAGAC GAAGAC(2/6) BsaAI 962 CACGTA [CT]AC^GT[GA] BsaI 1532 GAGACC GGTCTC(1/5) BsaWI 438 ACCGGT [AT]^CCGG[AT] BseRI 1358 GAGGAG GAGGAG(10/8) BsoBI 31 CCCGGG C^[CT]CG[GA]G BspDI 1264 ATCGAT AT^CGAT BspHI 1191 TCATGA T^CATGA BsrFI 438 ACCGGT [GA]^CCGG[CT] BssSI 410 CTCGTG CACGAG(-5/-1) BstYI 3 GGATCT [GA]^GATC[CT] Cfr10I 438 ACCGGT [GA]^CCGG[CT] ClaI 1264 ATCGAT AT^CGAT DraII 51 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 67 CCATGG C^C[GA][CT]GG EcoO109I 51 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 1028 GAATTC G^AATTC EcoRV 481 GATATC GAT^ATC EcoT14I 67 CCATGG C^C[AT][AT]GG EcoT22I 800 ATGCAT ATGCA^T FauI 653 CCCGC CCCGC(4/6) FbaI 1092 TGATCA T^GATCA HindIII 1457 AAGCTT A^AGCTT HphI 485 TCACC GGTGA(8/7) MflI 3 GGATCT [GA]^GATC[CT] MwoI 685 GCAGAAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 67 CCATGG C^CATGG NdeI 803 CATATG CA^TATG NsiI 800 ATGCAT ATGCA^T PinAI 438 ACCGGT A^CCGGT PpuMI 51 GGGACCC [GA]G^G[AT]CC[CT] PstI 1326 CTGCAG CTGCA^G PvuII 124 CAGCTG CAG^CTG RcaI 1191 TCATGA T^CATGA SmaI 31 CCCGGG CCC^GGG SphI 1242 GCATGC GCATG^C StyI 67 CCATGG C^C[AT][AT]GG TfiI 900 GAATC G^A[AT]TC XhoII 3 GGATCT [GA]^GATC[CT] XmaCI 31 CCCGGG C^CCGGG XmaI 31 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaII 52 GGACC G^G[AT]CC 190 GGACC BalI 128 TGGCCA TGG^CCA 1086 TGGCCA BmyI 28 GAGCCC G[AGT]GC[ACT]^C 650 GTGCCC BpmI 203 CTGGAG CTGGAG(16/14) 1306 CTGGAG BsmI 1013 GAATGC GAATGC(1/-1) 675 GCATTC Bsp1286I 28 GAGCCC G[AGT]GC[ACT]^C 650 GTGCCC BstNI 47 CCTGG CC^[AT]GG 1305 CCTGG Cfr13I 52 GGACC G^G[ACGT]CC 190 GGACC EaeI 128 TGGCCA [CT]^GGCC[GA] 1086 TGGCCA EarI 1166 CTCTTC CTCTTC(1/4) 1390 CTCTTC EcoRII 47 CCTGG ^CC[AT]GG 1305 CCTGG HincII 394 GTTGAC GT[CT]^[GA]AC 733 GTCAAC HindII 394 GTTGAC GT[CT]^[GA]AC 733 GTCAAC Ksp632I 1166 CTCTTC CTCTTC(1/4) 1390 CTCTTC MaeIII 227 GTGAC ^GT[ACGT]AC 905 GTGAC MluNI 128 TGGCCA TGG^CCA 1086 TGGCCA MscI 128 TGGCCA TGG^CCA 1086 TGGCCA MspA1I 41 CAGCGG C[AC]G^C[GT]G 124 CAGCTG MvaI 47 CCTGG CC^[AT]GG 1305 CCTGG PleI 784 GAGTC GAGTC(4/5) 1361 GAGTC Sau96I 52 GGACC G^G[ACGT]CC 190 GGACC Tsp45I 227 GTGAC ^GT[GC]AC 905 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BcnI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I Cac8I DdeI DpnII DraI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MboI MboII MnlI MseI MslI MspI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp509I TspRI TthHB8I