Restriction map for
pFN21AE3226
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflIII 709 ACACGT A^C[GA][CT]GT
AgeI 438 ACCGGT A^CCGGT
AvaI 31 CCCGGG C^[CT]CG[GA]G
BanII 28 GAGCCC G[GA]GC[CT]^C
BbsI 367 GAAGAC GAAGAC(2/6)
BcgI 402 GCAGGATACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 405 GGATAC GTATCC(6/5)
BclI 1092 TGATCA T^GATCA
BpuAI 367 GAAGAC GAAGAC(2/6)
BsaAI 962 CACGTA [CT]AC^GT[GA]
BsaI 1532 GAGACC GGTCTC(1/5)
BsaWI 438 ACCGGT [AT]^CCGG[AT]
BseRI 1358 GAGGAG GAGGAG(10/8)
BsoBI 31 CCCGGG C^[CT]CG[GA]G
BspDI 1264 ATCGAT AT^CGAT
BspHI 1191 TCATGA T^CATGA
BsrFI 438 ACCGGT [GA]^CCGG[CT]
BssSI 410 CTCGTG CACGAG(-5/-1)
BstYI 3 GGATCT [GA]^GATC[CT]
Cfr10I 438 ACCGGT [GA]^CCGG[CT]
ClaI 1264 ATCGAT AT^CGAT
DraII 51 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 67 CCATGG C^C[GA][CT]GG
EcoO109I 51 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1028 GAATTC G^AATTC
EcoRV 481 GATATC GAT^ATC
EcoT14I 67 CCATGG C^C[AT][AT]GG
EcoT22I 800 ATGCAT ATGCA^T
FauI 653 CCCGC CCCGC(4/6)
FbaI 1092 TGATCA T^GATCA
HindIII 1457 AAGCTT A^AGCTT
HphI 485 TCACC GGTGA(8/7)
MflI 3 GGATCT [GA]^GATC[CT]
MwoI 685 GCAGAAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 67 CCATGG C^CATGG
NdeI 803 CATATG CA^TATG
NsiI 800 ATGCAT ATGCA^T
PinAI 438 ACCGGT A^CCGGT
PpuMI 51 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1326 CTGCAG CTGCA^G
PvuII 124 CAGCTG CAG^CTG
RcaI 1191 TCATGA T^CATGA
SmaI 31 CCCGGG CCC^GGG
SphI 1242 GCATGC GCATG^C
StyI 67 CCATGG C^C[AT][AT]GG
TfiI 900 GAATC G^A[AT]TC
XhoII 3 GGATCT [GA]^GATC[CT]
XmaCI 31 CCCGGG C^CCGGG
XmaI 31 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaII 52 GGACC G^G[AT]CC
190 GGACC
BalI 128 TGGCCA TGG^CCA
1086 TGGCCA
BmyI 28 GAGCCC G[AGT]GC[ACT]^C
650 GTGCCC
BpmI 203 CTGGAG CTGGAG(16/14)
1306 CTGGAG
BsmI 1013 GAATGC GAATGC(1/-1)
675 GCATTC
Bsp1286I 28 GAGCCC G[AGT]GC[ACT]^C
650 GTGCCC
BstNI 47 CCTGG CC^[AT]GG
1305 CCTGG
Cfr13I 52 GGACC G^G[ACGT]CC
190 GGACC
EaeI 128 TGGCCA [CT]^GGCC[GA]
1086 TGGCCA
EarI 1166 CTCTTC CTCTTC(1/4)
1390 CTCTTC
EcoRII 47 CCTGG ^CC[AT]GG
1305 CCTGG
HincII 394 GTTGAC GT[CT]^[GA]AC
733 GTCAAC
HindII 394 GTTGAC GT[CT]^[GA]AC
733 GTCAAC
Ksp632I 1166 CTCTTC CTCTTC(1/4)
1390 CTCTTC
MaeIII 227 GTGAC ^GT[ACGT]AC
905 GTGAC
MluNI 128 TGGCCA TGG^CCA
1086 TGGCCA
MscI 128 TGGCCA TGG^CCA
1086 TGGCCA
MspA1I 41 CAGCGG C[AC]G^C[GT]G
124 CAGCTG
MvaI 47 CCTGG CC^[AT]GG
1305 CCTGG
PleI 784 GAGTC GAGTC(4/5)
1361 GAGTC
Sau96I 52 GGACC G^G[ACGT]CC
190 GGACC
Tsp45I 227 GTGAC ^GT[GC]AC
905 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BcnI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I Cac8I DdeI DpnII DraI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeII MboI MboII
MnlI MseI MslI MspI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI ScrFI SfaNI SfcI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I