Restriction map for
pFN21AE9507
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspA1I MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcsI 3 GAATTC [GA]^AATT[CT]
AcyI 322 GACGCC G[GA]^CG[CT]C
AflIII 620 ACATGT A^C[GA][CT]GT
AhdI 68 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 264 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 752 GGGCCC GGGCC^C
ApoI 3 GAATTC [GA]^AATT[CT]
AspEI 68 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 667 CCCGGG C^[CT]CG[GA]G
BalI 401 TGGCCA TGG^CCA
BanII 752 GGGCCC G[GA]GC[CT]^C
BfaI 310 CTAG C^TAG
BmyI 752 GGGCCC G[AGT]GC[ACT]^C
BsaHI 322 GACGCC G[GA]^CG[CT]C
BsaI 274 GAGACC GGTCTC(1/5)
BsmI 42 GAATGC GAATGC(1/-1)
BsoBI 667 CCCGGG C^[CT]CG[GA]G
Bsp1286I 752 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 611 TGTACA T^GTACA
BspHI 458 TCATGA T^CATGA
BspLU11I 620 ACATGT A^CATGT
BsrFI 490 GCCGGC [GA]^CCGG[CT]
BsrGI 611 TGTACA T^GTACA
BstAPI 509 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 711 GGTCACC G^GT[ACGT]ACC
BstPI 711 GGTCACC G^GT[ACGT]ACC
BstYI 30 GGATCT [GA]^GATC[CT]
Cfr10I 490 GCCGGC [GA]^CCGG[CT]
EaeI 401 TGGCCA [CT]^GGCC[GA]
Eam1105I 68 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 711 GGTCACC G^GT[ACGT]ACC
EcoRI 3 GAATTC G^AATTC
HaeII 470 GGCGCT [GA]GCGC^[CT]
HgaI 322 GACGC GACGC(5/10)
Hin1I 322 GACGCC G[GA]^CG[CT]C
MaeI 310 CTAG C^TAG
MflI 30 GGATCT [GA]^GATC[CT]
MluNI 401 TGGCCA TGG^CCA
MscI 401 TGGCCA TGG^CCA
NaeI 490 GCCGGC GCC^GGC
NgoMIV 490 GCCGGC G^CCGGC
PflMI 263 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 66 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 639 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 954 CTGCAG CTGCA^G
RcaI 458 TCATGA T^CATGA
SfcI 954 CTGCAG C^T[GA][CT]AG
SmaI 667 CCCGGG CCC^GGG
SphI 698 GCATGC GCATG^C
SspBI 611 TGTACA T^GTACA
TfiI 544 GAATC G^A[AT]TC
Van91I 263 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 30 GGATCT [GA]^GATC[CT]
XmaCI 667 CCCGGG C^CCGGG
XmaI 667 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BcnI 667 CCCGG CC^[GC]GG
668 CCGGG
BspMI 17 ACCTGC ACCTGC(4/8)
197 ACCTGC
BsrDI 111 CATTGC GCAATG(2/0)
405 CATTGC
BsrI 399 ACTGG ACTGG(1/-1)
263 CCAGT
BstXI 404 CCATTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
722 CCATGCTGCTGG
DraII 66 AGGACCC [GA]G^G[ACGT]CC[CT]
752 GGGCCCC
DraIII 92 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
307 CACCTAGTG
DsaI 374 CCGTGG C^C[GA][CT]GG
936 CCGTGG
EcoO109I 66 AGGACCC [GA]G^G[ACGT]CC[CT]
752 GGGCCCC
HapII 491 CCGG C^CGG
668 CCGG
HpaII 491 CCGG C^CGG
668 CCGG
MseI 778 TTAA T^TAA
917 TTAA
MspI 491 CCGG C^CGG
668 CCGG
NciI 667 CCCGG CC^[GC]GG
668 CCGGG
PleI 355 GACTC GAGTC(4/5)
639 GACTC
Tru9I 778 TTAA T^TAA
917 TTAA
Tsp509I 4 AATT ^AATT
859 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbsI BbvI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI