Restriction map for
pFN21AE3195
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AhdI AlwNI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MluI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 476 TCCGGA T^CCGGA
AfaI 524 GTAC GT^AC
AflIII 851 ACATGT A^C[GA][CT]GT
AgeI 1246 ACCGGT A^CCGGT
Alw44I 713 GTGCAC G^TGCAC
ApaI 39 GGGCCC GGGCC^C
ApaLI 713 GTGCAC G^TGCAC
AspI 739 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BpmI 536 CTCCAG CTGGAG(16/14)
BsaBI 414 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 476 TCCGGA T^CCGGA
BsgI 424 CTGCAC GTGCAG(16/14)
BsmAI 817 GAGAC GTCTC(1/5)
BspEI 476 TCCGGA T^CCGGA
BspLU11I 851 ACATGT A^CATGT
BsrDI 517 GCAATG GCAATG(2/0)
DsaI 1289 CCGTGG C^C[GA][CT]GG
EcoRI 625 GAATTC G^AATTC
EcoT14I 266 CCAAGG C^C[AT][AT]GG
HincII 754 GTTGAC GT[CT]^[GA]AC
HindII 754 GTTGAC GT[CT]^[GA]AC
MamI 414 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 506 CAATTG C^AATTG
MroI 476 TCCGGA T^CCGGA
MslI 427 CACGTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 506 CAATTG C^AATTG
PflFI 739 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1246 ACCGGT A^CCGGT
PvuII 700 CAGCTG CAG^CTG
RsaI 524 GTAC GT^AC
SacI 1366 GAGCTC GAGCT^C
ScaI 523 AGTACT AGT^ACT
SmlI 1354 CTCAAG C^T[CT][GA]AG
SspI 614 AATATT AAT^ATT
StyI 266 CCAAGG C^C[AT][AT]GG
Tth111I 739 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 464 AAATTC [GA]^AATT[CT]
625 GAATTC
ApoI 464 AAATTC [GA]^AATT[CT]
625 GAATTC
AspHI 713 GTGCAC G[AT]GC[AT]^C
1366 GAGCTC
BalI 548 TGGCCA TGG^CCA
867 TGGCCA
BamHI 419 GGATCC G^GATCC
893 GGATCC
BbsI 1117 GAAGAC GAAGAC(2/6)
1156 GAAGAC
BpuAI 1117 GAAGAC GAAGAC(2/6)
1156 GAAGAC
BsaWI 476 TCCGGA [AT]^CCGG[AT]
1246 ACCGGT
BsiHKAI 713 GTGCAC G[AT]GC[AT]^C
1366 GAGCTC
BspMI 344 ACCTGC ACCTGC(4/8)
1282 ACCTGC
EagI 22 CGGCCG C^GGCCG
128 CGGCCG
EclXI 22 CGGCCG C^GGCCG
128 CGGCCG
Eco52I 22 CGGCCG C^GGCCG
128 CGGCCG
FauI 94 CCCGC CCCGC(4/6)
1272 CCCGC
MaeII 428 ACGT A^CGT
929 ACGT
MluNI 548 TGGCCA TGG^CCA
867 TGGCCA
MscI 548 TGGCCA TGG^CCA
867 TGGCCA
NotI 21 GCGGCCGC GC^GGCCGC
127 GCGGCCGC
PleI 385 GAGTC GAGTC(4/5)
994 GAGTC
PpuMI 776 AGGACCT [GA]G^G[AT]CC[CT]
1217 GGGACCT
PstI 346 CTGCAG CTGCA^G
939 CTGCAG
SgrAI 74 CGCCGGTG C[GA]^CCGG[CT]G
1245 CACCGGTG
TspRI 603 CACTG CA[GC]TG[ACGT][ACGT]^
898 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiEI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TthHB8I
XhoII