Restriction map for
pFN21AE3084
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MflI MluI MroI
MseI MunI MvnI NaeI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcyI 316 GGCGCC G[GA]^CG[CT]C
AflIII 344 ACATGT A^C[GA][CT]GT
AlwNI 598 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 80 GTGCTC G[AT]GC[AT]^C
BalI 27 TGGCCA TGG^CCA
BbeI 316 GGCGCC GGCGC^C
BbsI 466 GTCTTC GAAGAC(2/6)
BclI 308 TGATCA T^GATCA
BfaI 553 CTAG C^TAG
BpuAI 466 GTCTTC GAAGAC(2/6)
BsaHI 316 GGCGCC G[GA]^CG[CT]C
BsiHKAI 80 GTGCTC G[AT]GC[AT]^C
BsmAI 127 GTCTC GTCTC(1/5)
BsmI 19 GCATTC GAATGC(1/-1)
Bsp1407I 341 TGTACA T^GTACA
BspLU11I 344 ACATGT A^CATGT
BspMI 7 ACCTGC ACCTGC(4/8)
BsrBI 584 GAGCGG CCGCTC(-3/-3)
BsrGI 341 TGTACA T^GTACA
BssSI 355 CTCGTG CACGAG(-5/-1)
BstF5I 471 CATCC GGATG(2/0)
BstXI 39 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 309 GATC ^GATC
DraIII 352 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 210 CCACGG C^C[GA][CT]GG
EaeI 27 TGGCCA [CT]^GGCC[GA]
EcoNI 500 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 586 GCGGG CCCGC(4/6)
FbaI 308 TGATCA T^GATCA
FokI 471 CATCC GGATG(9/13)
Hin1I 316 GGCGCC G[GA]^CG[CT]C
HincII 190 GTCAAC GT[CT]^[GA]AC
HindII 190 GTCAAC GT[CT]^[GA]AC
KasI 316 GGCGCC G^GCGCC
MaeI 553 CTAG C^TAG
MaeII 32 ACGT A^CGT
MboI 309 GATC ^GATC
MluNI 27 TGGCCA TGG^CCA
MscI 27 TGGCCA TGG^CCA
MslI 150 CACCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 316 GGCGCC GG^CGCC
NdeI 728 CATATG CA^TATG
NdeII 309 GATC ^GATC
NspI 344 ACATGT [GA]CATG^[CT]
PpuMI 49 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 301 CAGCTG CAG^CTG
Sau3AI 309 GATC ^GATC
SfoI 316 GGCGCC GGC^GCC
SmlI 75 CTTGAG C^T[CT][GA]AG
SspBI 341 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 342 GTAC GT^AC
504 GTAC
Asp700I 537 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
540 GAAGTTCTTC
AvaI 260 CCCGGG C^[CT]CG[GA]G
320 CCCGGG
AvaII 50 GGACC G^G[AT]CC
629 GGACC
BsgI 111 CTGCAC GTGCAG(16/14)
404 GTGCAG
BsoBI 260 CCCGGG C^[CT]CG[GA]G
320 CCCGGG
CfoI 224 GCGC GCG^C
317 GCGC
EcoT14I 45 CCTTGG C^C[AT][AT]GG
652 CCAAGG
HaeII 223 AGCGCC [GA]GCGC^[CT]
316 GGCGCC
HhaI 224 GCGC GCG^C
317 GCGC
HinP1I 224 GCGC G^CGC
317 GCGC
HinfI 325 GACTC G^A[ACGT]TC
637 GACTC
HphI 306 GGTGA GGTGA(8/7)
682 TCACC
MaeIII 385 GTCAC ^GT[ACGT]AC
457 GTCAC
PleI 325 GACTC GAGTC(4/5)
637 GACTC
RsaI 342 GTAC GT^AC
504 GTAC
SmaI 260 CCCGGG CCC^GGG
320 CCCGGG
StyI 45 CCTTGG C^C[AT][AT]GG
652 CCAAGG
Tsp45I 385 GTCAC ^GT[GC]AC
457 GTCAC
XmaCI 260 CCCGGG C^CCGGG
320 CCCGGG
XmaI 260 CCCGGG C^CCGGG
320 CCCGGG
XmnI 537 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
540 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI TspRI