Restriction map for
pFN21AE6654
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccIII AclI AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MboII
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 549 GACGTC GACGT^C
AccI 442 GTCTAC GT^[AC][GT]AC
AcyI 549 GACGTC G[GA]^CG[CT]C
AlwNI 73 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 353 TGGCCA TGG^CCA
BanI 460 GGTGCC G^G[CT][GA]CC
BbrPI 640 CACGTG CAC^GTG
BbvCI 628 GCTGAGG CCTCAGC(-5/-2)
BfaI 493 CTAG C^TAG
BsaAI 640 CACGTG [CT]AC^GT[GA]
BsaHI 549 GACGTC G[GA]^CG[CT]C
BsgI 79 CTGCAC GTGCAG(16/14)
BsmAI 400 GTCTC GTCTC(1/5)
BsmFI 379 GTCCC GGGAC(10/14)
BsmI 522 GAATGC GAATGC(1/-1)
BsrBI 202 GAGCGG CCGCTC(-3/-3)
BsrFI 705 GCCGGT [GA]^CCGG[CT]
BssHII 92 GCGCGC G^CGCGC
BstXI 479 CCAAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 705 GCCGGT [GA]^CCGG[CT]
DraIII 640 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 133 GACAGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 353 TGGCCA [CT]^GGCC[GA]
HgaI 440 GCGTC GACGC(5/10)
Hin1I 549 GACGTC G[GA]^CG[CT]C
MaeI 493 CTAG C^TAG
MluNI 353 TGGCCA TGG^CCA
MscI 353 TGGCCA TGG^CCA
MseI 364 TTAA T^TAA
NcoI 554 CCATGG C^CATGG
NspI 470 ACATGC [GA]CATG^[CT]
PmaCI 640 CACGTG CAC^GTG
PmlI 640 CACGTG CAC^GTG
PstI 265 CTGCAG CTGCA^G
SacI 496 GAGCTC GAGCT^C
SfcI 265 CTGCAG C^T[GA][CT]AG
StuI 293 AGGCCT AGG^CCT
TfiI 608 GAATC G^A[AT]TC
Tru9I 364 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 5 AGCT AG^CT
497 AGCT
ApaI 222 GGGCCC GGGCC^C
502 GGGCCC
BspMI 233 ACCTGC ACCTGC(4/8)
320 ACCTGC
BsrI 188 ACTGG ACTGG(1/-1)
408 CCAGT
BstEII 33 GGTCACC G^GT[ACGT]ACC
282 GGTCACC
BstF5I 333 CATCC GGATG(2/0)
654 CATCC
BstPI 33 GGTCACC G^GT[ACGT]ACC
282 GGTCACC
DraII 222 GGGCCCC [GA]G^G[ACGT]CC[CT]
502 GGGCCCC
EcoO109I 222 GGGCCCC [GA]G^G[ACGT]CC[CT]
502 GGGCCCC
EcoO65I 33 GGTCACC G^GT[ACGT]ACC
282 GGTCACC
FokI 333 CATCC GGATG(9/13)
654 CATCC
HaeII 75 GGCGCT [GA]GCGC^[CT]
207 GGCGCT
MaeII 550 ACGT A^CGT
641 ACGT
MslI 333 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
537 CACAGTGGTG
MspA1I 251 CCGCTG C[AC]G^C[GT]G
418 CAGCGG
PleI 117 GACTC GAGTC(4/5)
377 GAGTC
SmaI 153 CCCGGG CCC^GGG
371 CCCGGG
TspRI 409 CAGTG CA[GC]TG[ACGT][ACGT]^
539 CAGTG
XcmI 455 CCAAGGGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
698 CCACTTTGCCGGTGG
XmaCI 153 CCCGGG C^CCGGG
371 CCCGGG
XmaI 153 CCCGGG C^CCGGG
371 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AlwI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MflI MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
TseI Tsp45I XhoII