Restriction map for
pFN21AE5546
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI AocI
Aor51HI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsiEI BsmBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrGI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MscI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 5 TCCGGA T^CCGGA
AflIII 839 ACGTGT A^C[GA][CT]GT
AscI 59 GGCGCGCC GG^CGCGCC
AvaI 48 CCCGGG C^[CT]CG[GA]G
BciVI 568 GTATCC GTATCC(6/5)
BfaI 295 CTAG C^TAG
BglI 47 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 838 TACGTG [CT]AC^GT[GA]
BsaI 298 GAGACC GGTCTC(1/5)
BsaWI 5 TCCGGA [AT]^CCGG[AT]
BseAI 5 TCCGGA T^CCGGA
BsgI 238 GTGCAG GTGCAG(16/14)
BsiWI 866 CGTACG C^GTACG
BsmFI 368 GGGAC GGGAC(10/14)
BsmI 409 GAATGC GAATGC(1/-1)
BsoBI 48 CCCGGG C^[CT]CG[GA]G
BspEI 5 TCCGGA T^CCGGA
BspHI 794 TCATGA T^CATGA
BsrDI 338 GCAATG GCAATG(2/0)
BsrFI 833 GCCGGT [GA]^CCGG[CT]
BssHII 60 GCGCGC G^CGCGC
BssSI 445 CTCGTG CACGAG(-5/-1)
BstEII 465 GGTAACC G^GT[ACGT]ACC
BstPI 465 GGTAACC G^GT[ACGT]ACC
Cfr10I 833 GCCGGT [GA]^CCGG[CT]
DsaI 140 CCATGG C^C[GA][CT]GG
EcoNI 696 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 465 GGTAACC G^GT[ACGT]ACC
MaeI 295 CTAG C^TAG
MroI 5 TCCGGA T^CCGGA
NcoI 140 CCATGG C^CATGG
PflMI 245 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 813 GACTC GAGTC(4/5)
PvuII 121 CAGCTG CAG^CTG
RcaI 794 TCATGA T^CATGA
SacI 883 GAGCTC GAGCT^C
SexAI 908 ACCTGGT A^CC[AT]GGT
SfaNI 986 GCATC GCATC(5/9)
SmaI 48 CCCGGG CCC^GGG
SphI 455 GCATGC GCATG^C
TaqI 665 TCGA T^CGA
TfiI 798 GAATC G^A[AT]TC
Tsp509I 933 AATT ^AATT
TspRI 1005 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 665 TCGA T^CGA
Van91I 245 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 48 CCCGGG C^CCGGG
XmaI 48 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 166 GTGCAC G^TGCAC
706 GTGCAC
AlwI 3 GATCC GGATC(4/5)
1043 GGATC
AlwNI 574 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1138 CAGCCCCTG
ApaI 45 GGGCCC GGGCC^C
199 GGGCCC
ApaLI 166 GTGCAC G^TGCAC
706 GTGCAC
AvaII 560 GGACC G^G[AT]CC
906 GGACC
BanI 267 GGTGCC G^G[CT][GA]CC
658 GGTGCC
BpmI 697 CTGGAG CTGGAG(16/14)
1111 CTGGAG
BseRI 178 GAGGAG GAGGAG(10/8)
220 GAGGAG
BsmAI 298 GAGAC GTCTC(1/5)
637 GAGAC
BsrI 573 CCAGT ACTGG(1/-1)
603 CCAGT
BstF5I 600 CATCC GGATG(2/0)
948 GGATG
BstXI 1052 CCAACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1094 CCAAGGAGCTGG
DrdI 754 GACCAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1027 GACCTCATCGTC
EaeI 108 CGGCCA [CT]^GGCC[GA]
363 TGGCCG
EcoT14I 140 CCATGG C^C[AT][AT]GG
1094 CCAAGG
FauI 17 GCGGG CCCGC(4/6)
739 GCGGG
FokI 600 CATCC GGATG(9/13)
948 GGATG
HinfI 798 GAATC G^A[ACGT]TC
813 GACTC
MaeII 284 ACGT A^CGT
839 ACGT
NspI 455 GCATGC [GA]CATG^[CT]
684 GCATGT
PstI 23 CTGCAG CTGCA^G
580 CTGCAG
StyI 140 CCATGG C^C[AT][AT]GG
1094 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI TseI Tsp45I XcmI