Restriction map for
pFN21AB6868
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BseRI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AluI 210 AGCT AG^CT
AlwNI 399 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 451 GTGCTC G[AT]GC[AT]^C
AvaII 269 GGTCC G^G[AT]CC
BamHI 238 GGATCC G^GATCC
BbsI 261 GTCTTC GAAGAC(2/6)
BclI 297 TGATCA T^GATCA
BcnI 419 CCCGG CC^[GC]GG
BpmI 575 CTGGAG CTGGAG(16/14)
BpuAI 261 GTCTTC GAAGAC(2/6)
BsaI 304 GAGACC GGTCTC(1/5)
BsaWI 235 ACCGGA [AT]^CCGG[AT]
BsiHKAI 451 GTGCTC G[AT]GC[AT]^C
BsmBI 329 CGTCTC CGTCTC(1/5)
BsmFI 270 GTCCC GGGAC(10/14)
BspMI 502 GCAGGT ACCTGC(4/8)
BsrDI 473 CATTGC GCAATG(2/0)
BstAPI 156 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 238 GGATCC [GA]^GATC[CT]
DdeI 319 CTCAG C^T[ACGT]AG
DsaI 117 CCATGG C^C[GA][CT]GG
EarI 256 GAAGAG CTCTTC(1/4)
EcoT22I 470 ATGCAT ATGCA^T
FbaI 297 TGATCA T^GATCA
HinfI 259 GAGTC G^A[ACGT]TC
HphI 6 GGTGA GGTGA(8/7)
Ksp632I 256 GAAGAG CTCTTC(1/4)
MaeII 328 ACGT A^CGT
MaeIII 356 GTGAC ^GT[ACGT]AC
MflI 238 GGATCC [GA]^GATC[CT]
MslI 349 CATTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 434 CCGCTG C[AC]G^C[GT]G
NciI 419 CCCGG CC^[GC]GG
NcoI 117 CCATGG C^CATGG
NsiI 470 ATGCAT ATGCA^T
NspI 468 ACATGC [GA]CATG^[CT]
PflMI 217 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 259 GAGTC GAGTC(4/5)
PpuMI 268 AGGTCCC [GA]G^G[AT]CC[CT]
SmlI 482 CTCAAG C^T[CT][GA]AG
Tsp45I 356 GTGAC ^GT[GC]AC
Van91I 217 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 238 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 239 GATCC GGATC(4/5)
238 GGATC
ApaI 182 GGGCCC GGGCC^C
394 GGGCCC
BalI 32 TGGCCA TGG^CCA
50 TGGCCA
BanII 182 GGGCCC G[GA]GC[CT]^C
394 GGGCCC
BsgI 491 CTGCAC GTGCAG(16/14)
506 GTGCAG
BsmAI 304 GAGAC GTCTC(1/5)
330 GTCTC
BsrI 308 CCAGT ACTGG(1/-1)
448 CCAGT
EaeI 32 TGGCCA [CT]^GGCC[GA]
50 TGGCCA
EcoT14I 117 CCATGG C^C[AT][AT]GG
334 CCTTGG
FauI 153 CCCGC CCCGC(4/6)
433 CCCGC
HapII 236 CCGG C^CGG
420 CCGG
HpaII 236 CCGG C^CGG
420 CCGG
MluNI 32 TGGCCA TGG^CCA
50 TGGCCA
MscI 32 TGGCCA TGG^CCA
50 TGGCCA
MseI 14 TTAA T^TAA
89 TTAA
MspI 236 CCGG C^CGG
420 CCGG
PstI 75 CTGCAG CTGCA^G
386 CTGCAG
StyI 117 CCATGG C^C[AT][AT]GG
334 CCTTGG
Tru9I 14 TTAA T^TAA
89 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI BanI BbvI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII ItaI MaeI MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI