Restriction map for
pFN21AE5269
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BciVI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NarI NciI
NcoI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 264 AACGTT AA^CGTT
AfaI 177 GTAC GT^AC
Alw44I 149 GTGCAC G^TGCAC
AlwI 273 GATCC GGATC(4/5)
AlwNI 193 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 149 GTGCAC G^TGCAC
AvaII 201 GGTCC G^G[AT]CC
BanII 250 GAGCTC G[GA]GC[CT]^C
BbvCI 535 GCTGAGG CCTCAGC(-5/-2)
BcgI 464 GCATCAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 434 TGATCA T^GATCA
BseRI 111 GAGGAG GAGGAG(10/8)
BsiEI 11 CGGCCG CG[GA][CT]^CG
BsrBI 14 CCGCTC CCGCTC(-3/-3)
BsrFI 6 GCCGGC [GA]^CCGG[CT]
BstYI 272 AGATCC [GA]^GATC[CT]
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
EcoRV 358 GATATC GAT^ATC
FbaI 434 TGATCA T^GATCA
HapII 7 CCGG C^CGG
HinfI 496 GAGTC G^A[ACGT]TC
HpaII 7 CCGG C^CGG
HphI 389 TCACC GGTGA(8/7)
MaeII 265 ACGT A^CGT
MflI 272 AGATCC [GA]^GATC[CT]
MspI 7 CCGG C^CGG
NaeI 6 GCCGGC GCC^GGC
NdeI 106 CATATG CA^TATG
NgoMIV 6 GCCGGC G^CCGGC
NlaIII 86 CATG CATG^
NotI 10 GCGGCCGC GC^GGCCGC
PleI 496 GAGTC GAGTC(4/5)
Psp1406I 264 AACGTT AA^CGTT
PstI 329 CTGCAG CTGCA^G
PvuII 196 CAGCTG CAG^CTG
RsaI 177 GTAC GT^AC
SacI 250 GAGCTC GAGCT^C
SfaNI 464 GCATC GCATC(5/9)
SfcI 329 CTGCAG C^T[GA][CT]AG
XhoII 272 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AspHI 149 GTGCAC G[AT]GC[AT]^C
250 GAGCTC
BanI 81 GGTGCC G^G[CT][GA]CC
577 GGCACC
BglI 337 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
559 GCCCTGGAGGC
BmyI 149 GTGCAC G[AGT]GC[ACT]^C
250 GAGCTC
BpmI 562 CTGGAG CTGGAG(16/14)
589 CTGGAG
BsiHKAI 149 GTGCAC G[AT]GC[AT]^C
250 GAGCTC
BsmFI 322 GTCCC GGGAC(10/14)
498 GTCCC
Bsp1286I 149 GTGCAC G[AGT]GC[ACT]^C
250 GAGCTC
BstF5I 456 CATCC GGATG(2/0)
279 GGATG
DdeI 237 CTCAG C^T[ACGT]AG
536 CTGAG
DraII 30 GGGGCCT [GA]G^G[ACGT]CC[CT]
137 AGGCCCT
DsaI 416 CCGTGG C^C[GA][CT]GG
581 CCGTGG
EaeI 11 CGGCCG [CT]^GGCC[GA]
120 CGGCCA
EcoO109I 30 GGGGCCT [GA]G^G[ACGT]CC[CT]
137 AGGCCCT
FokI 456 CATCC GGATG(9/13)
279 GGATG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
DpnII EcoNI EcoRII EcoT14I Fnu4HI
HaeIII ItaI MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI StyI TseI