Restriction map for
pFN21AE4221
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsiEI BsiWI
BsmI BspDI BspHI BspLU11I BsrDI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI EclXI Eco52I EcoNI
EcoRI EcoRV FbaI FseI FspI
HindIII HpaI KpnI MaeI MamI
MfeI MluI MseI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SbfI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspI SwaI TfiI
Tru9I Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1963 GACGTC GACGT^C
AccI 561 GTCGAC GT^[AC][GT]AC
AflIII 1040 ACGTGT A^C[GA][CT]GT
AocI 2244 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1869 AGCGCT AGC^GCT
AspI 1471 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1611 GGCGCC GGCGC^C
BbrPI 1759 CACGTG CAC^GTG
BbsI 1513 GAAGAC GAAGAC(2/6)
BpmI 874 CTGGAG CTGGAG(16/14)
BpuAI 1513 GAAGAC GAAGAC(2/6)
BsaAI 1759 CACGTG [CT]AC^GT[GA]
BsaI 1782 GGTCTC GGTCTC(1/5)
BsrBI 1348 GAGCGG CCGCTC(-3/-3)
BssSI 1330 CACGAG CACGAG(-5/-1)
BstEII 181 GGTTACC G^GT[ACGT]ACC
BstPI 181 GGTTACC G^GT[ACGT]ACC
Bsu36I 2244 CCTGAGG CC^T[ACGT]AGG
DrdI 1084 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1813 CTCTTC CTCTTC(1/4)
Eco47III 1869 AGCGCT AGC^GCT
Eco81I 2244 CCTGAGG CC^T[ACGT]AGG
EcoO65I 181 GGTTACC G^GT[ACGT]ACC
EcoT14I 1590 CCAAGG C^C[AT][AT]GG
EcoT22I 1110 ATGCAT ATGCA^T
HincII 561 GTCGAC GT[CT]^[GA]AC
HindII 561 GTCGAC GT[CT]^[GA]AC
KasI 1611 GGCGCC G^GCGCC
Ksp632I 1813 CTCTTC CTCTTC(1/4)
NarI 1611 GGCGCC GG^CGCC
NsiI 1110 ATGCAT ATGCA^T
PflFI 1471 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1773 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1759 CACGTG CAC^GTG
PmlI 1759 CACGTG CAC^GTG
SalI 561 GTCGAC G^TCGAC
SapI 1812 GCTCTTC GCTCTTC(1/4)
ScaI 1382 AGTACT AGT^ACT
SfoI 1611 GGCGCC GGC^GCC
SmaI 1700 CCCGGG CCC^GGG
StyI 1590 CCAAGG C^C[AT][AT]GG
Tth111I 1471 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1773 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1700 CCCGGG C^CCGGG
XmaI 1700 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccIII 1478 TCCGGA T^CCGGA
2048 TCCGGA
AhdI 564 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1027 GACAAGCTGTC
Alw44I 136 GTGCAC G^TGCAC
2172 GTGCAC
ApaI 1895 GGGCCC GGGCC^C
2262 GGGCCC
ApaLI 136 GTGCAC G^TGCAC
2172 GTGCAC
AspEI 564 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1027 GACAAGCTGTC
BamHI 1544 GGATCC G^GATCC
2093 GGATCC
BcgI 380 CGACGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2057 CGAGGAGTTTGC
BsaWI 1478 TCCGGA [AT]^CCGG[AT]
2048 TCCGGA
BseAI 1478 TCCGGA T^CCGGA
2048 TCCGGA
BsmBI 1191 CGTCTC CGTCTC(1/5)
1153 GAGACG
BspEI 1478 TCCGGA T^CCGGA
2048 TCCGGA
BspMI 1733 ACCTGC ACCTGC(4/8)
1089 GCAGGT
Eam1105I 564 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1027 GACAAGCTGTC
MroI 1478 TCCGGA T^CCGGA
2048 TCCGGA
NaeI 338 GCCGGC GCC^GGC
799 GCCGGC
NgoMIV 338 GCCGGC G^CCGGC
799 GCCGGC
PvuII 964 CAGCTG CAG^CTG
1978 CAGCTG
SexAI 1604 ACCTGGT A^CC[AT]GGT
1778 ACCTGGT
SfiI 1887 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1938 GGCCATCGTGGCC
SphI 452 GCATGC GCATG^C
1112 GCATGC
StuI 947 AGGCCT AGG^CCT
1886 AGGCCT
TaqI 562 TCGA T^CGA
1970 TCGA
Tsp45I 583 GTCAC ^GT[GC]AC
770 GTGAC
TspRI 1051 CAGTG CA[GC]TG[ACGT][ACGT]^
1458 CACTG
TthHB8I 562 TCGA T^CGA
1970 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI TseI
XcmI XhoII