Restriction map for
pFN21AE6274
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BanI BbeI
BbsI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MfeI MluI
MroI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 412 GAATTT [GA]^AATT[CT]
AgeI 360 ACCGGT A^CCGGT
AhdI 298 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 412 GAATTT [GA]^AATT[CT]
AspEI 298 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 313 GTGCTC G[AT]GC[AT]^C
AvaII 522 GGTCC G^G[AT]CC
BalI 128 TGGCCA TGG^CCA
BamHI 201 GGATCC G^GATCC
BbrPI 481 CACGTG CAC^GTG
BcgI 408 CGAGGAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 95 CCCGG CC^[GC]GG
BsaAI 481 CACGTG [CT]AC^GT[GA]
BsaBI 21 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 360 ACCGGT [AT]^CCGG[AT]
BseRI 49 GAGGAG GAGGAG(10/8)
BsiHKAI 313 GTGCTC G[AT]GC[AT]^C
BsmAI 436 GAGAC GTCTC(1/5)
BsmBI 436 GAGACG CGTCTC(1/5)
BsmFI 523 GTCCC GGGAC(10/14)
BstF5I 132 CATCC GGATG(2/0)
CfoI 33 GCGC GCG^C
DdeI 434 CTGAG C^T[ACGT]AG
DraIII 349 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 298 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 558 CTCTTC CTCTTC(1/4)
FokI 132 CATCC GGATG(9/13)
HgaI 438 GACGC GACGC(5/10)
HhaI 33 GCGC GCG^C
HinP1I 33 GCGC G^CGC
HinfI 251 GAGTC G^A[ACGT]TC
HphI 138 GGTGA GGTGA(8/7)
Ksp632I 558 CTCTTC CTCTTC(1/4)
MamI 21 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 559 TCTTC GAAGA(8/7)
MluNI 128 TGGCCA TGG^CCA
MscI 128 TGGCCA TGG^CCA
NciI 95 CCCGG CC^[GC]GG
PinAI 360 ACCGGT A^CCGGT
PleI 251 GAGTC GAGTC(4/5)
PmaCI 481 CACGTG CAC^GTG
PmlI 481 CACGTG CAC^GTG
PpuMI 521 AGGTCCC [GA]G^G[AT]CC[CT]
ScaI 173 AGTACT AGT^ACT
SfcI 474 CTACAG C^T[GA][CT]AG
SmlI 343 CTCAAG C^T[CT][GA]AG
TaqI 29 TCGA T^CGA
Tsp509I 413 AATT ^AATT
TthHB8I 29 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 174 GTAC GT^AC
366 GTAC
ApaI 92 GGGCCC GGGCC^C
260 GGGCCC
BpmI 208 CTGGAG CTGGAG(16/14)
394 CTGGAG
BsgI 79 GTGCAG GTGCAG(16/14)
514 GTGCAG
BspMI 284 ACCTGC ACCTGC(4/8)
519 GCAGGT
BsrBI 40 GAGCGG CCGCTC(-3/-3)
217 GAGCGG
BsrFI 360 ACCGGT [GA]^CCGG[CT]
543 ACCGGC
BsrI 183 CCAGT ACTGG(1/-1)
419 CCAGT
BstYI 201 GGATCC [GA]^GATC[CT]
374 AGATCC
Cfr10I 360 ACCGGT [GA]^CCGG[CT]
543 ACCGGC
MaeII 119 ACGT A^CGT
482 ACGT
MaeIII 422 GTGAC ^GT[ACGT]AC
484 GTGAC
MflI 201 GGATCC [GA]^GATC[CT]
374 AGATCC
MslI 132 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
270 CACTGTCGTG
PvuII 108 CAGCTG CAG^CTG
229 CAGCTG
RsaI 174 GTAC GT^AC
366 GTAC
Tsp45I 422 GTGAC ^GT[GC]AC
484 GTGAC
XhoII 201 GGATCC [GA]^GATC[CT]
374 AGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I Cfr13I DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII HapII HpaII ItaI MboI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI TspRI