Restriction map for
pFN21AE6191
##################################
# NO CUT ENZYMES ( 194 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BcnI BfrI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MaeIII MamI
MflI MluI MroI MslI MspA1I
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I TspRI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 477 GAATTT [GA]^AATT[CT]
ApoI 477 GAATTT [GA]^AATT[CT]
BalI 182 TGGCCA TGG^CCA
BbvI 127 GCTGC GCAGC(8/12)
BciVI 447 GTATCC GTATCC(6/5)
BclI 384 TGATCA T^GATCA
BfaI 83 CTAG C^TAG
BglI 175 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 117 CTCCAG CTGGAG(16/14)
BsaI 106 GAGACC GGTCTC(1/5)
BsiYI 29 CCATTGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 29 CCATTGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 78 CATTGC GCAATG(2/0)
Cac8I 190 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 226 GCGC GCG^C
EaeI 182 TGGCCA [CT]^GGCC[GA]
EcoT14I 185 CCAAGG C^C[AT][AT]GG
FbaI 384 TGATCA T^GATCA
Fnu4HI 127 GCTGC GC^[ACGT]GC
HhaI 226 GCGC GCG^C
HinP1I 226 GCGC G^CGC
HindIII 413 AAGCTT A^AGCTT
HinfI 141 GAATC G^A[ACGT]TC
ItaI 127 GCTGC GC^[ACGT]GC
MaeI 83 CTAG C^TAG
MfeI 470 CAATTG C^AATTG
MluNI 182 TGGCCA TGG^CCA
MscI 182 TGGCCA TGG^CCA
MunI 470 CAATTG C^AATTG
MwoI 175 GCCAATGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 234 GGTTCC GG[ACGT]^[ACGT]CC
PflMI 29 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 271 GATGC GCATC(5/9)
SfiI 174 GGCCAATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 185 CCAAGG C^C[AT][AT]GG
TaqI 155 TCGA T^CGA
TfiI 141 GAATC G^A[AT]TC
TseI 127 GCTGC G^C[AT]GC
TthHB8I 155 TCGA T^CGA
Van91I 29 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BsaJI 185 CCAAGG C^C[ACGT][ACGT]GG
263 CCCAGG
BsmAI 106 GAGAC GTCTC(1/5)
315 GTCTC
BsrI 35 ACTGG ACTGG(1/-1)
158 ACTGG
BstF5I 201 GGATG GGATG(2/0)
270 GGATG
DdeI 137 CTGAG C^T[ACGT]AG
305 CTGAG
DpnII 54 GATC ^GATC
385 GATC
FokI 201 GGATG GGATG(9/13)
270 GGATG
HphI 113 TCACC GGTGA(8/7)
462 TCACC
MboI 54 GATC ^GATC
385 GATC
MseI 221 TTAA T^TAA
373 TTAA
NdeII 54 GATC ^GATC
385 GATC
Sau3AI 54 GATC ^GATC
385 GATC
Tru9I 221 TTAA T^TAA
373 TTAA
XcmI 148 CCAGCTATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
185 CCAAGGCTGGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI BssKI BstNI EcoRII HaeIII
MboII MnlI MvaI ScrFI Tsp509I