Restriction map for
pFN21AE3144
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbrPI BbsI BcgI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT14I FseI FspI HgaI
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MscI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 299 GTCTAC GT^[AC][GT]AC
AccIII 305 TCCGGA T^CCGGA
AcyI 37 GGCGCC G[GA]^CG[CT]C
AhdI 925 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 262 AGCT AG^CT
AlwI 308 GGATC GGATC(4/5)
AocI 1053 CCTGAGG CC^T[ACGT]AGG
AspEI 925 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 625 GTGCTC G[AT]GC[AT]^C
BanI 37 GGCGCC G^G[CT][GA]CC
BbeI 37 GGCGCC GGCGC^C
BbvCI 209 CCTCAGC CCTCAGC(-5/-2)
BciVI 728 GTATCC GTATCC(6/5)
BclI 401 TGATCA T^GATCA
BfaI 574 CTAG C^TAG
BsaHI 37 GGCGCC G[GA]^CG[CT]C
BsaI 739 GAGACC GGTCTC(1/5)
BsaWI 305 TCCGGA [AT]^CCGG[AT]
BseAI 305 TCCGGA T^CCGGA
BsgI 868 CTGCAC GTGCAG(16/14)
BsiHKAI 625 GTGCTC G[AT]GC[AT]^C
BsmI 993 GAATGC GAATGC(1/-1)
BspEI 305 TCCGGA T^CCGGA
BspMI 591 ACCTGC ACCTGC(4/8)
BsrBI 177 GAGCGG CCGCTC(-3/-3)
BsrDI 548 GCAATG GCAATG(2/0)
BssSI 736 CACGAG CACGAG(-5/-1)
Bsu36I 1053 CCTGAGG CC^T[ACGT]AGG
DrdI 953 GACTGATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 222 CCACGG C^C[GA][CT]GG
Eam1105I 925 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1053 CCTGAGG CC^T[ACGT]AGG
EcoRV 319 GATATC GAT^ATC
EcoT22I 395 ATGCAT ATGCA^T
FbaI 401 TGATCA T^GATCA
HaeII 37 GGCGCC [GA]GCGC^[CT]
Hin1I 37 GGCGCC G[GA]^CG[CT]C
HincII 376 GTTGAC GT[CT]^[GA]AC
HindII 376 GTTGAC GT[CT]^[GA]AC
HphI 807 GGTGA GGTGA(8/7)
KasI 37 GGCGCC G^GCGCC
MaeI 574 CTAG C^TAG
MaeII 960 ACGT A^CGT
MaeIII 761 GTGAC ^GT[ACGT]AC
MboII 440 TCTTC GAAGA(8/7)
MroI 305 TCCGGA T^CCGGA
MseI 530 TTAA T^TAA
NarI 37 GGCGCC GG^CGCC
NsiI 395 ATGCAT ATGCA^T
SfoI 37 GGCGCC GGC^GCC
SmlI 564 CTTGAG C^T[CT][GA]AG
TfiI 390 GATTC G^A[AT]TC
Tru9I 530 TTAA T^TAA
Tsp45I 761 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccII 231 CGCG CG^CG
288 CGCG
AfaI 364 GTAC GT^AC
578 GTAC
AlwNI 212 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
640 CAGTCCCTG
ApaI 51 GGGCCC GGGCC^C
168 GGGCCC
BglI 40 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
220 GCCCACGGGGC
BlpI 237 GCTTAGC GC^T[ACGT]AGC
258 GCTCAGC
Bpu1102I 237 GCTTAGC GC^T[ACGT]AGC
258 GCTCAGC
BstUI 231 CGCG CG^CG
288 CGCG
CelII 237 GCTTAGC GC^T[ACGT]AGC
258 GCTCAGC
EcoNI 343 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
424 CCTTATGAAGG
MvnI 231 CGCG CG^CG
288 CGCG
PleI 1006 GACTC GAGTC(4/5)
9 GAGTC
PpuMI 248 GGGACCC [GA]G^G[AT]CC[CT]
893 AGGACCC
RsaI 364 GTAC GT^AC
578 GTAC
SfaNI 942 GATGC GCATC(5/9)
614 GCATC
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SmaI TseI TspRI XmaCI
XmaI