Restriction map for
pFN21AB8893
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BbvI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsgI
BsiHKAI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KpnI MaeI MaeII MamI MfeI
MluI MluNI MscI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I TseI Tsp45I
Tth111I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 217 GTCTAC GT^[AC][GT]AC
AccIII 371 TCCGGA T^CCGGA
AcsI 463 GAATTT [GA]^AATT[CT]
AfaI 174 GTAC GT^AC
AlwI 374 GGATC GGATC(4/5)
ApaI 597 GGGCCC GGGCC^C
ApoI 463 GAATTT [GA]^AATT[CT]
AvaII 605 GGACC G^G[AT]CC
BanI 325 GGCGCC G^G[CT][GA]CC
BbeI 325 GGCGCC GGCGC^C
BcgI 500 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 313 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 371 TCCGGA T^CCGGA
BseRI 634 GAGGAG GAGGAG(10/8)
BsmI 21 GAATGC GAATGC(1/-1)
BspEI 371 TCCGGA T^CCGGA
BsrBI 131 GAGCGG CCGCTC(-3/-3)
BssHII 266 GCGCGC G^CGCGC
BstF5I 386 GGATG GGATG(2/0)
BstYI 374 GGATCT [GA]^GATC[CT]
DdeI 396 CTCAG C^T[ACGT]AG
DsaI 593 CCGCGG C^C[GA][CT]GG
FokI 386 GGATG GGATG(9/13)
KasI 325 GGCGCC G^GCGCC
KspI 593 CCGCGG CCGC^GG
MaeIII 365 GTAAC ^GT[ACGT]AC
MflI 374 GGATCT [GA]^GATC[CT]
MroI 371 TCCGGA T^CCGGA
MspA1I 593 CCGCGG C[AC]G^C[GT]G
NarI 325 GGCGCC GG^CGCC
NspI 257 ACATGC [GA]CATG^[CT]
PaeR7I 238 CTCGAG C^TCGAG
PflMI 441 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 174 GTAC GT^AC
SacII 593 CCGCGG CCGC^GG
SfoI 325 GGCGCC GGC^GCC
Tsp509I 464 AATT ^AATT
TspRI 262 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 441 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 238 CTCGAG C^TCGAG
XhoII 374 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 214 GGCGTC G[GA]^CG[CT]C
325 GGCGCC
AluI 394 AGCT AG^CT
452 AGCT
AlwNI 398 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
442 CAGACCCTG
BanII 235 GGGCTC G[GA]GC[CT]^C
597 GGGCCC
BmyI 235 GGGCTC G[AGT]GC[ACT]^C
597 GGGCCC
BpmI 448 CTGGAG CTGGAG(16/14)
454 CTGGAG
BsaHI 214 GGCGTC G[GA]^CG[CT]C
325 GGCGCC
BsaWI 67 ACCGGA [AT]^CCGG[AT]
371 TCCGGA
Bsp1286I 235 GGGCTC G[AGT]GC[ACT]^C
597 GGGCCC
BstNI 383 CCTGG CC^[AT]GG
447 CCTGG
EarI 640 GAAGAG CTCTTC(1/4)
646 GAAGAG
EcoRII 383 CCTGG ^CC[AT]GG
447 CCTGG
EcoT14I 314 CCAAGG C^C[AT][AT]GG
329 CCAAGG
HgaI 486 GACGC GACGC(5/10)
215 GCGTC
Hin1I 214 GGCGTC G[GA]^CG[CT]C
325 GGCGCC
HinfI 307 GACTC G^A[ACGT]TC
343 GACTC
Ksp632I 640 GAAGAG CTCTTC(1/4)
646 GAAGAG
MboII 640 GAAGA GAAGA(8/7)
646 GAAGA
MvaI 383 CCTGG CC^[AT]GG
447 CCTGG
NaeI 211 GCCGGC GCC^GGC
226 GCCGGC
NgoMIV 211 GCCGGC G^CCGGC
226 GCCGGC
PleI 307 GACTC GAGTC(4/5)
343 GACTC
SmaI 120 CCCGGG CCC^GGG
600 CCCGGG
SmlI 48 CTCAAG C^T[CT][GA]AG
238 CTCGAG
StyI 314 CCAAGG C^C[AT][AT]GG
329 CCAAGG
XmaCI 120 CCCGGG C^CCGGG
600 CCCGGG
XmaI 120 CCCGGG C^CCGGG
600 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AvaI BcnI BsaJI
BsiEI BsiYI BslI BsmFI BsoBI
BsrFI BssKI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII EaeI EagI
EclXI Eco52I FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MboI MnlI MspI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
TthHB8I