Restriction map for
pFN21AB8966
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AclI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvrII BalI BamHI BbrPI BbvCI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsgI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MamI MfeI MflI MluI MluNI
MscI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI RsrII SacI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 877 GACGTC GACGT^C
AccI 117 GTCGAC GT^[AC][GT]AC
AccIII 955 TCCGGA T^CCGGA
AflII 765 CTTAAG C^TTAAG
AlwI 379 GGATC GGATC(4/5)
AlwNI 460 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 123 ATTAAT AT^TAAT
AsnI 123 ATTAAT AT^TAAT
Asp700I 477 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 437 TGCGCA TGC^GCA
BcgI 808 GCAGGCGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 971 CTAG C^TAG
BfrI 765 CTTAAG C^TTAAG
BsaI 192 GAGACC GGTCTC(1/5)
BsaWI 955 TCCGGA [AT]^CCGG[AT]
BseAI 955 TCCGGA T^CCGGA
BsmBI 335 CGTCTC CGTCTC(1/5)
BspEI 955 TCCGGA T^CCGGA
BsrDI 866 CATTGC GCAATG(2/0)
DdeI 798 CTTAG C^T[ACGT]AG
DraII 760 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 991 GACAGCCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 683 CCGCGG C^C[GA][CT]GG
EarI 1015 GAAGAG CTCTTC(1/4)
EcoO109I 760 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 481 GAATTC G^AATTC
EcoT14I 286 CCTTGG C^C[AT][AT]GG
FspI 437 TGCGCA TGC^GCA
HincII 117 GTCGAC GT[CT]^[GA]AC
HindII 117 GTCGAC GT[CT]^[GA]AC
Ksp632I 1015 GAAGAG CTCTTC(1/4)
KspI 683 CCGCGG CCGC^GG
MaeI 971 CTAG C^TAG
MaeIII 737 GTCAC ^GT[ACGT]AC
MroI 955 TCCGGA T^CCGGA
NlaIII 585 CATG CATG^
PshAI 937 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 123 ATTAAT AT^TAAT
PvuII 460 CAGCTG CAG^CTG
SacII 683 CCGCGG CCGC^GG
SalI 117 GTCGAC G^TCGAC
SapI 1015 GAAGAGC GCTCTTC(1/4)
SmlI 765 CTTAAG C^T[CT][GA]AG
StuI 960 AGGCCT AGG^CCT
StyI 286 CCTTGG C^C[AT][AT]GG
TfiI 898 GATTC G^A[AT]TC
Tsp45I 737 GTCAC ^GT[GC]AC
XmnI 477 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 498 GTAC GT^AC
1037 GTAC
BanII 647 GAGCCC G[GA]GC[CT]^C
856 GAGCCC
BbeI 175 GGCGCC GGCGC^C
890 GGCGCC
BbsI 874 GAAGAC GAAGAC(2/6)
880 GTCTTC
BmyI 647 GAGCCC G[AGT]GC[ACT]^C
856 GAGCCC
BpuAI 874 GAAGAC GAAGAC(2/6)
880 GTCTTC
BsiEI 347 CGGCCG CG[GA][CT]^CG
932 CGGCCG
Bsp1286I 647 GAGCCC G[AGT]GC[ACT]^C
856 GAGCCC
BspMI 1055 ACCTGC ACCTGC(4/8)
555 GCAGGT
BsrI 471 ACTGG ACTGG(1/-1)
1034 CCAGT
BssHII 427 GCGCGC G^CGCGC
830 GCGCGC
BstF5I 290 GGATG GGATG(2/0)
624 GGATG
DpnII 380 GATC ^GATC
525 GATC
EagI 347 CGGCCG C^GGCCG
932 CGGCCG
EclXI 347 CGGCCG C^GGCCG
932 CGGCCG
Eco52I 347 CGGCCG C^GGCCG
932 CGGCCG
FokI 290 GGATG GGATG(9/13)
624 GGATG
HaeII 175 GGCGCC [GA]GCGC^[CT]
890 GGCGCC
KasI 175 GGCGCC G^GCGCC
890 GGCGCC
MaeII 334 ACGT A^CGT
878 ACGT
MboI 380 GATC ^GATC
525 GATC
NaeI 506 GCCGGC GCC^GGC
844 GCCGGC
NarI 175 GGCGCC GG^CGCC
890 GGCGCC
NdeII 380 GATC ^GATC
525 GATC
NgoMIV 506 GCCGGC G^CCGGC
844 GCCGGC
PstI 386 CTGCAG CTGCA^G
1057 CTGCAG
RsaI 498 GTAC GT^AC
1037 GTAC
Sau3AI 380 GATC ^GATC
525 GATC
SfoI 175 GGCGCC GGC^GCC
890 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
ApoI AvaI AvaII BanI BbvI
BcnI BglI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I EaeI
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV PleI Sau96I
ScrFI SfcI SmaI TaqI Tru9I
TseI Tsp509I TthHB8I XmaCI XmaI