Restriction map for
pFN21AE6289
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsgI BsiEI BsiHKAI BsmAI
BsmBI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstYI BstZ17I CelII CfoI ClaI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NotI NruI PacI
PaeR7I PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
RcaI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 365 TCCGGA T^CCGGA
AclI 116 AACGTT AA^CGTT
AcsI 145 AAATTT [GA]^AATT[CT]
AflII 829 CTTAAG C^TTAAG
AflIII 620 ACGTGT A^C[GA][CT]GT
AgeI 677 ACCGGT A^CCGGT
AlwI 843 GATCC GGATC(4/5)
AlwNI 658 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 271 CCTGAGG CC^T[ACGT]AGG
ApoI 145 AAATTT [GA]^AATT[CT]
AspI 78 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 216 GGACC G^G[AT]CC
BalI 665 TGGCCA TGG^CCA
BanI 807 GGCACC G^G[CT][GA]CC
BanII 568 GAGCCC G[GA]GC[CT]^C
BbsI 339 GAAGAC GAAGAC(2/6)
BfaI 913 CTAG C^TAG
BfrI 829 CTTAAG C^TTAAG
BpuAI 339 GAAGAC GAAGAC(2/6)
BseAI 365 TCCGGA T^CCGGA
BsiWI 639 CGTACG C^GTACG
BsmI 779 GAATGC GAATGC(1/-1)
Bsp1407I 238 TGTACA T^GTACA
BspEI 365 TCCGGA T^CCGGA
BsrGI 238 TGTACA T^GTACA
BstBI 742 TTCGAA TT^CGAA
BstXI 224 CCATACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 271 CCTGAGG CC^T[ACGT]AGG
CpoI 215 CGGACCG CG^G[AT]CCG
DsaI 802 CCATGG C^C[GA][CT]GG
EarI 880 GAAGAG CTCTTC(1/4)
Eco81I 271 CCTGAGG CC^T[ACGT]AGG
EcoRV 427 GATATC GAT^ATC
EcoT22I 781 ATGCAT ATGCA^T
HincII 753 GTTGAC GT[CT]^[GA]AC
HindII 753 GTTGAC GT[CT]^[GA]AC
Ksp632I 880 GAAGAG CTCTTC(1/4)
MaeI 913 CTAG C^TAG
MluNI 665 TGGCCA TGG^CCA
MroI 365 TCCGGA T^CCGGA
MscI 665 TGGCCA TGG^CCA
NcoI 802 CCATGG C^CATGG
NheI 912 GCTAGC G^CTAGC
NlaIV 807 GGCACC GG[ACGT]^[ACGT]CC
NsiI 781 ATGCAT ATGCA^T
NspV 742 TTCGAA TT^CGAA
PflFI 78 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 677 ACCGGT A^CCGGT
Psp1406I 116 AACGTT AA^CGTT
RsrII 215 CGGACCG CG^G[AT]CCG
SfcI 92 CTATAG C^T[GA][CT]AG
SfuI 742 TTCGAA TT^CGAA
SphI 644 GCATGC GCATG^C
SspBI 238 TGTACA T^GTACA
TfiI 559 GAATC G^A[AT]TC
Tsp45I 76 GTGAC ^GT[GC]AC
Tsp509I 146 AATT ^AATT
TspRI 499 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 78 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 86 CCGC CCGC(-3/-1)
543 CCGC
BbvCI 853 CCTCAGC CCTCAGC(-5/-2)
907 CCTCAGC
BcnI 179 CCCGG CC^[GC]GG
653 CCCGG
BmyI 568 GAGCCC G[AGT]GC[ACT]^C
806 GGGCAC
BsaWI 365 TCCGGA [AT]^CCGG[AT]
677 ACCGGT
BsmFI 309 GGGAC GGGAC(10/14)
521 GGGAC
Bsp1286I 568 GAGCCC G[AGT]GC[ACT]^C
806 GGGCAC
BsrFI 677 ACCGGT [GA]^CCGG[CT]
686 GCCGGT
BsrI 67 CCAGT ACTGG(1/-1)
100 CCAGT
BstF5I 825 CATCC GGATG(2/0)
720 GGATG
Cfr10I 677 ACCGGT [GA]^CCGG[CT]
686 GCCGGT
DraI 148 TTTAAA TTT^AAA
394 TTTAAA
FokI 825 CATCC GGATG(9/13)
720 GGATG
MaeII 117 ACGT A^CGT
620 ACGT
MaeIII 76 GTGAC ^GT[ACGT]AC
490 GTAAC
NciI 179 CCCGG CC^[GC]GG
653 CCCGG
NspI 174 ACATGC [GA]CATG^[CT]
644 GCATGC
PleI 78 GACTC GAGTC(4/5)
660 GACTC
PvuII 242 CAGCTG CAG^CTG
856 CAGCTG
SmlI 17 CTCAAG C^T[CT][GA]AG
829 CTTAAG
TaqI 37 TCGA T^CGA
743 TCGA
TthHB8I 37 TCGA T^CGA
743 TCGA
XcmI 508 CCAGACATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
716 CCAAGGATGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI BbvI BpmI BsaJI
BsiYI BslI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI