Restriction map for
pFN21AE5767
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BseRI BsiEI BsiWI
BsoBI BspDI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MscI NarI
NcoI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 4 GACGTC GACGT^C
AccI 949 GTAGAC GT^[AC][GT]AC
AccII 33 CGCG CG^CG
AccIII 385 TCCGGA T^CCGGA
AcyI 4 GACGTC G[GA]^CG[CT]C
AlwNI 130 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1309 ATTAAT AT^TAAT
AsnI 1309 ATTAAT AT^TAAT
Asp700I 1279 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1291 GAGCTC G[AT]GC[AT]^C
AspI 1105 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 791 GGATAC GTATCC(6/5)
BclI 1451 TGATCA T^GATCA
BcnI 913 CCCGG CC^[GC]GG
BglI 23 GCCGGCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 4 GACGTC G[GA]^CG[CT]C
BseAI 385 TCCGGA T^CCGGA
BsgI 1023 CTGCAC GTGCAG(16/14)
BsiHKAI 1291 GAGCTC G[AT]GC[AT]^C
BsmBI 54 GAGACG CGTCTC(1/5)
BsmI 659 GCATTC GAATGC(1/-1)
Bsp1407I 1402 TGTACA T^GTACA
BspEI 385 TCCGGA T^CCGGA
BspHI 1287 TCATGA T^CATGA
BspMI 547 GCAGGT ACCTGC(4/8)
BsrFI 23 GCCGGC [GA]^CCGG[CT]
BsrGI 1402 TGTACA T^GTACA
BstAPI 1060 GCAACTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 33 CGCG CG^CG
BstXI 1010 CCATTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 32 GCGC GCG^C
Cfr10I 23 GCCGGC [GA]^CCGG[CT]
DraI 1432 TTTAAA TTT^AAA
EaeI 1202 TGGCCG [CT]^GGCC[GA]
EcoRI 1137 GAATTC G^AATTC
EcoT14I 453 CCAAGG C^C[AT][AT]GG
FbaI 1451 TGATCA T^GATCA
HhaI 32 GCGC GCG^C
Hin1I 4 GACGTC G[GA]^CG[CT]C
HinP1I 32 GCGC G^CGC
MfeI 476 CAATTG C^AATTG
MroI 385 TCCGGA T^CCGGA
MslI 1113 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 476 CAATTG C^AATTG
MvnI 33 CGCG CG^CG
NaeI 23 GCCGGC GCC^GGC
NciI 913 CCCGG CC^[GC]GG
NdeI 1070 CATATG CA^TATG
NgoMIV 23 GCCGGC G^CCGGC
NheI 1413 GCTAGC G^CTAGC
PflFI 1105 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1309 ATTAAT AT^TAAT
PstI 1142 CTGCAG CTGCA^G
RcaI 1287 TCATGA T^CATGA
SacI 1291 GAGCTC GAGCT^C
SspBI 1402 TGTACA T^GTACA
StyI 453 CCAAGG C^C[AT][AT]GG
Tth111I 1105 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 540 TCTAGA T^CTAGA
XmnI 1279 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 835 GGACC G^G[AT]CC
1038 GGTCC
BanII 707 GAGCCC G[GA]GC[CT]^C
1291 GAGCTC
BmyI 707 GAGCCC G[AGT]GC[ACT]^C
1291 GAGCTC
BsaI 995 GAGACC GGTCTC(1/5)
1186 GAGACC
BsaWI 385 TCCGGA [AT]^CCGG[AT]
1189 ACCGGA
BsiYI 202 CCCCAAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1262 CCATTGAAAGG
BslI 202 CCCCAAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1262 CCATTGAAAGG
BsmFI 132 GGGAC GGGAC(10/14)
163 GTCCC
Bsp1286I 707 GAGCCC G[AGT]GC[ACT]^C
1291 GAGCTC
BssSI 51 CACGAG CACGAG(-5/-1)
728 CACGAG
FauI 688 CCCGC CCCGC(4/6)
34 GCGGG
HgaI 56 GACGC GACGC(5/10)
70 GACGC
HphI 1449 GGTGA GGTGA(8/7)
721 TCACC
MaeII 5 ACGT A^CGT
449 ACGT
MaeIII 868 GTGAC ^GT[ACGT]AC
1750 GTCAC
MspA1I 676 CAGCTG C[AC]G^C[GT]G
1000 CAGCTG
NlaIV 37 GGGGCC GG[ACGT]^[ACGT]CC
457 GGAACC
PvuII 676 CAGCTG CAG^CTG
1000 CAGCTG
SapI 1043 GAAGAGC GCTCTTC(1/4)
1511 GAAGAGC
SfaNI 544 GATGC GCATC(5/9)
181 GCATC
Tsp45I 868 GTGAC ^GT[GC]AC
1750 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BfaI BpuAI
BsaJI BsmAI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeI MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII