##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BfrI        BglI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI         1168  AAATTT               [GA]^AATT[CT]
AflIII        581  ACATGT               A^C[GA][CT]GT
AlwNI        1028  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI         1168  AAATTT               [GA]^AATT[CT]
AvrII        1038  CCTAGG               C^CTAGG
BalI          815  TGGCCA               TGG^CCA
BbsI          253  GTCTTC               GAAGAC(2/6)
BciVI        1328  GTATCC               GTATCC(6/5)
BclI         1370  TGATCA               T^GATCA
BcnI          369  CCCGG                CC^[GC]GG
BglII          53  AGATCT               A^GATCT
BlnI         1038  CCTAGG               C^CTAGG
BpuAI         253  GTCTTC               GAAGAC(2/6)
BsaI          934  GAGACC               GGTCTC(1/5)
BsaWI         290  TCCGGT               [AT]^CCGG[AT]
BspLU11I      581  ACATGT               A^CATGT
BsrI           20  CCAGT                ACTGG(1/-1)
BstAPI       1027  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoNI        1033  CCTGCCCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1370  TGATCA               T^GATCA
HincII        355  GTCAAC               GT[CT]^[GA]AC
HindII        355  GTCAAC               GT[CT]^[GA]AC
MaeII        1490  ACGT                 A^CGT
MfeI         1247  CAATTG               C^AATTG
MluNI         815  TGGCCA               TGG^CCA
MscI          815  TGGCCA               TGG^CCA
MunI         1247  CAATTG               C^AATTG
NciI          369  CCCGG                CC^[GC]GG
PflMI         771  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1513  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         260  CAGCTG               CAG^CTG
ScaI         1438  AGTACT               AGT^ACT
SphI          455  GCATGC               GCATG^C
TaqI          887  TCGA                 T^CGA
TthHB8I       887  TCGA                 T^CGA
Van91I        771  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BseRI        1405  GAGGAG               GAGGAG(10/8)
             1522  GAGGAG              
BsgI          469  CTGCAC               GTGCAG(16/14)
             1025  GTGCAG              
BsmFI         634  GGGAC                GGGAC(10/14)
             1513  GGGAC               
DraII         404  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1513  GGGACCT             
DraIII        472  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1480  CACACTGTG           
EaeI          534  CGGCCA               [CT]^GGCC[GA]
              815  TGGCCA              
EcoO109I      404  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1513  GGGACCT             
FauI          938  CCCGC                CCCGC(4/6)
             1100  CCCGC               
MseI          587  TTAA                 T^TAA
             1172  TTAA                
MspA1I        109  CCGCTG               C[AC]G^C[GT]G
              260  CAGCTG              
NcoI           78  CCATGG               C^CATGG
             1121  CCATGG              
SmlI            7  CTCAAG               C^T[CT][GA]AG
             1159  CTTGAG              
Tru9I         587  TTAA                 T^TAA
             1172  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BanII       BbvI        BfaI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspMI       BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PstI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
Tsp45I      Tsp509I     TspRI       XhoII