Restriction map for
pFN21AE6637
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BlnI BpuAI
BsaAI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MaeII MfeI MluI MluNI MroI
MscI MseI MspA1I MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 339 CCGC CCGC(-3/-1)
AlwI 618 GATCC GGATC(4/5)
Asp700I 31 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 211 GGCGCC G^G[CT][GA]CC
BanII 594 GGGCTC G[GA]GC[CT]^C
BbeI 211 GGCGCC GGCGC^C
BbvI 18 GCAGC GCAGC(8/12)
BclI 108 TGATCA T^GATCA
BcnI 73 CCGGG CC^[GC]GG
BglII 419 AGATCT A^GATCT
BlpI 13 GCTGAGC GC^T[ACGT]AGC
BmyI 594 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 13 GCTGAGC GC^T[ACGT]AGC
BsaI 4 GAGACC GGTCTC(1/5)
BsaWI 188 TCCGGT [AT]^CCGG[AT]
BsmBI 325 CGTCTC CGTCTC(1/5)
Bsp1286I 594 GGGCTC G[AGT]GC[ACT]^C
BspDI 624 ATCGAT AT^CGAT
BsrI 411 CCAGT ACTGG(1/-1)
Cac8I 444 GCAAGC GC[ACGT]^[ACGT]GC
CelII 13 GCTGAGC GC^T[ACGT]AGC
ClaI 624 ATCGAT AT^CGAT
EcoRV 524 GATATC GAT^ATC
FbaI 108 TGATCA T^GATCA
Fnu4HI 18 GCAGC GC^[ACGT]GC
HaeII 211 GGCGCC [GA]GCGC^[CT]
HphI 143 TCACC GGTGA(8/7)
ItaI 18 GCAGC GC^[ACGT]GC
KasI 211 GGCGCC G^GCGCC
MaeIII 554 GTGAC ^GT[ACGT]AC
MslI 45 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 18 GCAGCAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 211 GGCGCC GG^CGCC
NciI 73 CCGGG CC^[GC]GG
PflMI 449 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 367 GAGTC GAGTC(4/5)
PpuMI 76 GGGTCCC [GA]G^G[AT]CC[CT]
SfaNI 289 GATGC GCATC(5/9)
SfoI 211 GGCGCC GGC^GCC
TaqI 625 TCGA T^CGA
TseI 18 GCAGC G^C[AT]GC
Tsp45I 554 GTGAC ^GT[GC]AC
TspRI 412 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 625 TCGA T^CGA
Van91I 449 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 31 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcyI 211 GGCGCC G[GA]^CG[CT]C
407 GACGCC
AvaII 77 GGTCC G^G[AT]CC
191 GGTCC
BpmI 262 CTGGAG CTGGAG(16/14)
294 CTGGAG
BsaBI 96 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
375 GATGACCATC
BsaHI 211 GGCGCC G[GA]^CG[CT]C
407 GACGCC
BseRI 269 GAGGAG GAGGAG(10/8)
566 GAGGAG
BsmFI 78 GTCCC GGGAC(10/14)
192 GTCCC
BstF5I 134 GGATG GGATG(2/0)
288 GGATG
BstXI 44 CCACCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
513 CCAATATCCTGG
BstYI 419 AGATCT [GA]^GATC[CT]
617 AGATCC
CfoI 171 GCGC GCG^C
212 GCGC
DraII 76 GGGTCCC [GA]G^G[ACGT]CC[CT]
496 GGGGCCT
DsaI 88 CCATGG C^C[GA][CT]GG
449 CCATGG
EcoO109I 76 GGGTCCC [GA]G^G[ACGT]CC[CT]
496 GGGGCCT
EcoT14I 88 CCATGG C^C[AT][AT]GG
449 CCATGG
FokI 134 GGATG GGATG(9/13)
288 GGATG
HaeIII 86 GGCC GG^CC
498 GGCC
HapII 73 CCGG C^CGG
189 CCGG
HgaI 407 GACGC GACGC(5/10)
324 GCGTC
HhaI 171 GCGC GCG^C
212 GCGC
Hin1I 211 GGCGCC G[GA]^CG[CT]C
407 GACGCC
HinP1I 171 GCGC G^CGC
212 GCGC
HpaII 73 CCGG C^CGG
189 CCGG
MamI 96 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
375 GATGACCATC
MboII 93 GAAGA GAAGA(8/7)
483 GAAGA
MflI 419 AGATCT [GA]^GATC[CT]
617 AGATCC
MspI 73 CCGG C^CGG
189 CCGG
NcoI 88 CCATGG C^CATGG
449 CCATGG
StyI 88 CCATGG C^C[AT][AT]GG
449 CCATGG
TfiI 627 GATTC G^A[AT]TC
635 GAATC
XhoII 419 AGATCT [GA]^GATC[CT]
617 AGATCC
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI AlwNI BsaJI BsiYI BslI
BsmAI BssKI BstNI Cfr13I DdeI
DpnII EcoRII HinfI MboI MnlI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI