Restriction map for
pFN21AE6490
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AfaI AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BciVI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsaI RsrII SacII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 881 TCCGGA T^CCGGA
AflII 247 CTTAAG C^TTAAG
ApaI 966 GGGCCC GGGCC^C
BbvCI 431 CCTCAGC CCTCAGC(-5/-2)
BclI 1646 TGATCA T^GATCA
BfrI 247 CTTAAG C^TTAAG
BsaBI 207 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 881 TCCGGA [AT]^CCGG[AT]
BseAI 881 TCCGGA T^CCGGA
BsiEI 1309 CGATCG CG[GA][CT]^CG
BspEI 881 TCCGGA T^CCGGA
BsrDI 1374 CATTGC GCAATG(2/0)
BstEII 561 GGTGACC G^GT[ACGT]ACC
BstPI 561 GGTGACC G^GT[ACGT]ACC
BstYI 857 AGATCC [GA]^GATC[CT]
EcoNI 1290 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 561 GGTGACC G^GT[ACGT]ACC
FbaI 1646 TGATCA T^GATCA
HaeII 135 GGCGCT [GA]GCGC^[CT]
HgaI 938 GCGTC GACGC(5/10)
HindIII 568 AAGCTT A^AGCTT
MamI 207 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 857 AGATCC [GA]^GATC[CT]
MroI 881 TCCGGA T^CCGGA
NspI 456 ACATGC [GA]CATG^[CT]
PflMI 5 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1309 CGATCG CGAT^CG
SacI 532 GAGCTC GAGCT^C
SexAI 728 ACCTGGT A^CC[AT]GGT
SfiI 18 GGCCCTGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 247 CTTAAG C^T[CT][GA]AG
StuI 104 AGGCCT AGG^CCT
TfiI 1619 GATTC G^A[AT]TC
Van91I 5 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 122 CCATCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 857 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 114 CGCG CG^CG
294 CGCG
Alw44I 147 GTGCAC G^TGCAC
1416 GTGCAC
AlwI 858 GATCC GGATC(4/5)
413 GGATC
AlwNI 16 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1735 CAGTGCCTG
ApaLI 147 GTGCAC G^TGCAC
1416 GTGCAC
AvaI 1034 CCCGGG C^[CT]CG[GA]G
1610 CCCGGG
BanI 444 GGTGCC G^G[CT][GA]CC
715 GGCACC
BanII 532 GAGCTC G[GA]GC[CT]^C
966 GGGCCC
BbsI 1230 GAAGAC GAAGAC(2/6)
1573 GTCTTC
BfaI 1421 CTAG C^TAG
1459 CTAG
BglI 19 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1462 GCCTCCATGGC
BpmI 535 CTCCAG CTGGAG(16/14)
1367 CTCCAG
BpuAI 1230 GAAGAC GAAGAC(2/6)
1573 GTCTTC
BsoBI 1034 CCCGGG C^[CT]CG[GA]G
1610 CCCGGG
BspMI 7 ACCTGC ACCTGC(4/8)
311 ACCTGC
BstAPI 954 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1353 GCAGAGTGTGC
BstUI 114 CGCG CG^CG
294 CGCG
BstXI 290 CCATCGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1523 CCACCTCCCTGG
CfoI 113 GCGC GCG^C
136 GCGC
DraIII 651 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1410 CACCGAGTG
FauI 366 CCCGC CCCGC(4/6)
1557 CCCGC
HhaI 113 GCGC GCG^C
136 GCGC
HinP1I 113 GCGC G^CGC
136 GCGC
MaeI 1421 CTAG C^TAG
1459 CTAG
MseI 190 TTAA T^TAA
248 TTAA
MspA1I 472 CAGCTG C[AC]G^C[GT]G
1668 CAGCTG
MvnI 114 CGCG CG^CG
294 CGCG
NcoI 818 CCATGG C^CATGG
1466 CCATGG
PleI 253 GACTC GAGTC(4/5)
1640 GAGTC
PpuMI 1178 AGGACCC [GA]G^G[AT]CC[CT]
1586 GGGACCT
PstI 853 CTGCAG CTGCA^G
1351 CTGCAG
PvuII 472 CAGCTG CAG^CTG
1668 CAGCTG
SapI 138 GCTCTTC GCTCTTC(1/4)
756 GCTCTTC
SfcI 853 CTGCAG C^T[GA][CT]AG
1351 CTGCAG
SmaI 1034 CCCGGG CCC^GGG
1610 CCCGGG
Tru9I 190 TTAA T^TAA
248 TTAA
Tsp509I 1066 AATT ^AATT
1137 AATT
XmaCI 1034 CCCGGG C^CCGGG
1610 CCCGGG
XmaI 1034 CCCGGG C^CCGGG
1610 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BbvI BcgI BcnI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I