Restriction map for
pFN21AE2411
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BbeI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BsaI BsaWI BseAI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PleI PmeI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
StuI StyI SwaI Tth111I Van91I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AlwNI 319 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1114 ATTAAT AT^TAAT
AsnI 1114 ATTAAT AT^TAAT
AspHI 1030 GTGCTC G[AT]GC[AT]^C
BamHI 1470 GGATCC G^GATCC
BanI 1487 GGTGCC G^G[CT][GA]CC
BanII 16 GAGCCC G[GA]GC[CT]^C
BbsI 1657 GTCTTC GAAGAC(2/6)
BclI 161 TGATCA T^GATCA
BfaI 1360 CTAG C^TAG
BglI 191 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1657 GTCTTC GAAGAC(2/6)
BsaBI 1323 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 647 CTGCAC GTGCAG(16/14)
BsiEI 1101 CGACCG CG[GA][CT]^CG
BsiHKAI 1030 GTGCTC G[AT]GC[AT]^C
BsiWI 308 CGTACG C^GTACG
BssSI 1179 CACGAG CACGAG(-5/-1)
BstAPI 414 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1470 GGATCC [GA]^GATC[CT]
DraII 437 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 60 CCGTGG C^C[GA][CT]GG
EcoO109I 437 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 161 TGATCA T^GATCA
MaeI 1360 CTAG C^TAG
MamI 1323 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1470 GGATCC [GA]^GATC[CT]
NspI 797 GCATGC [GA]CATG^[CT]
PaeR7I 748 CTCGAG C^TCGAG
PpuMI 437 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 465 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1114 ATTAAT AT^TAAT
SphI 797 GCATGC GCATG^C
SspI 527 AATATT AAT^ATT
TaqI 749 TCGA T^CGA
TthHB8I 749 TCGA T^CGA
XhoI 748 CTCGAG C^TCGAG
XhoII 1470 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 10 CCTGAGG CC^T[ACGT]AGG
1465 CCTCAGG
AvaI 706 CTCGGG C^[CT]CG[GA]G
748 CTCGAG
BbrPI 331 CACGTG CAC^GTG
1078 CACGTG
BcnI 39 CCCGG CC^[GC]GG
954 CCGGG
BsaAI 331 CACGTG [CT]AC^GT[GA]
1078 CACGTG
BsmFI 744 GTCCC GGGAC(10/14)
922 GTCCC
BsoBI 706 CTCGGG C^[CT]CG[GA]G
748 CTCGAG
Bsp1407I 914 TGTACA T^GTACA
1333 TGTACA
BsrGI 914 TGTACA T^GTACA
1333 TGTACA
Bsu36I 10 CCTGAGG CC^T[ACGT]AGG
1465 CCTCAGG
Eco81I 10 CCTGAGG CC^T[ACGT]AGG
1465 CCTCAGG
EcoRI 1452 GAATTC G^AATTC
1703 GAATTC
HinfI 266 GATTC G^A[ACGT]TC
1691 GATTC
HphI 636 GGTGA GGTGA(8/7)
1479 TCACC
MslI 798 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
906 CATCATTGTG
NciI 39 CCCGG CC^[GC]GG
954 CCGGG
PmaCI 331 CACGTG CAC^GTG
1078 CACGTG
PmlI 331 CACGTG CAC^GTG
1078 CACGTG
PvuII 145 CAGCTG CAG^CTG
1621 CAGCTG
SbfI 756 CCTGCAGG CCTGCA^GG
925 CCTGCAGG
SmlI 52 CTTGAG C^T[CT][GA]AG
748 CTCGAG
Sse8387I 756 CCTGCAGG CCTGCA^GG
925 CCTGCAGG
SspBI 914 TGTACA T^GTACA
1333 TGTACA
TfiI 266 GATTC G^A[AT]TC
1691 GATTC
XcmI 558 CCAGATCGTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1613 CCAGGAAACAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BalI BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI