Restriction map for
pFN21AE9547
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
BbrPI BbvCI BcgI BfrI BglII
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 694 GACGTC GACGT^C
AcsI 1079 AAATTC [GA]^AATT[CT]
AflIII 308 ACGTGT A^C[GA][CT]GT
Alw44I 965 GTGCAC G^TGCAC
ApaI 137 GGGCCC GGGCC^C
ApaLI 965 GTGCAC G^TGCAC
ApoI 1079 AAATTC [GA]^AATT[CT]
Asp700I 1404 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 694 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1574 CCCGGG C^[CT]CG[GA]G
AvrII 1419 CCTAGG C^CTAGG
BalI 1619 TGGCCA TGG^CCA
BamHI 196 GGATCC G^GATCC
BbeI 1540 GGCGCC GGCGC^C
BbsI 700 GTCTTC GAAGAC(2/6)
BciVI 553 GGATAC GTATCC(6/5)
BglI 1373 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1419 CCTAGG C^CTAGG
BlpI 1333 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1333 GCTGAGC GC^T[ACGT]AGC
BpuAI 700 GTCTTC GAAGAC(2/6)
BsaAI 307 TACGTG [CT]AC^GT[GA]
BsaWI 1412 TCCGGT [AT]^CCGG[AT]
BsmI 472 GAATGC GAATGC(1/-1)
BsoBI 1574 CCCGGG C^[CT]CG[GA]G
Bsp1407I 542 TGTACA T^GTACA
BspHI 1188 TCATGA T^CATGA
BspMI 908 ACCTGC ACCTGC(4/8)
BsrBI 780 CCGCTC CCGCTC(-3/-3)
BsrGI 542 TGTACA T^GTACA
BssSI 64 CACGAG CACGAG(-5/-1)
BstBI 608 TTCGAA TT^CGAA
BstXI 1622 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1333 GCTGAGC GC^T[ACGT]AGC
DraIII 981 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1619 TGGCCA [CT]^GGCC[GA]
HgaI 59 GCGTC GACGC(5/10)
KasI 1540 GGCGCC G^GCGCC
MluNI 1619 TGGCCA TGG^CCA
MscI 1619 TGGCCA TGG^CCA
MseI 1490 TTAA T^TAA
MslI 636 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 535 CAGCTG C[AC]G^C[GT]G
NarI 1540 GGCGCC GG^CGCC
NheI 1365 GCTAGC G^CTAGC
NspI 244 GCATGT [GA]CATG^[CT]
NspV 608 TTCGAA TT^CGAA
PflFI 694 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1032 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 535 CAGCTG CAG^CTG
RcaI 1188 TCATGA T^CATGA
SfoI 1540 GGCGCC GGC^GCC
SfuI 608 TTCGAA TT^CGAA
SmaI 1574 CCCGGG CCC^GGG
SspBI 542 TGTACA T^GTACA
TfiI 1638 GATTC G^A[AT]TC
Tru9I 1490 TTAA T^TAA
Tsp45I 1612 GTCAC ^GT[GC]AC
Tth111I 694 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1574 CCCGGG C^CCGGG
XmaI 1574 CCCGGG C^CCGGG
XmnI 1404 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 42 CGCG CG^CG
626 CGCG
BanI 814 GGTGCC G^G[CT][GA]CC
1540 GGCGCC
BclI 300 TGATCA T^GATCA
1218 TGATCA
BcnI 1574 CCCGG CC^[GC]GG
1575 CCGGG
BpmI 865 CTGGAG CTGGAG(16/14)
973 CTGGAG
BseRI 595 CTCCTC GAGGAG(10/8)
1633 GAGGAG
BsgI 621 CTGCAC GTGCAG(16/14)
1596 GTGCAG
BsmAI 254 GAGAC GTCTC(1/5)
340 GTCTC
BstUI 42 CGCG CG^CG
626 CGCG
BstYI 196 GGATCC [GA]^GATC[CT]
1193 AGATCC
FauI 1370 CCCGC CCCGC(4/6)
43 GCGGG
FbaI 300 TGATCA T^GATCA
1218 TGATCA
HaeII 1456 GGCGCT [GA]GCGC^[CT]
1540 GGCGCC
HapII 1413 CCGG C^CGG
1575 CCGG
HincII 525 GTCAAC GT[CT]^[GA]AC
1263 GTTGAC
HindII 525 GTCAAC GT[CT]^[GA]AC
1263 GTTGAC
HindIII 1087 AAGCTT A^AGCTT
1465 AAGCTT
HpaII 1413 CCGG C^CGG
1575 CCGG
MflI 196 GGATCC [GA]^GATC[CT]
1193 AGATCC
MspI 1413 CCGG C^CGG
1575 CCGG
MvnI 42 CGCG CG^CG
626 CGCG
NciI 1574 CCCGG CC^[GC]GG
1575 CCGGG
PstI 1383 CTGCAG CTGCA^G
1477 CTGCAG
SacI 384 GAGCTC GAGCT^C
1272 GAGCTC
SfaNI 1041 GATGC GCATC(5/9)
80 GCATC
StuI 724 AGGCCT AGG^CCT
843 AGGCCT
TaqI 13 TCGA T^CGA
609 TCGA
TthHB8I 13 TCGA T^CGA
609 TCGA
XhoII 196 GGATCC [GA]^GATC[CT]
1193 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BfaI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI Hin1I
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI StyI
TseI Tsp509I TspRI