Restriction map for
pFN21AE2059
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Aor51HI AscI AseI AsnI AspI
AviII BbeI BciVI BclI BfrI
BglI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI MfeI MluI MslI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 794 GGTACC G^GTACC
AccIII 1590 TCCGGA T^CCGGA
AcsI 722 AAATTT [GA]^AATT[CT]
AcyI 811 GGCGTC G[GA]^CG[CT]C
AflIII 1850 ACACGT A^C[GA][CT]GT
AhdI 37 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 469 GTGCAC G^TGCAC
AocI 172 CCTCAGG CC^T[ACGT]AGG
ApaI 643 GGGCCC GGGCC^C
ApaLI 469 GTGCAC G^TGCAC
ApoI 722 AAATTT [GA]^AATT[CT]
Asp700I 720 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 794 GGTACC G^GTACC
AspEI 37 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1660 CCTAGG C^CTAGG
BamHI 845 GGATCC G^GATCC
BbrPI 1872 CACGTG CAC^GTG
BbsI 1866 GAAGAC GAAGAC(2/6)
BlnI 1660 CCTAGG C^CTAGG
BpuAI 1866 GAAGAC GAAGAC(2/6)
BsaBI 1053 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 811 GGCGTC G[GA]^CG[CT]C
BseAI 1590 TCCGGA T^CCGGA
BsmBI 747 CGTCTC CGTCTC(1/5)
BsmI 1518 GAATGC GAATGC(1/-1)
BspEI 1590 TCCGGA T^CCGGA
BsrBI 214 GAGCGG CCGCTC(-3/-3)
BsrFI 518 ACCGGC [GA]^CCGG[CT]
BssSI 1177 CACGAG CACGAG(-5/-1)
BstAPI 2036 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 172 CCTCAGG CC^T[ACGT]AGG
Cfr10I 518 ACCGGC [GA]^CCGG[CT]
DraI 1621 TTTAAA TTT^AAA
DraIII 1954 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 37 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 699 GAAGAG CTCTTC(1/4)
Eco81I 172 CCTCAGG CC^T[ACGT]AGG
HaeII 2000 AGCGCC [GA]GCGC^[CT]
Hin1I 811 GGCGTC G[GA]^CG[CT]C
KpnI 794 GGTACC GGTAC^C
Ksp632I 699 GAAGAG CTCTTC(1/4)
MamI 1053 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1590 TCCGGA T^CCGGA
NcoI 647 CCATGG C^CATGG
NheI 1137 GCTAGC G^CTAGC
NspI 1364 GCATGT [GA]CATG^[CT]
PmaCI 1872 CACGTG CAC^GTG
PmlI 1872 CACGTG CAC^GTG
PvuII 106 CAGCTG CAG^CTG
SmlI 613 CTCAAG C^T[CT][GA]AG
SspI 1617 AATATT AAT^ATT
StuI 1220 AGGCCT AGG^CCT
XmnI 720 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaI 1039 CCCGAG C^[CT]CG[GA]G
1563 CCCGAG
BalI 2 TGGCCA TGG^CCA
23 TGGCCA
BanI 31 GGCACC G^G[CT][GA]CC
794 GGTACC
BcgI 1179 CGAGGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
364 GCAGATGACTCG
BfaI 1138 CTAG C^TAG
1661 CTAG
BglII 263 AGATCT A^GATCT
2012 AGATCT
BlpI 984 GCTCAGC GC^T[ACGT]AGC
1071 GCTCAGC
Bpu1102I 984 GCTCAGC GC^T[ACGT]AGC
1071 GCTCAGC
BsaAI 757 TACGTG [CT]AC^GT[GA]
1872 CACGTG
BsaI 325 GAGACC GGTCTC(1/5)
1837 GAGACC
BsaWI 492 TCCGGT [AT]^CCGG[AT]
1590 TCCGGA
BsiEI 279 CGGCCG CG[GA][CT]^CG
990 CGGCCG
BsoBI 1039 CCCGAG C^[CT]CG[GA]G
1563 CCCGAG
BsrDI 1185 CATTGC GCAATG(2/0)
1212 CATTGC
BstXI 647 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1077 CCAGACCCCTGG
CelII 984 GCTCAGC GC^T[ACGT]AGC
1071 GCTCAGC
EagI 279 CGGCCG C^GGCCG
990 CGGCCG
EclXI 279 CGGCCG C^GGCCG
990 CGGCCG
Eco52I 279 CGGCCG C^GGCCG
990 CGGCCG
HincII 814 GTCAAC GT[CT]^[GA]AC
1741 GTCAAC
HindII 814 GTCAAC GT[CT]^[GA]AC
1741 GTCAAC
KspI 669 CCGCGG CCGC^GG
1972 CCGCGG
MaeI 1138 CTAG C^TAG
1661 CTAG
MluNI 2 TGGCCA TGG^CCA
23 TGGCCA
MscI 2 TGGCCA TGG^CCA
23 TGGCCA
SacII 669 CCGCGG CCGC^GG
1972 CCGCGG
SfcI 1170 CTACAG C^T[GA][CT]AG
1703 CTATAG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvCI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII