Restriction map for
pFN21AE5559
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AviII BbeI BbrPI BbvCI BclI
BfrI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AspI 580 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 725 TGGCCA TGG^CCA
BamHI 547 GGATCC G^GATCC
BbsI 260 GTCTTC GAAGAC(2/6)
BcgI 27 GCAGAACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 80 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 311 AGATCT A^GATCT
BlpI 1140 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1140 GCTGAGC GC^T[ACGT]AGC
BpuAI 260 GTCTTC GAAGAC(2/6)
BsaAI 574 TACGTG [CT]AC^GT[GA]
BsaI 868 GGTCTC GGTCTC(1/5)
BsmI 1200 GCATTC GAATGC(1/-1)
BspMI 3 GCAGGT ACCTGC(4/8)
BssHII 1127 GCGCGC G^CGCGC
CelII 1140 GCTGAGC GC^T[ACGT]AGC
FseI 101 GGCCGGCC GGCCGG^CC
HaeII 186 AGCGCC [GA]GCGC^[CT]
HphI 1011 TCACC GGTGA(8/7)
MaeII 575 ACGT A^CGT
MluNI 725 TGGCCA TGG^CCA
MscI 725 TGGCCA TGG^CCA
MseI 514 TTAA T^TAA
NcoI 722 CCATGG C^CATGG
PflFI 580 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 657 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 896 GAGTC GAGTC(4/5)
PshAI 642 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 698 CTGCAG CTGCA^G
SacI 814 GAGCTC GAGCT^C
SfaNI 801 GATGC GCATC(5/9)
SmaI 82 CCCGGG CCC^GGG
StuI 713 AGGCCT AGG^CCT
TaqI 1096 TCGA T^CGA
Tru9I 514 TTAA T^TAA
Tth111I 580 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1096 TCGA T^CGA
Van91I 657 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 608 CCAACTGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 82 CCCGGG C^CCGGG
XmaI 82 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 286 GGTACC G^GTACC
851 GGTACC
AccII 15 CGCG CG^CG
1128 CGCG
AlwNI 658 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1135 CAGGTGCTG
ApaI 78 GGGCCC GGGCC^C
197 GGGCCC
Asp718I 286 GGTACC G^GTACC
851 GGTACC
AvaI 82 CCCGGG C^[CT]CG[GA]G
158 CCCGAG
BanI 286 GGTACC G^G[CT][GA]CC
851 GGTACC
BciVI 571 GGATAC GTATCC(6/5)
268 GTATCC
BpmI 562 CTCCAG CTGGAG(16/14)
749 CTCCAG
BseRI 447 GAGGAG GAGGAG(10/8)
622 GAGGAG
BsmFI 679 GGGAC GGGAC(10/14)
898 GTCCC
BsoBI 82 CCCGGG C^[CT]CG[GA]G
158 CCCGAG
BstUI 15 CGCG CG^CG
1128 CGCG
BstXI 822 CCACAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
983 CCAAAGTCATGG
BstYI 311 AGATCT [GA]^GATC[CT]
547 GGATCC
DsaI 722 CCATGG C^C[GA][CT]GG
1028 CCGTGG
KpnI 286 GGTACC GGTAC^C
851 GGTACC
MaeIII 295 GTCAC ^GT[ACGT]AC
844 GTCAC
MflI 311 AGATCT [GA]^GATC[CT]
547 GGATCC
MslI 225 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
683 CATCCCGATG
MvnI 15 CGCG CG^CG
1128 CGCG
NaeI 102 GCCGGC GCC^GGC
106 GCCGGC
NgoMIV 102 GCCGGC G^CCGGC
106 GCCGGC
SmlI 430 CTCAAG C^T[CT][GA]AG
940 CTCAAG
TfiI 123 GAATC G^A[AT]TC
744 GATTC
Tsp45I 295 GTCAC ^GT[GC]AC
844 GTCAC
XhoII 311 AGATCT [GA]^GATC[CT]
547 GGATCC
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII AvrII BanII BbvI BcnI
BfaI BlnI BmyI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfcI StyI TseI
TspRI