Restriction map for
pFN21AE3754
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI NaeI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 523 GTCTAC GT^[AC][GT]AC
AccII 72 CGCG CG^CG
AcsI 580 GAATTC [GA]^AATT[CT]
AcyI 32 GGCGCC G[GA]^CG[CT]C
AfaI 489 GTAC GT^AC
AlwNI 587 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 580 GAATTC [GA]^AATT[CT]
BbeI 32 GGCGCC GGCGC^C
BfaI 262 CTAG C^TAG
BglI 589 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 32 GGCGCC G[GA]^CG[CT]C
BspMI 370 GCAGGT ACCTGC(4/8)
BsrBI 67 CCGCTC CCGCTC(-3/-3)
BstUI 72 CGCG CG^CG
BstXI 630 CCAGGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 585 CTCAG C^T[ACGT]AG
DsaI 119 CCGTGG C^C[GA][CT]GG
EcoNI 624 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 580 GAATTC G^AATTC
FauI 66 CCCGC CCCGC(4/6)
Hin1I 32 GGCGCC G[GA]^CG[CT]C
KasI 32 GGCGCC G^GCGCC
MaeI 262 CTAG C^TAG
MaeIII 463 GTCAC ^GT[ACGT]AC
MslI 502 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 72 CGCG CG^CG
NarI 32 GGCGCC GG^CGCC
PflMI 343 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 731 GACTC GAGTC(4/5)
RsaI 489 GTAC GT^AC
SapI 311 GAAGAGC GCTCTTC(1/4)
SfaNI 142 GCATC GCATC(5/9)
SfoI 32 GGCGCC GGC^GCC
SmaI 467 CCCGGG CCC^GGG
SmlI 700 CTCAAG C^T[CT][GA]AG
SpeI 261 ACTAGT A^CTAGT
Tsp45I 463 GTCAC ^GT[GC]AC
Tsp509I 581 AATT ^AATT
Van91I 343 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 467 CCCGGG C^CCGGG
XmaI 467 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 281 GTGCAC G^TGCAC
499 GTGCAC
ApaI 610 GGGCCC GGGCC^C
692 GGGCCC
ApaLI 281 GTGCAC G^TGCAC
499 GTGCAC
AspHI 281 GTGCAC G[AT]GC[AT]^C
499 GTGCAC
AvaI 467 CCCGGG C^[CT]CG[GA]G
607 CTCGGG
BamHI 237 GGATCC G^GATCC
674 GGATCC
BanI 32 GGCGCC G^G[CT][GA]CC
49 GGCACC
BpmI 420 CTCCAG CTGGAG(16/14)
574 CTGGAG
BseRI 172 GAGGAG GAGGAG(10/8)
187 GAGGAG
BsiHKAI 281 GTGCAC G[AT]GC[AT]^C
499 GTGCAC
BsmFI 382 GGGAC GGGAC(10/14)
64 GTCCC
BsoBI 467 CCCGGG C^[CT]CG[GA]G
607 CTCGGG
BstF5I 340 CATCC GGATG(2/0)
549 GGATG
BstYI 237 GGATCC [GA]^GATC[CT]
674 GGATCC
EarI 733 CTCTTC CTCTTC(1/4)
311 GAAGAG
FokI 340 CATCC GGATG(9/13)
549 GGATG
HaeII 32 GGCGCC [GA]GCGC^[CT]
90 GGCGCT
HphI 464 TCACC GGTGA(8/7)
719 TCACC
Ksp632I 733 CTCTTC CTCTTC(1/4)
311 GAAGAG
MflI 237 GGATCC [GA]^GATC[CT]
674 GGATCC
PpuMI 197 AGGACCC [GA]G^G[AT]CC[CT]
661 GGGTCCT
TaqI 273 TCGA T^CGA
309 TCGA
TfiI 213 GAATC G^A[AT]TC
410 GAATC
TspRI 386 CAGTG CA[GC]TG[ACGT][ACGT]^
591 CACTG
TthHB8I 273 TCGA T^CGA
309 TCGA
XhoII 237 GGATCC [GA]^GATC[CT]
674 GGATCC
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI TseI