Restriction map for
pFN21AE6375
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# NO CUT ENZYMES ( 127 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbeI BbvCI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmFI
BsmI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MaeI MfeI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmeI PpuMI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StyI SwaI
TfiI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 1068 GTAC GT^AC
AhdI 1010 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1103 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 430 CCTCAGG CC^T[ACGT]AGG
AspEI 1010 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 413 GGTCC G^G[AT]CC
BalI 687 TGGCCA TGG^CCA
BanII 211 GAGCTC G[GA]GC[CT]^C
BbrPI 780 CACGTG CAC^GTG
BcgI 1151 CGAAGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 335 GGATAC GTATCC(6/5)
BsaAI 780 CACGTG [CT]AC^GT[GA]
BsaBI 651 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1117 CGTCTC CGTCTC(1/5)
Bsp1407I 1067 TGTACA T^GTACA
BspHI 440 TCATGA T^CATGA
BsrFI 325 ACCGGC [GA]^CCGG[CT]
BsrGI 1067 TGTACA T^GTACA
BssHII 1096 GCGCGC G^CGCGC
BstF5I 1050 CATCC GGATG(2/0)
BstYI 395 GGATCT [GA]^GATC[CT]
Bsu36I 430 CCTCAGG CC^T[ACGT]AGG
Cfr10I 325 ACCGGC [GA]^CCGG[CT]
Eam1105I 1010 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 430 CCTCAGG CC^T[ACGT]AGG
FokI 1050 CATCC GGATG(9/13)
HaeII 361 AGCGCC [GA]GCGC^[CT]
HindIII 1140 AAGCTT A^AGCTT
MamI 651 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 395 GGATCT [GA]^GATC[CT]
MluNI 687 TGGCCA TGG^CCA
MscI 687 TGGCCA TGG^CCA
MseI 148 TTAA T^TAA
PflFI 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 780 CACGTG CAC^GTG
PmlI 780 CACGTG CAC^GTG
PshAI 117 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 440 TCATGA T^CATGA
RsaI 1068 GTAC GT^AC
SacI 211 GAGCTC GAGCT^C
SapI 735 GCTCTTC GCTCTTC(1/4)
SexAI 380 ACCAGGT A^CC[AT]GGT
SfaNI 371 GCATC GCATC(5/9)
SmlI 208 CTTGAG C^T[CT][GA]AG
SspBI 1067 TGTACA T^GTACA
SspI 196 AATATT AAT^ATT
Tru9I 148 TTAA T^TAA
Tth111I 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 710 CCATCGGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 395 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AatII 613 GACGTC GACGT^C
1036 GACGTC
AcyI 613 GACGTC G[GA]^CG[CT]C
1036 GACGTC
AlwI 351 GATCC GGATC(4/5)
395 GGATC
AvaI 330 CCCGAG C^[CT]CG[GA]G
1012 CTCGGG
BcnI 920 CCGGG CC^[GC]GG
1081 CCGGG
BsaHI 613 GACGTC G[GA]^CG[CT]C
1036 GACGTC
BsaI 992 GAGACC GGTCTC(1/5)
1077 GAGACC
BsgI 628 CTGCAC GTGCAG(16/14)
661 GTGCAG
BsoBI 330 CCCGAG C^[CT]CG[GA]G
1012 CTCGGG
BstXI 381 CCAGGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
752 CCATGATCGTGG
Cfr13I 328 GGCCC G^G[ACGT]CC
413 GGTCC
EaeI 139 CGGCCA [CT]^GGCC[GA]
687 TGGCCA
EarI 736 CTCTTC CTCTTC(1/4)
796 CTCTTC
HgaI 978 GACGC GACGC(5/10)
68 GCGTC
Hin1I 613 GACGTC G[GA]^CG[CT]C
1036 GACGTC
HinfI 61 GAGTC G^A[ACGT]TC
1010 GACTC
Ksp632I 736 CTCTTC CTCTTC(1/4)
796 CTCTTC
MslI 654 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
753 CATGATCGTG
NciI 920 CCGGG CC^[GC]GG
1081 CCGGG
PleI 1010 GACTC GAGTC(4/5)
61 GAGTC
Sau96I 328 GGCCC G^G[ACGT]CC
413 GGTCC
StuI 851 AGGCCT AGG^CCT
1154 AGGCCT
Tsp509I 10 AATT ^AATT
445 AATT
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AspHI BanI
BbsI BbvI BclI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI DdeI
DpnII EcoRII FbaI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PstI Sau3AI
ScrFI SfcI TaqI TseI Tsp45I
TspRI TthHB8I