Restriction map for
pFN21AE6067
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HapII HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeII MamI MflI MluI MroI
MseI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 310 GACGCC G[GA]^CG[CT]C
AfaI 204 GTAC GT^AC
AflIII 745 ACATGT A^C[GA][CT]GT
AlwNI 26 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 732 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 112 GTGCTC G[AT]GC[AT]^C
BanI 651 GGCACC G^G[CT][GA]CC
BanII 181 GAGCCC G[GA]GC[CT]^C
BclI 866 TGATCA T^GATCA
BfaI 76 CTAG C^TAG
BglI 637 GCCTTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 594 CTCCAG CTGGAG(16/14)
BsaHI 310 GACGCC G[GA]^CG[CT]C
BsiHKAI 112 GTGCTC G[AT]GC[AT]^C
BsmAI 278 GTCTC GTCTC(1/5)
BsmBI 277 CGTCTC CGTCTC(1/5)
BspHI 857 TCATGA T^CATGA
BspLU11I 745 ACATGT A^CATGT
BsrBI 711 CCGCTC CCGCTC(-3/-3)
BsrDI 265 GCAATG GCAATG(2/0)
CfoI 923 GCGC GCG^C
DraIII 502 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 377 CCATGG C^C[GA][CT]GG
FauI 428 CCCGC CCCGC(4/6)
FbaI 866 TGATCA T^GATCA
HaeII 922 GGCGCT [GA]GCGC^[CT]
HhaI 923 GCGC GCG^C
Hin1I 310 GACGCC G[GA]^CG[CT]C
HinP1I 923 GCGC G^CGC
MaeI 76 CTAG C^TAG
MfeI 140 CAATTG C^AATTG
MspA1I 47 CCGCTG C[AC]G^C[GT]G
MunI 140 CAATTG C^AATTG
NcoI 377 CCATGG C^CATGG
NspI 745 ACATGT [GA]CATG^[CT]
PshAI 786 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 857 TCATGA T^CATGA
RsaI 204 GTAC GT^AC
StuI 734 AGGCCT AGG^CCT
TfiI 823 GAATC G^A[AT]TC
XcmI 339 CCACCTTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 732 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 35 GGACC G^G[AT]CC
44 GGACC
BalI 398 TGGCCA TGG^CCA
509 TGGCCA
BbsI 661 GTCTTC GAAGAC(2/6)
793 GTCTTC
BbvCI 153 CCTCAGC CCTCAGC(-5/-2)
718 GCTGAGG
BmyI 112 GTGCTC G[AGT]GC[ACT]^C
181 GAGCCC
BpuAI 661 GTCTTC GAAGAC(2/6)
793 GTCTTC
BseRI 115 CTCCTC GAGGAG(10/8)
118 CTCCTC
Bsp1286I 112 GTGCTC G[AGT]GC[ACT]^C
181 GAGCCC
BsrI 69 ACTGG ACTGG(1/-1)
100 CCAGT
Cac8I 313 GCCTGC GC[ACGT]^[ACGT]GC
407 GCCAGC
DpnII 867 GATC ^GATC
953 GATC
EaeI 398 TGGCCA [CT]^GGCC[GA]
509 TGGCCA
EcoT14I 377 CCATGG C^C[AT][AT]GG
656 CCTTGG
HgaI 310 GACGC GACGC(5/10)
466 GCGTC
HphI 416 TCACC GGTGA(8/7)
485 TCACC
MboI 867 GATC ^GATC
953 GATC
MluNI 398 TGGCCA TGG^CCA
509 TGGCCA
MscI 398 TGGCCA TGG^CCA
509 TGGCCA
NdeII 867 GATC ^GATC
953 GATC
PleI 392 GACTC GAGTC(4/5)
435 GAGTC
SapI 327 GCTCTTC GCTCTTC(1/4)
576 GCTCTTC
Sau3AI 867 GATC ^GATC
953 GATC
SfcI 420 CTACAG C^T[GA][CT]AG
894 CTACAG
StyI 377 CCATGG C^C[AT][AT]GG
656 CCTTGG
Tsp509I 141 AATT ^AATT
300 AATT
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
Cfr13I DdeI EarI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeIII MboII MnlI MslI MvaI
MwoI NlaIII NlaIV PflMI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI
Van91I