Restriction map for
pFN21AE6003
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeI MaeII
MfeI MflI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Van91I XbaI XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwI 519 GATCC GGATC(4/5)
AocI 873 CCTGAGG CC^T[ACGT]AGG
AspI 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 575 CTCGAG C^[CT]CG[GA]G
BbvI 66 GCAGC GCAGC(8/12)
BglI 427 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 328 CTGGAG CTGGAG(16/14)
BsaBI 594 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 575 CTCGAG C^[CT]CG[GA]G
Bsp1407I 830 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BsrGI 830 TGTACA T^GTACA
BsrI 150 ACTGG ACTGG(1/-1)
BstEII 840 GGTGACC G^GT[ACGT]ACC
BstPI 840 GGTGACC G^GT[ACGT]ACC
BstXI 110 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
CfoI 436 GCGC GCG^C
DpnII 519 GATC ^GATC
DsaI 350 CCATGG C^C[GA][CT]GG
EarI 219 CTCTTC CTCTTC(1/4)
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoO65I 840 GGTGACC G^GT[ACGT]ACC
Fnu4HI 66 GCAGC GC^[ACGT]GC
HaeII 435 GGCGCT [GA]GCGC^[CT]
HhaI 436 GCGC GCG^C
HinP1I 436 GCGC G^CGC
ItaI 66 GCAGC GC^[ACGT]GC
Ksp632I 219 CTCTTC CTCTTC(1/4)
MamI 594 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 519 GATC ^GATC
MseI 581 TTAA T^TAA
MwoI 427 GCCCTGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 350 CCATGG C^CATGG
NdeII 519 GATC ^GATC
NspI 251 ACATGC [GA]CATG^[CT]
PaeR7I 575 CTCGAG C^TCGAG
PflFI 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 404 TCATGA T^CATGA
Sau3AI 519 GATC ^GATC
SfaNI 23 GCATC GCATC(5/9)
SfcI 867 CTACAG C^T[GA][CT]AG
SspBI 830 TGTACA T^GTACA
StuI 707 AGGCCT AGG^CCT
Tru9I 581 TTAA T^TAA
TseI 66 GCAGC G^C[AT]GC
Tsp509I 660 AATT ^AATT
Tth111I 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 575 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 303 GTAC GT^AC
831 GTAC
Asp700I 855 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
924 GAAAACTTTC
BsaI 323 GAGACC GGTCTC(1/5)
769 GGTCTC
BseRI 127 CTCCTC GAGGAG(10/8)
666 CTCCTC
BsgI 388 CTGCAC GTGCAG(16/14)
506 GTGCAG
BsmFI 6 GGGAC GGGAC(10/14)
682 GTCCC
Cac8I 47 GCCTGC GC[ACGT]^[ACGT]GC
558 GCTGGC
EcoT14I 350 CCATGG C^C[AT][AT]GG
642 CCTTGG
HinfI 154 GACTC G^A[ACGT]TC
908 GAGTC
HphI 591 GGTGA GGTGA(8/7)
840 GGTGA
PleI 154 GACTC GAGTC(4/5)
908 GAGTC
RsaI 303 GTAC GT^AC
831 GTAC
SmlI 575 CTCGAG C^T[CT][GA]AG
676 CTCAAG
StyI 350 CCATGG C^C[AT][AT]GG
642 CCTTGG
TaqI 147 TCGA T^CGA
576 TCGA
TthHB8I 147 TCGA T^CGA
576 TCGA
XmnI 855 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
924 GAAAACTTTC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AvaII BalI BanII
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FokI HaeIII MaeIII MboII MluNI
MnlI MscI MslI MvaI NlaIII
NlaIV PpuMI Sau96I ScrFI Tsp45I
TspRI