Restriction map for
pFN21AE5459
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AvaII AviII AvrII BamHI BbsI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HincII HindII HpaI KspI MaeI
MamI MfeI MflI MluI MroI
MunI MvnI NciI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 631 GGTACC G^GTACC
AcyI 66 GGCGCC G[GA]^CG[CT]C
AflIII 727 ACATGT A^C[GA][CT]GT
AlwNI 8 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 714 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 631 GGTACC G^GTACC
AspHI 94 GTGCTC G[AT]GC[AT]^C
BanII 163 GAGCCC G[GA]GC[CT]^C
BbeI 66 GGCGCC GGCGC^C
BbrPI 667 CACGTG CAC^GTG
BbvCI 135 CCTCAGC CCTCAGC(-5/-2)
BclI 527 TGATCA T^GATCA
BsaAI 667 CACGTG [CT]AC^GT[GA]
BsaHI 66 GGCGCC G[GA]^CG[CT]C
BsiHKAI 94 GTGCTC G[AT]GC[AT]^C
BsmAI 260 GTCTC GTCTC(1/5)
BsmBI 259 CGTCTC CGTCTC(1/5)
BsmFI 267 GTCCC GGGAC(10/14)
BspLU11I 727 ACATGT A^CATGT
BspMI 628 GCAGGT ACCTGC(4/8)
BsrDI 33 CATTGC GCAATG(2/0)
BsrFI 389 GCCGGC [GA]^CCGG[CT]
CfoI 67 GCGC GCG^C
Cfr10I 389 GCCGGC [GA]^CCGG[CT]
DpnII 528 GATC ^GATC
DraII 292 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 484 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 292 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 689 GAATTC G^AATTC
FbaI 527 TGATCA T^GATCA
HaeII 66 GGCGCC [GA]GCGC^[CT]
HapII 390 CCGG C^CGG
HhaI 67 GCGC GCG^C
Hin1I 66 GGCGCC G[GA]^CG[CT]C
HinP1I 67 GCGC G^CGC
HindIII 805 AAGCTT A^AGCTT
HpaII 390 CCGG C^CGG
KasI 66 GGCGCC G^GCGCC
KpnI 631 GGTACC GGTAC^C
MaeII 668 ACGT A^CGT
MboI 528 GATC ^GATC
MseI 779 TTAA T^TAA
MspI 390 CCGG C^CGG
NaeI 389 GCCGGC GCC^GGC
NarI 66 GGCGCC GG^CGCC
NdeII 528 GATC ^GATC
NgoMIV 389 GCCGGC G^CCGGC
NspI 727 ACATGT [GA]CATG^[CT]
PmaCI 667 CACGTG CAC^GTG
PmlI 667 CACGTG CAC^GTG
Sau3AI 528 GATC ^GATC
SexAI 113 ACCTGGT A^CC[AT]GGT
SfoI 66 GGCGCC GGC^GCC
Tru9I 779 TTAA T^TAA
XcmI 321 CCATCTGTTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 714 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 689 GAATTC [GA]^AATT[CT]
833 GAATTT
AfaI 186 GTAC GT^AC
632 GTAC
ApoI 689 GAATTC [GA]^AATT[CT]
833 GAATTT
BalI 380 TGGCCA TGG^CCA
491 TGGCCA
BanI 66 GGCGCC G^G[CT][GA]CC
631 GGTACC
BmyI 94 GTGCTC G[AGT]GC[ACT]^C
163 GAGCCC
BsgI 278 GTGCAG GTGCAG(16/14)
898 GTGCAG
Bsp1286I 94 GTGCTC G[AGT]GC[ACT]^C
163 GAGCCC
DdeI 38 CTGAG C^T[ACGT]AG
136 CTCAG
DsaI 359 CCATGG C^C[GA][CT]GG
638 CCATGG
EaeI 380 TGGCCA [CT]^GGCC[GA]
491 TGGCCA
EcoT14I 359 CCATGG C^C[AT][AT]GG
638 CCATGG
FauI 269 CCCGC CCCGC(4/6)
410 CCCGC
HgaI 233 GCGTC GACGC(5/10)
448 GCGTC
HphI 398 TCACC GGTGA(8/7)
467 TCACC
MluNI 380 TGGCCA TGG^CCA
491 TGGCCA
MscI 380 TGGCCA TGG^CCA
491 TGGCCA
MslI 177 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
408 CACCCGCATG
MspA1I 149 CAGCTG C[AC]G^C[GT]G
674 CAGCTG
NcoI 359 CCATGG C^CATGG
638 CCATGG
PflMI 7 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
483 CCACACTGTGG
PleI 255 GAGTC GAGTC(4/5)
417 GAGTC
PstI 76 CTGCAG CTGCA^G
677 CTGCAG
PvuII 149 CAGCTG CAG^CTG
674 CAGCTG
RsaI 186 GTAC GT^AC
632 GTAC
StyI 359 CCATGG C^C[AT][AT]GG
638 CCATGG
TfiI 10 GAATC G^A[AT]TC
374 GATTC
Tsp509I 690 AATT ^AATT
834 AATT
Van91I 7 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
483 CCACACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I EarI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV SapI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI